Oxygen distribution in murine tumors: characterization using oxygen-dependent quenching of phosphorescence

Ziemer, Lisa S.; Lee, William M. F.; Vinogradov, Sergei A.; Sehgal, Chandra M.; Wilson, David F.

doi:10.1152/japplphysiol.01140.2004

Cited by 79 publications

(75 citation statements)

References 42 publications

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“…We studied acid aspiration (15,54), because it occurs commonly and is associated with a relatively high mortality (52,54,59). The model is reproducible, reliably generates an inflammatory response involving PMNs, increases microvascular permeability, and causes alveolar edema (15,54).…”

Section: Discussionmentioning

confidence: 99%

“…Continuous monitoring of oxygen tension in the systemic blood was measured using a noninvasive phosphorescence quenching method (52,53) (additional methodological details are provided in the online supplement).…”

Section: Systemic Oxygenationmentioning

confidence: 99%

“…Therefore, we followed Pa O 2 in real-time using a noninvasive phosphorescence quenching technique that obviates the need for repetitive arterial blood sampling (52,53). WT mice were given intratracheal Fc-RAP or BSA and then injected intravenously with a 100-ml solution containing 200 mM oxyphor G3.…”

Section: Fc-rap Attenuates Hypoxemia After Alimentioning

confidence: 99%

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Neutrophil α-Defensins Cause Lung Injury by Disrupting the Capillary–Epithelial Barrier

Bdeir¹,

Higazi²,

Kulikovskaya³

et al. 2010

Am J Respir Crit Care Med

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Rationale: The involvement of neutrophil activation in the sentinel, potentially reversible, events in the pathogenesis of acute lung injury (ALI) is only partially understood. a-Defensins are the most abundant proteins secreted by activated human neutrophils, but their contribution to ALI in mouse models is hindered by their absence from murine neutrophils and the inability to study their effects in isolation in other species. Objectives: To study the role of a-defensins in the pathogenesis of ALI in a clinically relevant setting using mice transgenic for polymorphonuclear leukocyte expression of a-defensins. Methods: Transgenic mice expressing polymorphonuclear leukocyte a-defensins were generated. ALI was induced by acid aspiration. Pulmonary vascular permeability was studied in vivo using labeled dextran and fibrin deposition. The role of the low-density lipoproteinrelated receptor (LRP) in permeability was examined. Measurements and Main Results: Acid aspiration induced neutrophil migration and release of a-defensins into lung parenchyma and airways. ALI was more severe in a-defensin-expressing mice than in wild-type mice, as determined by inspection, influx of neutrophils into the interstitial space and airways, histological evidence of epithelial injury, interstitial edema, extravascular fibrin deposition, impaired oxygenation, and reduced survival. Within 4 hours of insult, a-defensinexpressing mice showed greater disruption of capillary-epithelial barrier function and ALI that was attenuated by systemic or intratracheal administration of specific inhibitors of the LRP. Conclusions: a-Defensins mediate ALI through LRP-mediated loss of capillary-epithelial barrier function, suggesting a potential new approach to intervention.

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Section: Discussionmentioning

confidence: 99%

Section: Systemic Oxygenationmentioning

confidence: 99%

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Neutrophil α-Defensins Cause Lung Injury by Disrupting the Capillary–Epithelial Barrier

Bdeir¹,

Higazi²,

Kulikovskaya³

et al. 2010

Am J Respir Crit Care Med

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“…Quenching of phosphorescence by oxygen affects the phosphorescence lifetime of such sensors, which then enables measurement of oxygen partial pressure in vivo in tissues or thick biological samples with high temporal and spatial resolution [13][14][15]. PLIM-based partial oxygen measurements can be used to quantify the degree of hypoxic tissues or tumors, a critical physiological parameter of solid tumors that determines tumor growth, gene expression [16], metastatic potential [17], metabolism, prognosis [18][19][20], and response to therapy [21,22].…”

Section: Introductionmentioning

confidence: 99%

3D-resolved fluorescence and phosphorescence lifetime imaging using temporal focusing wide-field two-photon excitation

