Oxygen Microscopy by Two‐Photon‐Excited Phosphorescence

Finikova, Olga S.; Lebedev, A. V.; Aprelev, Alexey; Troxler, Thomas; Gao, Feng; Garnacho, Carmen; Muro, Silvia; Hochstrasser, Robin M.; Vinogradov, Sergei A.

doi:10.1002/cphc.200800296

Cited by 227 publications

(308 citation statements)

References 40 publications

Supporting

Mentioning

298

Contrasting

Unclassified

Order By: Relevance

“…From our experience investigating cerebral metabolism in vivo, FAD fluorescence is weak and provides an inadequate signal to noise ratio, likely the result of low FAD concentration in cerebral cortex [10]. Additionally, a spectral conflict exists between NADH and the coumarin molecules of presently-available O2-sensitive contrast agent for 2P microscopy [45,46], which currently prevents simultaneous 2PM measurements of pO2 and NADH in tissue. Deciphering as much metabolic information from intrinsic NADH is therefore a highlycoveted goal for characterizing cerebral metabolism in vivo.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence lifetime microscopy of NADH distinguishes alterations in cerebral metabolism in vivo

Yaseen

Sutin

et al. 2017

Biomed. Opt. Express

View full text Add to dashboard Cite

Evaluating cerebral energy metabolism at microscopic resolution is important for comprehensively understanding healthy brain function and its pathological alterations. Here, we resolve specific alterations in cerebral metabolism in vivo in Sprague Dawley rats utilizing minimally-invasive 2-photon fluorescence lifetime imaging (2P-FLIM) measurements of reduced nicotinamide adenine dinucleotide (NADH) fluorescence. Time-resolved fluorescence lifetime measurements enable distinction of different components contributing to NADH autofluorescence. Ostensibly, these components indicate different enzyme-bound formulations of NADH. We observed distinct variations in the relative proportions of these components before and after pharmacological-induced impairments to several reactions involved in glycolytic and oxidative metabolism. Classification models were developed with the experimental data and used to predict the metabolic impairments induced during separate experiments involving bicuculline-induced seizures. The models consistently predicted that prolonged focal seizure activity results in impaired activity in the electron transport chain, likely the consequence of inadequate oxygen supply. 2P-FLIM observations of cerebral NADH will help advance our understanding of cerebral energetics at a microscopic scale. Such knowledge will aid in our evaluation of healthy and diseased cerebral physiology and guide diagnostic and therapeutic strategies that target cerebral energetics. 2(2), 145-150 (1966). 33. L. K. Klaidman, A. C. Leung, and J. D. Adams, Jr., "High-performance liquid chromatography analysis of oxidized and reduced pyridine dinucleotides in specific brain regions," Anal.

show abstract

Section: Discussionmentioning

confidence: 99%

Fluorescence lifetime microscopy of NADH distinguishes alterations in cerebral metabolism in vivo

Yaseen

Sutin

et al. 2017

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…In order to identify spatial distribution of oxygenation in individual retinal blood vessels, imaging-based methods are required. For example, phosphorescence lifetime imaging can map the oxygen tension in mouse retinal vessels based on oxygen-dependent emission quenching of the injected phosphorescent probe [15,16]. Unfortunately, the need to introduce toxic fluorescent probes into the systemic circulation makes it inappropriate for human uses.…”

Section: Introductionmentioning

confidence: 99%

Retinal oximetry in humans using visible-light optical coherence tomography [Invited]

Chen

Xiao

Nesper

et al. 2017

Biomed. Opt. Express

View full text Add to dashboard Cite

Abstract:We measured hemoglobin oxygen saturation (sO 2 ) in the retinal circulation in healthy humans using visible-light optical coherence tomography (vis-OCT). The measurements showed clear oxygenation differences between central retinal arteries and veins close to the optic nerve head. Spatial variations at different vascular branching levels were also revealed. In addition, we presented theoretical and experimental results to establish that noises in OCT intensity followed Rice distribution. We used this knowledge to retrieve unbiased estimation of true OCT intensity to improve the accuracy of vis-OCT oximetry, which had inherently lower signal-to-nose ratio from human eyes due to safety and comfort limitations. We demonstrated that the new statistical-fitting sampling strategy could reduce the estimation error in sO 2 by three percentage points (pp). The presented work aims to provide a foundation for using vis-OCT to achieve accurate retinal oximetry in clinical settings. G. Garhöfer, and L. Schmetterer, "Retinal oxygen metabolism during normoxia and hyperoxia in healthy subjects," Invest.

show abstract

“…The materials used in optical oxygen sensors are quite established. [1][2][3][4] Most oxygen sensors employ such widespread indicators as ruthenium(II) polypyridyl complexes, [5][6][7] platinum(II) and palladium(II) porphyrins [8][9][10][11] and their analogues. [12][13][14] Other luminescent indicators are less popular which is explained by their inferior photophysical properties (low absorption coefficients and luminescence quantum yields, short luminescence decay times, poor photostability etc.)…”

Section: Introductionmentioning

confidence: 99%

Exceptional Oxygen Sensing Properties of New Blue Light‐Excitable Highly Luminescent Europium(III) and Gadolinium(III) Complexes

Borisov

Fischer

Saf

et al. 2014

Adv Funct Materials

View full text Add to dashboard Cite

New europium(III) and gadolinium(III) complexes bearing 8-hydroxyphenalenone antenna combine efficient absorption in the blue part of the spectrum and strong emission in polymers at room temperature. The Eu(III) complexes show characteristic red luminescence whereas the Gd(III) dyes are strongly phosphorescent. The luminescence quantum yields are about 20% for the Eu(III) complexes and 50% for the Gd(III) dyes. In contrast to most state-of-the-art Eu(III) complexes the new dyes are quenched very efficiently by molecular oxygen. The luminescence decay times of the Gd(III) complexes exceed 1 ms which ensures exceptional sensitivity even in polymers of moderate oxygen permeability. These sensors are particularly suitable for trace oxygen sensing and may be good substitutes for Pd(II) porphyrins. The photophysical and sensing properties can be tuned by varying the nature of the fourth ligand. The narrow-band emission of the Eu(III) allows efficient elimination of the background light and autofluorescence and is also very attractive for use e.g. in multi-analyte sensors. The highly photostable indicators incorporated in nanoparticles are promising for imaging applications. Due to the straightforward preparation and low cost of starting materials the new dyes represent a promising alternative to the state-ofthe-art oxygen indicators particularly for such applications as e.g. food packaging.

show abstract

Oxygen Microscopy by Two‐Photon‐Excited Phosphorescence

Cited by 227 publications

References 40 publications

Fluorescence lifetime microscopy of NADH distinguishes alterations in cerebral metabolism in vivo

Fluorescence lifetime microscopy of NADH distinguishes alterations in cerebral metabolism in vivo

Retinal oximetry in humans using visible-light optical coherence tomography [Invited]

Exceptional Oxygen Sensing Properties of New Blue Light‐Excitable Highly Luminescent Europium(III) and Gadolinium(III) Complexes

Contact Info

Product

Resources

About