Oxygen-releasing biomaterials for regenerative medicine

Wang, Zhaojun; Chen, Tianao; Li, Xin; Guo, Buyun; Liu, Peng; Zhu, Zhiqiang; Xu, Ronald X.

doi:10.1039/d3tb00670k

Cited by 14 publications

(3 citation statements)

References 148 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The objective of this field of research is to develop alternative products or technologies capable of replacing human blood in blood transfusions and an alternative means of resuscitation and oxygen delivery. Hemoglobin-based oxygen carriers, perfluorocarbon-based oxygen carriers, and synthetic blood components have been developed, and the application of stem cell-derived red blood cells (RBCs) has been explored ( 4 , 5 ). Alternatively, the use of animal-derived RBCs could expand the available blood supply.…”

Section: Introductionmentioning

confidence: 99%

Biological response of nonhuman primates to controlled levels of acute blood loss

Roh,

Park,

Lee

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

IntroductionThe global shortage of human blood for medical use has prompted the development of alternative blood sources. Nonhuman primates (NHPs) are commonly used owing to their physiological similarities to humans. The objective of the current study was to establish a controlled-blood-loss model in NHPs to explore their clinical and biological responses.MethodsBlood was sequentially withdrawn from 10 cynomolgus monkeys (10, 14, 18, 22, and 25% of the total blood volume); their vital signs were monitored, and blood parameters were serially analyzed. Humoral mediators in the blood were measured using flow cytometry and enzyme-linked immunosorbent assays.ResultsIn NHPs subjects to 25% blood loss and presenting with related clinical symptoms, the systolic blood pressure ratio on day 0 after bleeding was significantly lower than that of the animals from the other groups (median: 0.65 vs. 0.88, P = 0.0444). Red blood cell counts from day 0–14 and hematocrit levels from day 0–7 were markedly decreased relative to the baseline (P < 0.01). These parameters showed a direct correlation with the extent of blood loss. The levels of creatine phosphokinase, aspartate aminotransferase, and alanine aminotransferase exhibited increases in response to blood loss and had a stronger correlation with the hemoglobin ratio than the volume of blood loss. The levels of C3a and C4a, as well as interleukin (IL)-1α and IL-15, displayed a strong correlation, with no apparent association with blood loss.ConclusionThe findings of the present study showed that only NHPs with 25% blood loss exhibited clinical decompensation and significant systolic blood pressure reduction without fatalities, suggesting that this level of blood loss is suitable for evaluating blood transfusion efficacy or other treatments in NHP models. In addition, the ratio of hemoglobin may serve as a more dependable marker for predicting clinical status than the actual volume of blood loss. Thus, our study could serve as a basis for future xenotransfusion research and to predict biological responses to massive blood loss in humans where controlled experiments cannot be ethically performed.

show abstract

Section: Introductionmentioning

confidence: 99%

Biological response of nonhuman primates to controlled levels of acute blood loss

Roh,

Park,

Lee

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…Currently, hyperbaric oxygen therapy is commonly utilised in clinic, but it has certain contraindications and side effects, that is, oxygen toxicity and barotrauma [13,14]. Additionally, the locally oxygen-supplying biomaterials are developed, that is, peroxides and perfluorocarbons [15,16]. Nevertheless, the primary deficiency of these oxygen-supplying biomaterials is difficult to control the long-term release of O 2 .…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic co‐culture system of algae and human umbilical vein endothelial cells: The effect on alleviating hypoxia and hypoxia/reoxygenation injury

