2024
DOI: 10.1002/ange.202317711
|View full text |Cite
|
Sign up to set email alerts
|

Oxygen‐18 Labeling Reveals a Mixed Fe−O Mechanism in the Last Step of Cytochrome P450 51 Sterol 14α‐Demethylation

Kevin D. McCarty,
Yasuhiro Tateishi,
Tatiana Y. Hargrove
et al.

Abstract: The 14α‐demethylation step is critical in eukaryotic sterol biosynthesis, catalyzed by cytochrome P450 (P450) Family 51 enzymes, e.g. with lanosterol in mammals. This conserved 3‐step reaction terminates in a C‐C cleavage step that generates formic acid, the nature of which has been controversial. Proposed mechanisms involve roles of P450 Compound 0 (ferric peroxide anion, FeO2¯) or Compound I (perferryl oxygen, FeO3+) reacting with either the aldehyde or its hydrate, respectively. Analysis of 18O incorporatio… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

0
0
0

Publication Types

Select...

Relationship

0
0

Authors

Journals

citations
Cited by 0 publications
references
References 66 publications
0
0
0
Order By: Relevance

No citations

Set email alert for when this publication receives citations?