Oxygen consumption by Day 7 bovine blastocysts: determination of ATP production

Thompson, Jeremy G.; Partridge, R.J.; Houghton, Franchesca D.; Kennedy, Christopher J.; Pullar, D.; Leese, Henry J.

doi:10.1016/0378-4320(96)01477-7

Cited by 10 publications

(5 citation statements)

References 24 publications

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“…9B). With excess oxygen, 40% of the ATP demand of ES cells was satisfied through anaerobic glycolysis, which is higher than the 15-25% reported for early embryos (Houghton, 2006;Houghton et al, 1996;Thompson et al, 1996). The reliance on anaerobic metabolism increased further with oxygen depletion, and all of the ATP was produced anaerobically under anoxia.…”

Section: Discussionmentioning

confidence: 75%

“…Mouse, bovine, and human blastocysts have high glycolytic capacity and can readily convert glucose into lactic acid (Devreker and Englert, 2000;Harvey et al, 2002;Hewitson and Leese, 1993;Houghton et al, 1996). Nonetheless, 75-85% of the ATP production in embryos occurs through oxidative phosphorylation at high pO 2gas (Houghton, 2006;Houghton et al, 1996;Thompson et al, 1996). The high levels of glycolytic enzymes in ES cells during culture at a pO 2gas of 142 mmHg are further increased at reduced pO 2gas conditions through the action of HIF-1a (Iyer et al, 1998;Wenger, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Effects of oxygen on mouse embryonic stem cell growth, phenotype retention, and cellular energetics

Powers

Millman

Huang

et al. 2008

Biotech & Bioengineering

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Most embryonic stem (ES) cell research is performed with a gas phase oxygen partial pressure (pO 2 ) of 142 mmHg, whereas embryonic cells in early development are exposed to pO 2 values of 0-30 mmHg. To understand effects of these differences, we studied murine ES (mES) growth, maintenance of stem cell phenotype, and cell energetics over a pO 2 range of 0-285 mmHg, in the presence or absence of differentiation-suppressing leukemia inhibitory factor (LIF). With LIF, growth rate was sensitive to pO 2 but constant with time, and expression of self-renewal transcription factors decreased at extremes of pO 2 . Subtle morphological changes suggested some early differentiation, but cells retained the ability to differentiate into derivatives of all three germ layers at low pO 2 . Without LIF, growth rate decreased with time, and self-renewal transcription factor mRNA decreased further. Gross morphological changes occurred, and overt differentiation occurred at all pO 2 . These findings suggested that hypoxia in the presence of LIF promoted limited early differentiation. ES cells survived oxygen starvation with negligible cell death by increasing anaerobic metabolism within 48 h of anoxic exposure. Decreasing pO 2 to 36 mmHg or lower decreased oxygen consumption rate and increased lactate production rate. The fraction of ATP generated aerobically was 60% at or above 142 mmHg and decreased to 0% under anoxia, but the total ATP production rate remained nearly constant at all pO 2 . In conclusion, undifferentiated ES cells adapt their energy metabolism to proliferate at all pO 2 between 0 and 285 mmHg. Oxygen has minimal effects on undifferentiated cell growth and phenotype, but may exert more substantial effects under differentiating conditions.

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Effects of oxygen on mouse embryonic stem cell growth, phenotype retention, and cellular energetics

Powers

Millman

Huang

et al. 2008

Biotech & Bioengineering

View full text Add to dashboard Cite

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“…Since at the later stage of blastocyst the decrease of ROS was detected, this may be related to onset of cellular differentiation. It has been demonstrated that, oxygen uptake diminishes in expanded blastocysts due to their lower physiological ATP demand [46], because glycolysis begins to contribute to ATP production. Moreover, shifts in intracellular REDOX state may also contribute to spatial differences in cell activity, especially after compaction, and perhaps even major embryonic events such as fertilization, genome activation and cellular differentiation [47].…”

Section: Discussionmentioning

confidence: 99%

Ameliorating effect of vitamin E on in vitro development of preimplantation buffalo embryos

Thiyagarajan

Valivittan

2009

J Assist Reprod Genet

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“…ROS levels gradually increase from the two‐cell embryo to the morula stage (Dalvit, Cetica, Pintos, & Beconi, ), possibly as a consequence of metabolic changes toward oxidative processes that occur during embryonic development. Indeed, Thompson, Partridge, Houghton, Cox, and Leese () reported a sustained increase in oxygen, glucose and pyruvate uptake during early embryonic development in vitro, although oxygen uptake diminishes in expanded blastocysts due to their lower physiological ATP demand (Thompson, Partridge, Houghton, Kennedy, Pullar, & Leese, ) Similarly, Dalvit, Cetica, Pintos, and Beconi () reported that in vitro‐produced 2‐ and 4‐cell embryos metabolize amino acids and other biomolecules at a high rate, although this rate declines at the later preimplantation embryo stage. The lower metabolic rates of blastocysts may be a consequence of the cellular differentiation that occurs during this transitional period of development.…”

Section: Discussionmentioning

confidence: 99%

Improved developmental competence in embryos treated with lycopene during in vitro culture system

Chowdhury

Mesalam

Khan

et al. 2018

Molecular Reproduction Devel

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In vitro embryo development remains suboptimal compared to in vivo development due to the challenge from various stressors associated with in vitro culturing of oocytes. When 0.2 μM lycopene was added to oocyte in vitro maturation and embryo culture media, to assess its antioxidant effects on embryo development, we observed a significant (p < 0.05) increase in cleavage and blastocyst development rates compared to the corresponding controls (84.3 ± 0.6% vs. 73.1 ± 1.9% and 41.0 ± 1.4% vs. 33.4 ± 0.7%, respectively). Lycopene also significantly reduced (p < 0.05) intracellular reactive oxygen species concentrations in oocytes and blastocysts, whereas lipid peroxidation and mitochondrial activity increased compared to control conditions. The number of apoptotic nuclei was significantly reduced in the lycopene-treated compared to the control group (1.7 ± 0.1 vs. 4.7 ± 0.3), and the quantity of cells in the trophectoderm (207.1 ± 1.6 vs. 171.3 ± 1.0, respectively) and inner cell mass (41.9 ± 0.4 vs. 36.7 ± 0.4, respectively) was higher following treatment-although the inner cell mass-to-trophectoderm ratio was unchanged (1:3.3 vs. 1:3.4 for lycopene vs. control, respectively). Lycopene supplementation also significantly (p < 0.05) attenuated expression of IKBKB (Inhibitor of nuclear factor kappa B kinase, subunit beta) and reduced Caspase 9 and Caspase 3 protein abundance, while up-regulating GDF9 (Growth and differentiation factor 9), BMP15 (Bone morphogenetic protein 15), SOD2 (Superoxide dismutase 2), NDUFA2 (NADH dehydrogenase), ACADL (Acyl-CoA dehydrogenase, long chain), and ACSL3 (Acyl-CoA synthetase 3, long-chain membrane 3) transcription compared to control. Therefore, co-culturing with lycopene during oocyte maturation improved bovine embryo developmental potential during in vitro culture by improving embryonic resilience to stress.

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Oxygen consumption by Day 7 bovine blastocysts: determination of ATP production

Cited by 10 publications

References 24 publications

Effects of oxygen on mouse embryonic stem cell growth, phenotype retention, and cellular energetics

Effects of oxygen on mouse embryonic stem cell growth, phenotype retention, and cellular energetics

Ameliorating effect of vitamin E on in vitro development of preimplantation buffalo embryos

Improved developmental competence in embryos treated with lycopene during in vitro culture system

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