Oxygen isotope enrichment (Δ18O) as a measure of time-averaged transpiration rate

Sheshshayee, M. S.; Bindumadhava, H.; Ramesh, R.; Prasad, T. G.; Lakshminarayana, M.; Udayakumar, M.

doi:10.1093/jxb/eri300

Cited by 82 publications

(83 citation statements)

References 37 publications

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“…To test for physiological parameters of rice lines under water-limited conditions, gravimetric analysis was done to measure WUE (14). Rice seedlings of T 3 generation lines, grown in small peat pots for 2 weeks in the greenhouse at 27°C and 60-65% relative humidity, were transplanted into 5-liter pots with soil:sand:peat (1:1:1) of known weight, and the soil surface was covered with a small stone mulch to restrict direct evaporation from the soil surface.…”

Section: Methodsmentioning

confidence: 99%

“…The measurements were made at an ambient CO 2 concentration of 360 mol mol Ϫ1 and photosynthetic photon flux density of 1,800 mol m Ϫ2 s Ϫ1 by using LICOR light source and a chamber temperature of 28°C Ϯ 0.5. Photosynthetic gas exchange and chlorophyll-a fluorescence were measured simultaneously (14 For leaf structure analysis, cross-sections of the third fully expanded leaf from the top of the main tiller from 6-week-old HRD overexpressors and WT plants were made by free-hand sections with a razor blade and then observed for chlorophyll autofluorescence under ϫ40 magnification by using a UV fluorescence microscope (Zeiss, Augsburg, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…In gravimetric estimations of WUE (14), the rice HRD lines revealed a significant 50-100% increase under well watered control conditions and an Ϸ50% increase under drought stress (Fig. 5A).…”

Section: Hrd Overexpression In Rice Improves Drought Resistance and Wuementioning

confidence: 97%

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Improvement of water use efficiency in rice by expression of HARDY , an Arabidopsis drought and salt tolerance gene

Karaba

Dixit

Greco

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

440

251

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Freshwater is a limited and dwindling global resource; therefore, efficient water use is required for food crops that have high water demands, such as rice, or for the production of sustainable energy biomass. We show here that expression of the Arabidopsis HARDY (HRD) gene in rice improves water use efficiency, the ratio of biomass produced to the water used, by enhancing photosynthetic assimilation and reducing transpiration. These drought-tolerant, low-water-consuming rice plants exhibit increased shoot biomass under well irrigated conditions and an adaptive increase in root biomass under drought stress. The HRD gene, an AP2/ERF-like transcription factor, identified by a gain-of-function Arabidopsis mutant hrd-D having roots with enhanced strength, branching, and cortical cells, exhibits drought resistance and salt tolerance, accompanied by an enhancement in the expression of abiotic stress associated genes. HRD overexpression in Arabidopsis produces thicker leaves with more chloroplast-bearing mesophyll cells, and in rice, there is an increase in leaf biomass and bundle sheath cells that probably contributes to the enhanced photosynthesis assimilation and efficiency. The results exemplify application of a gene identified from the model plant Arabidopsis for the improvement of water use efficiency coincident with drought resistance in the crop plant rice.biomass ͉ bundle sheath ͉ photosynthesis ͉ root strength ͉ transcription factor

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of water use efficiency in rice by expression of HARDY , an Arabidopsis drought and salt tolerance gene

Karaba

Dixit

Greco

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

440

251

View full text Add to dashboard Cite

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“…A gravimetric approach was adapted to create water limitation to study the effect of NPC4 alteration on stomatal regulation (Sheshshayee et al, 2005). Arabidopsis seeds were sown in potting soil and stratified for 2 d and then grown for 10 d under 12 h light/12 h dark.…”

