Oxygen-generating microparticles downregulate HIF-1α expression, increase cardiac contractility, and mitigate ischemic injury

Mandal, Kalpana; Sangabathuni, Sivakoti; Haghniaz, Reihaneh; Kawakita, Satoru; Mecwan, Marvin; Nakayama, Aya; Zhang, Cher; Edalati, Masoud; Huang, Wei-Ching; Lopez, Ana Hernandez; Jucaud, Vadim; Dokmeci, Mehmet R.; Khademhosseini, Ali

doi:10.1016/j.actbio.2023.01.030

Cited by 9 publications

(7 citation statements)

References 66 publications

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“…The media solution with Presto Blue was transferred to 96‐well plates, and fluorescence was measured using a plate reader at wavelengths of 560 nm and 590 nm (excitation and emission, respectively). The results of assay were expressed as the percentage of viable cells in comparison to the controls taken as 100 % [38] . Cytotoxicity studies of compounds against three cell lines were initially tested at the 50 μM concentration in triplicates.…”

Section: Resultsmentioning

confidence: 99%

“…The staining process involved incubating the spheroids with 2 μM ethidium homodimer‐1 and 2 μM calcein‐AM (diluted in PBS) for 20 minutes at room temperature in the absence of light. The stained cells were then observed with a confocal microscope (Carl Zeiss) featuring Calcein AM (ex 485 nM, em 530 nM) and ethidium bromide (EthD‐1) fluorescence filters (excitation wavelength 530 nM, emission wavelength 645 nm) via objectives ranging from 4X to 40X [38,40] …”

Section: Resultsmentioning

confidence: 99%

“…The results of assay were expressed as the percentage of viable cells in comparison to the controls taken as 100 %. [38] Cytotoxicity studies of compounds against three cell lines were initially tested at the 50 μM concentration in triplicates. The compounds which showed encouraging anticancer activity against each tested cell lines were further screened for cytotoxicity at different concentrations viz., 3.12 μM, 6.25 μM, 12.5 μM, 25 μM, 50 μM, 100 μM, including the positive control gemcitabine.…”

Section: Anticancer Activitymentioning

confidence: 99%

“…The stained cells were then observed with a confocal microscope (Carl Zeiss) featuring Calcein AM (ex 485 nM, em 530 nM) and ethidium bromide (EthD-1) fluorescence filters (excitation wavelength 530 nM, emission wavelength 645 nm) via objectives ranging from 4X to 40X. [38,40]…”

Section: Anticancer Activity In 3d Spheroid Live/dead Assaymentioning

confidence: 99%

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Design, Synthesis, Anticancer and Antimicrobial Studies of 2‐Phenylthiazolidin‐4‐one Glycinamide Conjugates

Singh,

Aggarwal,

Patel

et al. 2023

ChemistrySelect

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Thiazolidin‐4‐one is a versatile nucleus that has been reported to exhibit a diverse array of biological activities, including anticancer and antimicrobial activities. In the current research study, a series of C2‐(p‐substituted phenyl)‐thiazolidin‐4‐one compounds conjugated with anilines were designed and tested for drug‐likeness behaviour. Subsequently, the designed compounds were synthesized, characterized, and tested for anticancer activity against three cancer cell lines, namely liver (HEP−G2 cells), breast (MDA‐MB‐231), and glioblastoma (U87MG cells), with gemcitabine as the reference positive control drug. Initially, the compounds were tested at 50 micromolar, while the MIC was determined for the selected active compounds. Among the three cell lines, MDA‐MB‐231 showed relatively higher sensitivity towards the tested compounds. Four compounds of the series exhibited comparable or superior cytotoxicity to the reference drug gemcitabine. Particularly, N‐(p‐tolyl)‐2‐(2‐(4‐methoxyphenyl)‐4‐oxothiazolidin‐3‐yl)acetamide (6 d) displayed highest cytotoxicity among the series. The anticancer activity of the selected compounds was also tested against 3D spheroid models, in which compound N‐(4‐chlorophenyl)‐2‐(2‐(4‐methoxyphenyl)‐4‐oxothiazolidin‐3‐yl)acetamide (6 a) induced apoptosis in brain tumour spheroids (MTS). Docking studies of compound 6 d were performed against different targets, namely dihydrofolate reductase, phosphoinositide 3‐kinase, epidermal growth factor receptor, dihydroorotate dehydrogenase, and murine double minute 2, which illustrated inhibition of dihydroorotate dehydrogenase as a possible mechanism of toxicity. The compounds were also evaluated in vitro for antibacterial activity against two bacterial strains (E. coli and P. aeruginosa) and a Candida albicans fungal strain.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Anticancer Activitymentioning

confidence: 99%

Section: Anticancer Activity In 3d Spheroid Live/dead Assaymentioning

confidence: 99%

See 2 more Smart Citations

Design, Synthesis, Anticancer and Antimicrobial Studies of 2‐Phenylthiazolidin‐4‐one Glycinamide Conjugates

