Oxygen Diffusion Constants D and K of Tumor Tissue (DS-Carcinosarcoma) and Their Temperature Dependence

Grote, J.; Süsskind, R.; Vaupel, Peter

doi:10.1007/978-1-4684-8890-6_47

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…The diffusion distances established in a multicell tumour spheroid are characteristic of intercapillary distances in tumours. Furthermore, the diffusion coefficient that we have measured for EMT6Ro spheroids compares favourably to that measured by Grote et al (1977) for tumour tissue. From experiment we have established a quantitative relationship between the rate of photochemicd '04 consumption and the radiation fluence rate, For Photofrinm and 630 nm irradiatlon, the value of BpoT in thespheroid system is approximately 0.79 pmol I-' s-' mW-' cm2.…”

Section: Discussionsupporting

confidence: 73%

“…For our purposes, it is sufficient that the diffusion parameters are truly representative of the spheroid in an environment that closely resembles that of experiments described previously (Foster er al 1993). The differences between this study and Mueller-Klieser's notwithstanding, it is interesting to note that there is a better agreement between the diffusion coefficient reported here and that reported by Grote for DS-carcinosarcoma tissue (Grote et al 1977).…”

Section: Microelectrode Measurementscontrasting

confidence: 50%

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Oxygen diffusion and reaction kinetics in the photodynamic therapy of multicell tumour spheroids

1994

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Effects of oxygen diffusion and reaction kinetics in photodynamic therapy are considered in the context of a multicell tumour spheroid model. Steady-state measurements of oxygen made with a Clark-style microelectrode (4 microm diameter tip) enable us to determine the rate of metabolic oxygen consumption and the oxygen diffusion coefficient in 500 microm diameter EMT6/Ro spheroids. These values are 5.77 micromol 1(-1) s(-1) and 1460 microm2 s(-1), respectively. Time-dependent electrode measurements of oxygen concentration during laser irradiation of individual Photofrin-sensitized spheroids are fitted to numerical solutions of a pair of diffusion-with-reaction equations. The analysis yields the rate of photodynamic oxygen consumption and a parameter that governs the oxygen sensitivity of photodynamic therapy. These experimentally derived quantities are used to calculate the temporal and spatial distributions of oxygen and the rate of oxygen consumption in a spheroid during irradiation at several fluence rates. The spatial distribution of photodynamic oxygen consumption is strongly fluence rate dependent. Using the experimental and theoretical results developed in this report, previously published survival data are analysed. The analysis indicates that the threshold dose of reacting singlet oxygen in the EMT6/Ro spheroid is 323 +/- 38 micromol 1(-1) (mean +/- SEM).

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Section: Discussionsupporting

confidence: 73%

Section: Microelectrode Measurementscontrasting

confidence: 50%

Oxygen diffusion and reaction kinetics in the photodynamic therapy of multicell tumour spheroids

1994

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“…The primary driving force for tumor angiogenesis is the combination of the demand for oxygen and nutrients by the growing cancer cells, and the physical limit of the distances for small molecules to diffuse across the stroma between a nearby capillary and the cell making the demand, a physical limit of about 100 mm [3]. Folkman and colleagues suggested that once the radius of the tumor reaches this limit, hypoxic conditions occur in the center of the cell mass.…”

Section: Introductionmentioning

confidence: 99%

The enigmatic role of angiopoietin-1 in tumor angiogenesis

Metheny-Barlow

2003

Cell Res

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A tumor vasculature is highly unstable and immature, characterized by a high proliferation rate of endothelial cells, hyper-permeability, and chaotic blood flow. The dysfunctional vasculature gives rise to continual plasma leakage and hypoxia in the tumor, resulting in constant on-sets of inflammation and angiogenesis. Tumors are thus likened to wounds that will not heal. The lack of functional mural cells, including pericytes and vascular smooth muscle cells, in tumor vascular structure contributes significantly to the abnormality of tumor vessels. Angiopoietin-1 (Ang1) is a physiological angiogenesis promoter during embryonic development. The function of Ang1 is essential to endothelial cell survival, vascular branching, and pericyte recruitment. However, an increasing amount of experimental data suggest that Ang1-stimulated association of mural cells with endothelial cells lead to stabilization of newly formed blood vessels. This in turn may limit the otherwise continuous angiogenesis in the tumor, and consequently give rise to inhibition of tumor growth. We discuss the enigmatic role of Ang1 in tumor angiogenesis in this review.

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Master Role of Hypoxia in Cancer Progression: Major Insights During ISOTT’s Half-Century

Vaupel,

Semenza

2024

Advances in Experimental Medicine and Biology

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Oxygen Diffusion Constants D and K of Tumor Tissue (DS-Carcinosarcoma) and Their Temperature Dependence

Cited by 5 publications

References 11 publications

Oxygen diffusion and reaction kinetics in the photodynamic therapy of multicell tumour spheroids

Oxygen diffusion and reaction kinetics in the photodynamic therapy of multicell tumour spheroids

The enigmatic role of angiopoietin-1 in tumor angiogenesis

Master Role of Hypoxia in Cancer Progression: Major Insights During ISOTT’s Half-Century

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