Section: Plant Growth and Treatmentsmentioning

confidence: 99%

Nonspecific Phospholipase C NPC4 Promotes Responses to Abscisic Acid and Tolerance to Hyperosmotic Stress inArabidopsis

et al. 2010

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Diacyglycerol (DAG) is an important class of cellular lipid messengers, but its function in plants remains elusive. Here, we show that knockout of the Arabidopsis thaliana nonspecific phospholipase C (NPC4) results in a decrease in DAG levels and compromises plant response to abscisic acid (ABA) and hyperosmotic stresses. NPC4 hydrolyzes various phospholipids in a calcium-independent manner, producing DAG and a phosphorylated head group. NPC4 knockout (KO) plants display decreased ABA sensitivity in seed germination, root elongation, and stomatal movement and had decreased tolerance to high salinity and water deficiency. Overexpression of NPC4 renders plants more sensitive to ABA and more tolerant to hyperosmotic stress than wild-type plants. Addition of a short-chain DAG or a short-chain phosphatidic acid (PA) restores the ABA response of NPC4-KO to that of the wild type, but the addition of DAG together with a DAG kinase inhibitor does not result in a wild-type phenotype. These data suggest that NPC4-produced DAG is converted to PA and that NPC4 and its derived lipids positively modulate ABA response and promote plant tolerance to drought and salt stresses.

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“…The MTR over the course of the experiment was calculated as the cumulative water transpired divided by the leaf area duration (Sheshshayee et al, 2005):…”

Section: Growth and Transpiration Efficiency Measurementsmentioning

confidence: 99%

Conifers, Angiosperm Trees, and Lianas: Growth, Whole-Plant Water and Nitrogen Use Efficiency, and Stable Isotope Composition (δ 13C and δ 18O) of Seedlings Grown in a Tropical Environment

et al. 2008

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Seedlings of several species of gymnosperm trees, angiosperm trees, and angiosperm lianas were grown under tropical field conditions in the Republic of Panama; physiological processes controlling plant C and water fluxes were assessed across this functionally diverse range of species. Relative growth rate, r, was primarily controlled by the ratio of leaf area to plant mass, of which specific leaf area was a key component. Instantaneous photosynthesis, when expressed on a leaf-mass basis, explained 69% of variation in r (P , 0.0001, n 5 94). Mean r of angiosperms was significantly higher than that of the gymnosperms; within angiosperms, mean r of lianas was higher than that of trees. Whole-plant nitrogen use efficiency was also significantly higher in angiosperm than in gymnosperm species, and was primarily controlled by the rate of photosynthesis for a given amount of leaf nitrogen. Whole-plant water use efficiency, TE c , varied significantly among species, and was primarily controlled by c i /c a , the ratio of intercellular to ambient CO 2 partial pressures during photosynthesis. Instantaneous measurements of c i /c a explained 51% of variation in TE c (P , 0.0001, n 5 94). Whole-plant 13 C discrimination also varied significantly as a function of c i /c a (R 2 5 0.57, P , 0.0001, n 5 94), and was, accordingly, a good predictor of TE c . The 18O enrichment of stem dry matter was primarily controlled by the predicted 18 O enrichment of evaporative sites within leaves (R 2 5 0.61, P , 0.0001, n 5 94), with some residual variation explained by mean transpiration rate. Measurements of carbon and oxygen stable isotope ratios could provide a useful means of parameterizing physiological models of tropical forest trees.Tropical forest ecosystems have been subject to extensive perturbations associated with anthropogenic activity in recent decades, and such perturbations will likely continue into the foreseeable future (Laurance et al., 2004;Wright, 2005). Effective environmental management requires knowledge of how such perturbations impact upon cycling of carbon (C) and water between forest trees and the atmosphere, and how these C and water fluxes relate to plant nutrient status. A sound, mechanistic understanding of the physiological processes that control photosynthesis and transpiration in tropical trees is therefore essential for understanding and managing the human impact upon tropical forests. In this study, we analyzed the physiological controls over growth (the relative rate of C accumulation), nitrogen (N) use efficiency (NUE; the rate of C accumulation for a given N content), water use efficiency (the ratio of whole-plant C gain to water loss), and stable isotope composition (d 13

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Oxygen isotope enrichment (Δ18O) as a measure of time-averaged transpiration rate

Cited by 82 publications

References 37 publications

Improvement of water use efficiency in rice by expression of HARDY , an Arabidopsis drought and salt tolerance gene

Improvement of water use efficiency in rice by expression of HARDY , an Arabidopsis drought and salt tolerance gene

Nonspecific Phospholipase C NPC4 Promotes Responses to Abscisic Acid and Tolerance to Hyperosmotic Stress inArabidopsis

Conifers, Angiosperm Trees, and Lianas: Growth, Whole-Plant Water and Nitrogen Use Efficiency, and Stable Isotope Composition (δ 13C and δ 18O) of Seedlings Grown in a Tropical Environment

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