Choi¹,

Tzeranis²,

Won³

et al. 2012

Opt. Express

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Fluorescence and phosphorescence lifetime imaging are powerful techniques for studying intracellular protein interactions and for diagnosing tissue pathophysiology. While lifetime-resolved microscopy has long been in the repertoire of the biophotonics community, current implementations fall short in terms of simultaneously providing 3D resolution, high throughput, and good tissue penetration. This report describes a new highly efficient lifetime-resolved imaging method that combines temporal focusing wide-field multiphoton excitation and simultaneous acquisition of lifetime information in frequency domain using a nanosecond gated imager from a 3D-resolved plane. This approach is scalable allowing fast volumetric imaging limited only by the available laser peak power. The accuracy and performance of the proposed method is demonstrated in several imaging studies important for understanding peripheral nerve regeneration processes. Most importantly, the parallelism of this approach may enhance the imaging speed of long lifetime processes such as phosphorescence by several orders of magnitude. 291-295 (1996). 6. K. König, P. T. So, W. W. Mantulin, B. J. Tromberg, and E. Gratton, "Two-photon excited lifetime imaging of autofluorescence in cells during UVA and NIR photostress," J. Microsc. 183(Pt 3), 197-204 (1996). 7. J. R. Lakowicz, "Emerging applications of fluorescence spectroscopy to cellular imaging: lifetime imaging, metal-ligand probes, multi-photon excitation and light quenching," Scanning Microsc. Suppl. 10, 213-224 (1996 high-resolution measurements reveal a lack of correlation," Nat. Med. 3(2), 177-182 (1997). 14. I. P. Torres Filho, M. Leunig, F. Yuan, M. Intaglietta, and R. K. Jain, "Noninvasive measurement of microvascular and interstitial oxygen profiles in a human tumor in SCID mice," Proc. Natl. Acad. Sci. U.S.A. French, "Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source," Opt. Lett. 32(23), 3408-3410 (2007). 32.

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“…The group of Wilson and coworkers (8,47) have used extracellular probes based on phosphorescent dyes palladium(II) tetracarboxyphenyl porphyrin and palladium(II) tetrabenzo porphyrin (PdTBP) in low-resolution lifetime-based imaging of tissue and tumor oxygenation. A similar principle has been applied to develop simple formats for measuring biological oxygen consumption in microtiter plates on a fluorescent plate reader using phosphorescent solid-state oxygen sensors (18,27) and water-soluble probes (11).…”

mentioning

confidence: 99%

Sensing intracellular oxygen using near-infrared phosphorescent probes and live-cell fluorescence imaging

O’Riordan¹,

Fitzgerald²,

Ponomarev³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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Papkovsky DB. Sensing intracellular oxygen using near-infrared phosphorescent probes and live-cell fluorescence imaging. Am J Physiol Regul Integr Comp Physiol 292: R1613-R1620, 2007. First published December 14, 2006; doi:10.1152/ajpregu.00707.2006.-The development and application of a methodology for measurement of oxygen within single mammalian cells are presented, which employ novel macromolecular near infrared (NIR) oxygen probes based on new metalloporphyrin dyes. The probes, which display optimal spectral characteristics and sensitivity to oxygen, excellent photostability, and low cytotoxicity and phototoxicity, are loaded into cells by simple transfection procedures and subsequently analyzed by high-resolution fluorescence microscopy. The methodology is demonstrated by sensing intracellular oxygen in different mammalian cell lines, including A549, Jurkat, and HeLa, and monitoring rapid and transient changes in response to mitochondrial uncoupling by valinomycin and inhibition by antimycin A. Furthermore, the effect of ryanodine receptormediated Ca 2ϩ influx on cellular oxygen uptake is shown by substantial changes in the level of intracellular oxygen. The results demonstrate the ability of this technique to measure small, rapid, and transient changes in intracellular oxygen in response to different biological effectors. Moreover, this technique has wide ranging applicability in cell biology and is particularly useful in the study of low oxygen environments (cellular hypoxia), mitochondrial and cellular (dys)function, and for therapeutic areas, such as cardiovascular and neurological research, metabolic diseases, and cancer. metalloporphyrin; mitochondrial function; uncoupling MOLECULAR OXYGEN IS A KEY substrate in aerobic biological systems, providing the terminal electron acceptor of the electron transport chain (7). The rate of oxygen consumption is closely regulated by the ATP/ADP ratio (24), while also being highly dependent on the concentration of available oxygen (31) and the action of signaling molecules such as NO (9, 37) and Ca 2ϩ (10, 23), which, in turn, are related to pathways of cell survival and death (22). Furthermore, the induction of cellular hypoxia results in the activation of a specific adaptive transcriptional response governed by the hypoxia inducible transcription factors (9). Oxygen consumption is, therefore, an informative marker of cellular metabolism, which is broadly applicable to various biological systems from mitochondria to cells to whole organisms. It may be used to elucidate biochemical pathways of the cell and alterations in metabolism caused by various stimuli or disease states. Accordingly, there is a requirement for methods that can effectively monitor cellular oxygen levels and oxygen consumption rates.Optical schemes, based on the quenching by molecular oxygen of long-decay fluorescent and phosphorescent dyes, such as metalloporphyrins and ruthenium(II) complexes, have been under active development in recent years (32). The group of Wilson and coworkers (8, 47) ha...

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Oxygen distribution in murine tumors: characterization using oxygen-dependent quenching of phosphorescence

Cited by 79 publications

References 42 publications

Neutrophil α-Defensins Cause Lung Injury by Disrupting the Capillary–Epithelial Barrier

Neutrophil α-Defensins Cause Lung Injury by Disrupting the Capillary–Epithelial Barrier

3D-resolved fluorescence and phosphorescence lifetime imaging using temporal focusing wide-field two-photon excitation

Sensing intracellular oxygen using near-infrared phosphorescent probes and live-cell fluorescence imaging

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