Lin,

Chen,

Tao

et al. 2024

Biosurface and Biotribology

View full text Add to dashboard Cite

It is a developed photosynthetic co‐culture system to alleviate the hypoxia and hypoxia/reoxygenation (H/R)‐injured human umbilical vein endothelial cells (HUVECs). The algae, Chlorella vulgaris, were encapsulated to slow their growth while not affecting the photosynthetic oxygen‐producing capacity by Layer‐by‐layer (LbL) using gelatin and sodium alginate as the positive and negative charges materials, respectively. Then, the photosynthetic co‐culture system of HUVECs and self‐oxygenating alginate hydrogel (Algae‐gel) was constructed in which the optimal ratios between algae and HUVECs were 5:1 and 20:1 for a 2D or 3D co‐cultured manner, respectively. It indicated that the 3D co‐cultured manner of HUVECs needed more O2 by the production of algae than it did in a 2D co‐cultured manner. The co‐cultured Algae‐gel could alleviate hypoxia and the oxidative stress injury of hypoxia and hypoxia/reoxygenation (H/R)‐treated HUVECs in the proliferation, intracellular ROS and cellular migratory ability. In addition, the Algae‐gel could downregulate the expression of hypoxia‐inducible factors 1α (HIF‐1α) and vascular endothelial growth factor (VEGF) of hypoxia and H/R‐injured HUVECs due to the improvement of hypoxia and H/R injury. This photosynthetic co‐culture system could offer a promising approach for repairing hypoxia and H/R‐injured cells or tissue by providing safe and stable O2.

show abstract

“…However, their utility is limited by off-target toxicities, systemic inflammatory changes, and low monotherapy efficacy (21,(23)(24)(25)(26). Recently, innovative O 2 -generating biomaterials have emerged as a promising approach for modulating hypoxic microenvironments (27)(28)(29)(30)(31)(32)(33)(34)(35)(36). Specifically, our lab has recently reported on injectable and macroporous O 2 -cryogels, composed of calcium peroxide (CaO 2 ) particles and acrylate-PEGcatalase (APC), and fabricated with methacrylated hyaluronic acid (HAGM) via cryopolymerization (37)(38)(39)(40).…”

Section: Introductionmentioning

confidence: 99%

Biomaterial-assisted local oxygenation safeguards the prostimulatory phenotype and functions of human dendritic cells in hypoxia

Bhatt,

Nukovic,

Colombani

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

Dendritic cells (DCs), professional antigen-presenting cells, function as sentinels of the immune system. DCs initiate and fine-tune adaptive immune responses by presenting antigenic peptides to B and T lymphocytes to mount an effective immune response against cancer and pathogens. However, hypoxia, a condition characterized by low oxygen (O2) tension in different tissues, significantly impacts DC functions, including antigen uptake, activation and maturation, migration, as well as T-cell priming and proliferation. In this study, we employed O2-releasing biomaterials (O2-cryogels) to study the effect of localized O2 supply on human DC phenotype and functions. Our results indicate that O2-cryogels effectively mitigate DC exposure to hypoxia under hypoxic conditions. Additionally, O2-cryogels counteract hypoxia-induced inhibition of antigen uptake and migratory activity in DCs through O2 release and hyaluronic acid (HA) mediated mechanisms. Furthermore, O2-cryogels preserve and restore DC maturation and co-stimulation markers, including HLA-DR, CD86, and CD40, along with the secretion of proinflammatory cytokines in hypoxic conditions. Finally, our findings demonstrate that the supplemental O2 released from the cryogels preserves DC-mediated T-cell priming, ultimately leading to the activation and proliferation of allogeneic CD3+ T cells. This work emphasizes the potential of local oxygenation as a powerful immunomodulatory agent to improve DC activation and functions in hypoxia, offering new approaches for cancer and infectious disease treatments.

show abstract

Oxygen-releasing biomaterials for regenerative medicine

Abstract: Oxygen is critical to the survival, function and fate of mammalian cells. Oxygen tension controls cellular behavior through metabolic programming, which in turn controls tissue regeneration. A variety of biomaterials...

Cited by 14 publications

References 148 publications

Biological response of nonhuman primates to controlled levels of acute blood loss

Biological response of nonhuman primates to controlled levels of acute blood loss

Photosynthetic co‐culture system of algae and human umbilical vein endothelial cells: The effect on alleviating hypoxia and hypoxia/reoxygenation injury

Biomaterial-assisted local oxygenation safeguards the prostimulatory phenotype and functions of human dendritic cells in hypoxia

Contact Info

Product

Resources

About