Oxygen effect on batch cultures of Leuconostoc mesenteroides: relationship between oxygen uptake, growth and end-products

Plihon, Florence; Taillandier, Patricia; Strehaiano, Pierre

doi:10.1007/bf00170632

Cited by 19 publications

(9 citation statements)

References 16 publications

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“…However, Lucey & Condon (1986) and Sakamoto & Komagata (1996) found that Leuconostoc mesenteroides cultured aerobically grew better than when cultured anaerobically because of the presence of inducible NAD(P)H oxidases, leading the cells to gain an ATP molecule from the transformation of acetyl phosphate to acetate. Plihon et al (1995) also reported an improvement in Leuconostoc mesenteroides growth under aerobic conditions when the culture was supplied with sparged air or pure O # . In some cases, LAB do not benefit from O # , but they are not inhibited by its presence, e.g.…”

Section: Effect Of Anaerobic or Aerobic Conditions On Growth And Metamentioning

confidence: 90%

“…NAD(P)H produced during the first steps of the heterolactic catabolism of sugars is reoxidized by the conversion of pyruvic acid to lactic acid, as well as by the conversion of acetyl phosphate to ethanol. Although lactic acid bacteria (LAB) have a fermentative metabolism, some Leuconostoc strains exhibit higher biomass production when cultured in the presence of O # (Plihon et al, 1995). Under aerobic conditions, O # acts as an electron acceptor oxidizing NADP(H) by NAD(P)H oxidases.…”

Section: Introductionmentioning

confidence: 99%

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NAD(P)H regeneration is the key for heterolactic fermentation of hexoses in Oenococcus oeni

2002

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Oenococcus oeni (formerly Leuconostoc oenos) can perform malolactic fermentation, converting L-malate to L-lactate and carbon dioxide, in wines. The energy and redox potential required to support the growth of the microorganism are supplied mainly by the consumption of carbohydrates via the heterolactic pathway. In the first steps of hexose metabolism two molecules of NAD(P) M are consumed, which must be regenerated in later reactions. The aim of this work was to test if aerobic growth of O. oeni promotes higher cell yields than anaerobic conditions, as has been shown for other lactic acid bacteria. O. oeni M42 was found to grow poorly under aerobic conditions with glucose as the only carbohydrate in the medium. It was demonstrated that O 2 inactivates the enzymes of the ethanol-forming pathway, one of the two pathways which reoxidizes NAD(P) M cofactors in the heterolactic catabolism of glucose. These results suggest that the regeneration of cofactors is the limiting factor for the aerobic consumption of glucose. When external electron acceptors, such as fructose or pyruvate, were added to glucose-containing culture medium the growth of O. oeni was stimulated slightly ; fructose was converted to mannitol, oxidizing two molecules of NAD(P)H, and pyruvate was transformed to lactate, enabling the regeneration of NAD M . The addition of cysteine seemed to suppress the inactivation of the ethanol-forming pathway enzymes by O 2 , enabling glucose consumption in aerobic conditions to reach similar rates to those found in anaerobic conditions.

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Section: Effect Of Anaerobic or Aerobic Conditions On Growth And Metamentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

NAD(P)H regeneration is the key for heterolactic fermentation of hexoses in Oenococcus oeni

2002

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“…In the presence of oxygen or auxiliary substrates (e.g., fructose, citrate, and pyruvate), increased amounts of acetate are produced at the expense of ethanol. Production of acetate rather than ethanol allows the synthesis of additional ATP via acetate kinase and leads to higher specific growth rates (s) (5,13,21,24,25,29). Garvie (8) mentioned that many Leuconostoc species prefer fructose to glucose for growth.…”

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confidence: 99%

Growth and energetics of Leuconostoc mesenteroides NRRL B-1299 during metabolism of various sugars and their consequences for dextransucrase production