Singh,

Aggarwal,

Patel

et al. 2023

ChemistrySelect

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“…The developed MPs released O 2 for up to 24 h in a sustained manner and promoted the metabolic functions of neonatal cardiac myocytes in hypoxic conditions, restored the reduced energy of hypoxic cells, and improved cardiac contractility. These oxygen-releasing hydrogels also alleviated MI-related cardiac stress in rabbit heart tissues ex vivo [ 29 ].…”

Section: Oxygenated Scaffolds To Enhance Cell Survival In Vitromentioning

confidence: 99%

Insight on Oxygen-Supplying Biomaterials Used to Enhance Cell Survival, Retention, and Engraftment for Tissue Repair

et al. 2023

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Oxygen is one of the essential requirements for cell survival, retention, and proliferation. The field of regenerative medicine and tissue engineering (TE) has realized considerable achievements for the regeneration of tissues. However, tissue regeneration still lacks the full functionality of solid organ implantations; limited cell survival and retention due to oxidative stress and hypoxia in the deeper parts of tissues remains a perpetual challenge. Especially prior to neovascularization, hypoxia is a major limiting factor, since oxygen delivery becomes crucial for cell survival throughout the tissue-engineered construct. Oxygen diffusion is generally limited in the range 100–200 μm of the thickness of a scaffold, and the cells located beyond this distance face oxygen deprivation, which ultimately leads to hypoxia. Furthermore, before achieving functional anastomosis, implanted tissues will be depleted of oxygen, resulting in hypoxia (<5% dissolved oxygen) followed by anoxic (<0.5% dissolved oxygen) microenvironments. Different types of approaches have been adopted to establish a sustained oxygen supply both in vitro and in vivo. In this review, we have summarized the recent developments in oxygen-generating and/or releasing biomaterials for enhancing cell survival in vitro, as well as for promoting soft and hard tissue repair, including skin, heart, nerve, pancreas, muscle, and bone tissues in vivo. In addition, redox-scavenging biomaterials and oxygenated scaffolds have also been highlighted. The surveyed results have shown significant promise in oxygen-producing biomaterials and oxygen carriers for enhancing cell functionality for regenerative medicine and TE applications. Taken together, this review provides a detailed overview of newer approaches and technologies for oxygen production, as well as their applications for bio-related disciplines.

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Engineering Synthetic Erythrocytes as Next‐Generation Blood Substitutes

Gomes,

Jeong,

Shin

et al. 2024

Adv Funct Materials

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Blood scarcity is one of the main causes of healthcare disruptions worldwide, with blood shortages occurring at an alarming rate. Over the last decades, blood substitutes have aimed at reinforcing the supply of blood, with several products (e.g., hemoglobin (Hb)‐based oxygen (O2) carriers, perfluorocarbons (PFC)) achieving a limited degree of success. Regardless, there is still no widespread solution to this problem due to persistent challenges in product safety and scalability. In this Review, different advances are described in the field of blood substitution, particularly in the development of artificial red blood cells, otherwise known as engineered erythrocytes (EE). The different strategies are categorized into natural, synthetic, or hybrid approaches, and discuss their potential in terms of safety and scalability. Synthetic EEs are identified as the most powerful approach, and describe erythrocytes from a materials engineering perspective. Their biological structure and function are reviewed, as well as explore different methods of assembling a material‐based cell. Specifically, it is discussed how to recreate size, shape, and deformability through particle fabrication, and how to recreate the functional machinery through synthetic biology and nanotechnology. It is concluded by describing the versatile nature of synthetic erythrocytes in medicine and pharmaceuticals and propose specific directions for the field of erythrocyte engineering.

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Oxygen-generating microparticles downregulate HIF-1α expression, increase cardiac contractility, and mitigate ischemic injury

Cited by 9 publications

References 66 publications

Design, Synthesis, Anticancer and Antimicrobial Studies of 2‐Phenylthiazolidin‐4‐one Glycinamide Conjugates

Design, Synthesis, Anticancer and Antimicrobial Studies of 2‐Phenylthiazolidin‐4‐one Glycinamide Conjugates

Insight on Oxygen-Supplying Biomaterials Used to Enhance Cell Survival, Retention, and Engraftment for Tissue Repair

Engineering Synthetic Erythrocytes as Next‐Generation Blood Substitutes

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