Oxydation of oleic acid to (E)-10-hydroperoxy-8-octadecenoic and (E)-10-hydroxy-8-octadecenoic acids by Pseudomonas sp. 42A2

Biochemical Characterization of the Oxygenation of Unsaturated Fatty Acids by the Dioxygenase and Hydroperoxide Isomerase of Pseudomonas aeruginosa 42A2

“…We now know that production of (7S,10S)-DiHOME is a characteristic of P. aeruginosa (13). Strains 42A2 and PR3 also formed 10-hydro(per)oxy-(8E)-octadecenoic acid (10-H(P)OME) (18), and the chirality was determined to be 10S (11). Palmitoleic and ricinoleic acids were also found to be oxygenated in the same way as oleic acid (9,(12)(13)(14).…”

“…We found that the deuterium label of [(8R)- [7,[10][11][12][13][14][15][16] O 2 ]7,10-DiHOME shows a carboxylate anion at m/z 313, whereas [7,[10][11][12][13][14][15][16][17][18] O 2 ]7,10-DiHOME has the corresponding ion at m/z 317. We could not detect [7,10-16 O 18 O]7,10-DiHOME at m/z 315, suggesting that both hydroxyl oxygens were derived from the same molecule of O 2 .…”

A second type III secretion system in Burkholderia pseudomallei: who is the real culprit?

“…Burkholderia pseudomallei is a Gram-negative motile bacillus that is the causative agent of melioidosis, a severe emerging infection that is endemic in South-East Asia and Northern Australia. Antibiotic therapy of melioidosis is long and difficult, because of the resistance of the bacterium to many antibiotics and a tendency to relapse after recovery from clinical disease (3,4). Although some potential virulence factors have been suggested, the pathogenesis of the disease is poorly understood (1).…”

Genetic transfer and genome evolution in MRSA

“…essential, genes) represents a portable, precise and sensitive method for typing MRSA strains (3). The sequence data for parts of the seven housekeeping genes from the whole genome sequences of the five strains listed in Table 1b is included as a standard because of the easy availability of MLST data for a large number of MRSA isolates (3). When the five genomes are compared, the total number of SNPs (31\2645) in all ISR types (Table 1a) is similar to the total number of SNPs (43\3818) found in the seven housekeeping genes (Table 1b).…”

“…The data presented here suggest that at least three mechanisms of genome evolution in MRSA exist including (i) recombination and\or gene conversion of the rrn operon resulting in sequence homogeneity in some regions and rearrangements, insertions or deletions in others (5, 17), (ii) recombination of essential housekeeping genes (4) and (iii) horizontal transfer (2,13,15,16 Burkholderia pseudomallei is a Gram-negative motile bacillus that is the causative agent of melioidosis, a severe emerging infection that is endemic in South-East Asia and Northern Australia. Antibiotic therapy of melioidosis is long and difficult, because of the resistance of the bacterium to many antibiotics and a tendency to relapse after recovery from clinical disease (3,4). Although some potential virulence factors have been suggested, the pathogenesis of the disease is poorly understood (1).…”