Dols

Chraibi

Remaud‐Siméon

et al. 1997

Appl Environ Microbiol

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The metabolic and energetic properties of Leuconostoc mesenteroides have been examined with the goal of better understanding the parameters which affect dextransucrase activity and hence allowing the development of strategies for improved dextransucrase production. Glucose and fructose support equivalent specific growth rates (0.6 h ؊1) under aerobic conditions, but glucose leads to a better biomass yield in anaerobiosis. Both sugars are phosphorylated by specific hexokinases and catabolized through the heterofermentative phosphoketolase pathway. During sucrose-grown cultures, a large fraction of sucrose is converted outside the cell by dextransucrase into dextran and fructose and does not support growth. The other fraction enters the cell, where it is phosphorylated by an inducible sucrose phosphorylase and converted to glucose-6-phosphate (G-6-P) by a constitutive phosphoglucomutase and to heterofermentative products (lactate, acetate, and ethanol). Sucrose supports a higher growth rate (0.98 h ؊1) than the monosaccharides. When fructose is not consumed simultaneously with G-1-P, the biomass yield relative to ATP is high (16.8 mol of ATP ⅐ mol of sucrose ؊1), and dextransucrase production is directly proportional to growth. However, when the fructose moiety is used, a sink of energy is observed, and dextransucrase production is no longer correlated with growth. As a consequence, fructose catabolism must be avoided to improve the amount of dextransucrase synthesized.

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“…Furthermore carbon balances on fructose indicate that additional unidentified fermentation products, not seen on other sugars are being produced. Conversely, the highest biomass production was obtained on sucrose which coincided with the highest acetic acid production suggesting that energy supply as ATP could be determining growth efficiency as demonstrated in heterofermentative lactic acid bacteria (Plihon et al. 1995).…”

Section: Discussionmentioning

confidence: 99%

Fructose and glucose mediates enterotoxin production and anaerobic metabolism ofBacillus cereusATCC14579^T

Ouhib-Jacobs

Lindley

Schmitt

et al. 2009

Journal of Applied Microbiology

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Aims: To determine the effects of carbohydrates on Bacillus cereus ATCC14579T anaerobic metabolism and enterotoxin production in amino acids rich medium. Methods and Results: Bacillus cereus anaerobic growth on different carbohydrates (glucose, fructose, sucrose or glucose–fructose mixture) was examined in synthetic mMOD medium under continuous cultures (μ = 0·2 h−1). Fermentation end‐products, flux partitioning at each key branch points of the mixed acid pathway and consumption or production of amino acids were determined. On both fructose and sucrose, ATP production was favoured via acetate production from acetyl‐CoA. In addition, amino acids present in the growth medium showed significant variations with high consumption of serine and net production of glutamate and alanine on some or all sugars. Enterotoxins Hbl and Nhe production was high during growth on fructose (or mixtures involving a fructose moiety). Conclusions: Fructose was identified as a key sugar influencing anaerobic metabolism and toxin production of B. cereus. Significance and Impact of the Study: The physiological differences associated with the fermentation of the various carbohydrates clearly modify toxinogenesis indicating that the risk of foodborne pathogens is to some extent dependent upon the prevailing nutritional environment.

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Oxygen effect on batch cultures of Leuconostoc mesenteroides: relationship between oxygen uptake, growth and end-products

Abstract: OATAO is an open access repository that collects the work of some Toulouse researchers and makes it freely available over the web where possible.

Cited by 19 publications

References 16 publications

NAD(P)H regeneration is the key for heterolactic fermentation of hexoses in Oenococcus oeni

NAD(P)H regeneration is the key for heterolactic fermentation of hexoses in Oenococcus oeni

Growth and energetics of Leuconostoc mesenteroides NRRL B-1299 during metabolism of various sugars and their consequences for dextransucrase production

Fructose and glucose mediates enterotoxin production and anaerobic metabolism ofBacillus cereusATCC14579^T

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Oxygen effect on batch cultures of Leuconostoc mesenteroides: relationship between oxygen uptake, growth and end-products

Abstract: OATAO is an open access repository that collects the work of some Toulouse researchers and makes it freely available over the web where possible.

Cited by 19 publications

References 16 publications

NAD(P)H regeneration is the key for heterolactic fermentation of hexoses in Oenococcus oeni

NAD(P)H regeneration is the key for heterolactic fermentation of hexoses in Oenococcus oeni

Growth and energetics of Leuconostoc mesenteroides NRRL B-1299 during metabolism of various sugars and their consequences for dextransucrase production

Fructose and glucose mediates enterotoxin production and anaerobic metabolism ofBacillus cereusATCC14579T

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Fructose and glucose mediates enterotoxin production and anaerobic metabolism ofBacillus cereusATCC14579^T