Oxygen radicals production and actin filament disruption in bivalve haemocytes treated with benzo(a)pyrene

Gómez-Mendikute, Amagoia; Etxeberría, Ainhoa; Olabarrieta, I.; Cajaraville, Miren P.

doi:10.1016/s0141-1136(02)00177-0

Cited by 81 publications

(39 citation statements)

References 9 publications

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“…In addition, the lost of amoeboid shape of haemocytes from T. philippinarum may be a consequence of ROS-mediated alterations in cytoskeleton organisation. Gómez-Mendikute et al (2002) found that oxidative stress mediated by increased levels of ROS (O 2 -, in particular) causes modifications in the cytoskeletal proteins of haemocytes from Mytilus galloprovincialis exposed in vitro to PAHs, provoking the disorganisation of the cytoskeleton. In this regard, it is important to emphasise that a significant inhibition of SOD activity was recorded in haemocytes from NP-exposed clams, suggesting that cells were unable to eliminate O 2 -.…”

Section: Discussionmentioning

confidence: 99%

“…The cationic probe, NR (Merck), was used to evaluate haemocyte membrane stability after NP exposure. Alterations in its uptake are commonly recognised as an indicator of cell damage in in vitro studies (Gómez-Mendikute et al 2002, Matozzo et al 2002. Haemolymph (final volume = 1 ml) from both NP-treated and untreated clams (controls) was centrifuged at 780 × g for 10 min, haemocytes were resuspended in an equal volume of 8 mg l -1 NR dye solution in filtered seawater (FSW) and then incubated at 25°C for 30 min.…”

Section: Animalsmentioning

confidence: 99%

“…Alterations in immunocompetence have been reported for bivalves after exposure, both in vitro and in vivo, to organic and inorganic contaminants, such as heavy metals (Cheng & Sullivan 1984, Cheng 1988, Viarengo et al 1994, Pipe et al 1999, Matozzo et al 2001, Sauvé et al 2002, organotins (Fisher et al 1990, Auffret & Oubella 1997, Cima et al 1998, Matozzo et al 2002, fungicides (Alvarez & Friedl 1992), polycyclic aromatic hydrocarbons (PAHs) (Sami et al 1992, Coles et al 1994, Grundy et al 1996, Gómez-Mendikute et al 2002 and chlorinated phenols (Florence et al 1997). Among the functional responses of bivalve circulating cells, total haemocyte count (THC), viability, motility, aggregation and adhesion capability, phagocytosis, production of reactive oxygen species (ROS), hydrolytic and oxidative enzyme activities, and lysosomal and cell membrane stability are all generally recognised as useful biomarkers in immunotoxicity studies.…”

Section: Introductionmentioning

confidence: 99%

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4-Nonylphenol induces immunomodulation and apoptotic events in the clam Tapes philippinarum

Matozzo¹,

Marin

2005

Mar. Ecol. Prog. Ser.

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The effects of 4-nonylphenol (NP) on functional responses of haemocytes from the clamTapes philippinarum were investigated after 7 d exposure to sublethal NP concentrations (0, 0 + acetone, 0.025, 0.05, 0.1 and 0.2 mg l -1 NP). Haemocytes from both controls and exposed clams were collected, and the effects of NP on uptake of the vital dye Neutral Red (NR), superoxide dismutase (SOD) and lysozyme activities, total haemocyte count (THC) and volume of circulating cells were evaluated. The capability of NP to induce apoptosis was also investigated. Exposure of clams to 0.2 mg l -1 NP significantly increased (p < 0.05) NR uptake when compared with controls, suggesting that NP caused alterations in cell membrane stability. Significant decreases in both SOD and lysozyme activity were observed from 0.05 mg l -1 NP (p < 0.01 and p < 0.05, respectively) with respect to controls, indicating that NP causes oxidative stress and reduces the immunocompetence of the exposed clams. From 0.05 mg l -1 NP, the apoptotic index (percentage of haemocytes showing positivity to the TUNEL reaction) significantly increased (p < 0.001). Apoptotic haemocytes showed shrinkage and lost their amoeboid shape. Moreover, NP exposure significantly increased THC (p < 0.05 at 0.2 mg l -1 NP) and caused a different distribution in size frequency. On the basis of the threshold concentration values, the most sensitive endpoints for NP were SOD, lysozyme and apoptosis assays. Our results highlight a relationship between NP exposure and changes in the functional responses of haemocytes, suggesting that the contaminant induces immunomodulation in T. philippinarum, mainly by altering cell morphology, cell membrane stability and enzymatic activities, and by promoting oxidative stress and apoptotic events.

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Section: Discussionmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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4-Nonylphenol induces immunomodulation and apoptotic events in the clam Tapes philippinarum

Matozzo¹,

Marin

2005

Mar. Ecol. Prog. Ser.

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“…This may be explained by the fact that ROS increased quickly after the BaP stress [28] and a rapid activation of VpGrx1 and VpGrx2 mRNA transcription was required to ensure the synthesis of Grxs. As time progressed on, the ROS induced by BaP stress was eliminated by antioxidants including VpGrx1 and VpGrx2, and then the transcripts decreased to the original level at 96 h. It has been reported the expression of Grx transcript could be induced by pollutant exposure.…”

Section: Temporal Expression Profile Of Vpgrx1 and Vpgrx2 Mrna In Hepmentioning

confidence: 99%

Identification of glutaredoxin 1 and glutaredoxin 2 genes from Venerupis philippinarum and their responses to benzo[a]pyrene and bacterial challenge

Mu¹,

Wang²,

Yuan³

et al. 2012

Fish & Shellfish Immunology

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“…It is a chemical carcinogen and teratogenic agent, and is produced spontaneously by environmental pollution such as traffic clustering and industrial production [11][12][13][14]. The carcinogenesis mechanism of [B(a)P] has been reported to involve DNA adduct formation during initiation stages.…”

Section: Introductionmentioning

confidence: 99%

Effects of Benzo(a)pyrene and Ethanol on Morphology and Antioxidant Status and Transaminases in Rat Liver

Emre¹,

Aktay²,

Polat³

et al. 2014

Med-Science

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Ethanol and benzo (a) benzo(a)pyrene [B(a)P], third group treated with benzo(a)pyrene[B(a)P] plus ethanol (EtOH) and fourth group was given ethanol(EtOH). Superoxide dismutase (SOD), alanin aminotransferase (ALT), aspartat aminotransferase (AST), gamma-glutamyl transferase (GGT), glutathione (GSH), malondialdehyde (MDA) levels as well as histological examination were evaluated to demonstrate the liver response following administration of [B(a)P] and (EtOH

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Oxygen radicals production and actin filament disruption in bivalve haemocytes treated with benzo(a)pyrene

Cited by 81 publications

References 9 publications

4-Nonylphenol induces immunomodulation and apoptotic events in the clam Tapes philippinarum

4-Nonylphenol induces immunomodulation and apoptotic events in the clam Tapes philippinarum

Identification of glutaredoxin 1 and glutaredoxin 2 genes from Venerupis philippinarum and their responses to benzo[a]pyrene and bacterial challenge

Effects of Benzo(a)pyrene and Ethanol on Morphology and Antioxidant Status and Transaminases in Rat Liver

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