Oxygen, early embryonic metabolism and free radical-mediated embryopathies

Burton, Graham J.; Hempstock, Joanne; Jauniaux, Eric

doi:10.1016/s1472-6483(10)62060-3

Cited by 226 publications

(142 citation statements)

References 100 publications

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“…31,32 In addition, it is widely recognized that placental and embryonic tissues are particularly sensitive to oxidative stress, as a consequence of the high cell proliferation rate and concomitant exposure of DNA. 33 For such reasons it has been proposed that the physiological hypoxia characterizing the first trimester of human gestation might be a way to protect the fetus from teratogenic effects of reactive oxygen species (ROS). 34 The presence of extensive vascular alterations and of thrombi in our model system ( Figure 3DÀG) led us to hypothesize that placental damage might be a consequence of oxidative stress.…”

Section: Articlementioning

confidence: 99%

Low Doses of Pristine and Oxidized Single-Wall Carbon Nanotubes Affect Mammalian Embryonic Development

Massimiani²,

et al. 2011

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Several in vitro and in vivo studies suggest local and systemic effects following\ud exposure to carbon nanotubes. No data are available, however, on their possible\ud embryotoxicity in mammals. In this study, we tested the effect of pristine and\ud oxidized single-wall carbon nanotubes (SWCNTs) on the development of the mouse\ud embryo. To this end, SWCNTs (from 10 ng to 30 μg/mouse) were administered to\ud female mice soon after implantation (postcoital day 5.5); 10 days later, animals \ud were sacrificed, and uteri, placentas, and fetuses examined. A high percentage of\ud early miscarriages and fetal malformations was observed in females exposed to\ud oxidized SWCNTs, while lower percentages were found in animals exposed to the\ud pristine material. The lowest effective dose was 100 ng/mouse. Extensive vascular\ud lesions and increased production of reactive oxygen species (ROS) were detected\ud in placentas of malformed but not of normally developed fetuses. Increased ROS\ud levels were likewise detected in malformed fetuses. No increased ROS production\ud or evident morphological alterations were observed in maternal tissues. No fetal \ud and placental abnormalities were ever observed in control animals. In parallel,\ud SWCNT embryotoxicity was evaluated using the embryonic stem cell test (EST), a\ud validated in vitro assay developed for predicting embryotoxicity of soluble\ud chemical compounds, but never applied in full to nanoparticles. The EST predicted\ud the in vivo data, identifying oxidized SWCNTs as the more toxic compound

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Section: Articlementioning

confidence: 99%

Low Doses of Pristine and Oxidized Single-Wall Carbon Nanotubes Affect Mammalian Embryonic Development

Massimiani²,

et al. 2011

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“…Our findings, using a first trimester cytotrophoblast cell line, reveal that the protective activity of endogenous HBEGF is lost when O 2 levels are abruptly increased. The resulting cell death requires caspase activity and is accompanied by phosphatidylserine randomization, while cells remain intact and retain cytoplasmic LDH activity; all indicative of apoptosis (14) It has been suggested that excessive oxidative stress in the first trimester can compromise trophoblast function or survival (1)(2)(3)(4)(5)9). Trophoblast survival and extravillous differentiation required for remodeling of the uterine spiral arteries are deficient in both missed abortion and preeclampsia (1), perhaps due to elevated levels of oxidative stress.…”

Section: Commentmentioning

confidence: 99%

“…A role for H/R injury in early pregnancy loss has been advanced by several lines of molecular evidence, including morphological evidence of increased apoptosis and markers of cellular stress, expression of heat shock protein 70, protein nitrosylation and lipid peroxidation in tissues obtained from missed abortions with early onset placental perfusion (1)(2)(3)(4)(5). Xanthine dehydrogenase, which normally utilizes NAD as an electron acceptor, is converted under the conditions of ischemia/reperfusion into xanthine oxidase.…”

Section: Commentmentioning

confidence: 99%

“…The possible role of H/R in early pregnancy loss has been advanced by several lines of evidence. These include premature onset of the maternal circulation to the placenta, determined by Doppler ultrasonography and correlated to fragmented trophoblastic shell, limited EVT invasion of the placental bed and terminal spiral arteries (1)(2)(3)(4)(5). The effect is greatest in pregnancies less than 10 weeks gestation with recent fetal demise suggesting that early gestations are more susceptible to this damage.…”

Section: Introductionmentioning

confidence: 99%

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Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor

Leach

Kilburn

Petkova

et al. 2008

American Journal of Obstetrics and Gynecology

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Objective-The anti-apoptotic action of HBEGF and its regulation by O 2 constitutes a key factor for trophoblast survival. The hypothesis that cytotrophoblast survival is compromised by exposure to hypoxia-reoxygenation (H/R) injury, which may contribute to preeclampsia and some missed abortions, prompted us to investigate HBEGF regulation and its role as a survival factor during H/ R in cytotrophoblast cells Study Design-A transformed human first trimester cytotrophoblast cell line HTR-8/SVneo was exposed to H/R (2% O 2 followed by 20% O 2 ) and assessed for HBEGF expression and cell death.Results-Cellular HBEGF declined significantly within 30 minutes of reoxygenation after culture at 2% O 2 . H/R significantly reduced proliferation and increased cell death when compared to trophoblast cells cultured continuously at 2% or 20% O 2 . Restoration of cell survival also was achieved by adding recombinant HBEGF during reoxygenation. HBEGF inhibited apoptosis through its binding to either HER1 or HER4, its cognate receptors.Conclusion-These results provide evidence that cytotrophoblast exposure to H/R induces apoptosis and decreased cell proliferation. HBEGF accumulation is diminished under these conditions, while restoration of HBEGF signaling improves trophoblast survival.

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“…As mentioned above, PO2 and ROS might be essential parameters at least in early embryogenesis [42]. The discrepancies in the results of the above-mentioned studies may be partially explained by differences in culture hardware as well as culture methods: for example, oxygen tension in droplets of medium under oil will be 1ess than those without an oil overlay [43].…”

Section: Delivery Of Human Babies By Assisted Reproductive Technologymentioning

confidence: 96%

Cell Handling and Culture Under Controlled Oxygen Concentration

Kaneko¹,

Takamatsu²

2012

Biomedical Tissue Culture

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Oxygen, early embryonic metabolism and free radical-mediated embryopathies

Cited by 226 publications

References 100 publications

Low Doses of Pristine and Oxidized Single-Wall Carbon Nanotubes Affect Mammalian Embryonic Development

Low Doses of Pristine and Oxidized Single-Wall Carbon Nanotubes Affect Mammalian Embryonic Development

Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor

Cell Handling and Culture Under Controlled Oxygen Concentration

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