Oxygen Transfer and Fragmentation of<i>Aspergillus niger</i>Pellets in Stirred Tank and Concentric-Duct Airlift Bioreactors

Buffo, Mariane Molina; Esperança, Mateus N.; Béttega, Rodrigo; Farinas, Cristiane S.; Badino, Alberto Colli

doi:10.1089/ind.2020.29199.mmb

Cited by 10 publications

(6 citation statements)

References 24 publications

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“…In the literature, few studies take the influence of the increased viscosity of cultivation broths on the particle stress into account. Buffo et al [36] conducted fragmentation experiments with A. niger in a conventional bioreactor with different impellers using a 0.15 wt % xanthan gum solution as the continuous phase of the final diluted suspension. Pilz and Hempel [37] conducted experiments with a similar clay flocculation system as in the current study to investigate the particle stress in airlift loop reactors and bubble columns.…”

Section: Influence Of Fluid Rheology On Particle Stressmentioning

confidence: 99%

Comparative Study of Fluid Dynamic Stress on Aspergillus niger and Model Systems

Waldherr,

Bliatsiou,

Böhm

et al. 2024

Chemie Ingenieur Technik

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The productivity of Aspergillus niger cultivations is strongly linked to the pellet morphology. In this article, the agitation‐ and aeration‐induced stress on bio agglomerates is studied. To facilitate investigations, non‐biological particle systems are extensively used to model the actual biological matter. The literature lacks a direct comparison of model systems with the biological system of interest, leaving uncertainties in the applicability of such systems. This work shows that the results for the impellers' stress intensity of three A. niger strains agree qualitatively with the ones of four developed model systems of different nature (s/l, l/l). The findings justify the usage of model systems, despite certain limitations originating from their specific characteristic properties.

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Section: Influence Of Fluid Rheology On Particle Stressmentioning

confidence: 99%

Comparative Study of Fluid Dynamic Stress on Aspergillus niger and Model Systems

Waldherr,

Bliatsiou,

Böhm

et al. 2024

Chemie Ingenieur Technik

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“…Usually, bioreactors are first characterized abiotically (i.e., in the absence of cells) before a biological characterization is performed during cultivation. The following methods are the most common: abiotic saturation‐based methods (gassing‐out method and dynamic sulfite method) 103, 104, measurement with chemical model system (static sulfite method, hydrazine method, glucose oxidase method) 13, biotic saturation‐based methods (respiratory gassing‐out method) 102, biotic steady‐state methods (gas balancing method) 12. …”

Section: Experimental Approachesmentioning

confidence: 99%

Oxygen Mass Transfer in Biopharmaceutical Processes: Numerical and Experimental Approaches

Seidel

Maschke

Werner

et al. 2020

Chemie Ingenieur Technik

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This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Dedicated to Prof. Dr.-Ing. Matthias Kraume on the occasion of his 65th birthday Oxygen supply in aerobic bioprocesses is of crucial importance. For this reason, this paper presents the oxygen demand of different cells and summarizes experimental and numerical possibilities for the determination of oxygen transfer in bioreactors. The focus lies on the volumetric oxygen mass transfer coefficient (k L a) calculation using computational fluid dynamics and state-of-the-art models for surface-aerated and forced-aerated bioreactors. In addition, experimental methods for the determination of the k L a value and the gas bubble size distribution are presented.

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“…An airlift bioreactor is a gas–liquid bioreactor or a bubble column with a draft tube to circulate the fluid in the bioreactor. However, an airlift bioreactor is more widely used than a bubble column in chemical and biochemical processes because of its beneficial advantages such as better mixing and higher capacity handling. , Advantages of this bioreactor type include a reduction of cost associated with agitation and aeration, ease of scale-up, and low shear force. , Compared with a conventional stirred bioreactor, an airlift bioreactor shows advantages in specific power consumption and mass transfer efficiency . Therefore, an airlift-type bioreactor is suggested to cultivate microorganisms, particularly shear-sensitive cells in a viscous medium, because of a low shear environment and a reasonable oxygen transfer rate with a low power consumption .…”

Section: Introductionmentioning

confidence: 99%

“…33,34 Compared with a conventional stirred bioreactor, an airlift bioreactor shows advantages in specific power consumption and mass transfer efficiency. 35 Therefore, an airlift-type bioreactor is suggested to cultivate microorganisms, particularly shearsensitive cells in a viscous medium, because of a low shear environment and a reasonable oxygen transfer rate with a low power consumption. 36 Moreover, it is easy to scale up the process.…”

Section: Introductionmentioning

confidence: 99%

Alginate Production of Azotobacter vinelandii Using Sugar Cane Juice as the Main Carbon Source in an Airlift Bioreactor

Chuacharoen

Aroonsong

Chysirichote

2022

Ind. Eng. Chem. Res.

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Bacterial alginate production has been studied to solve the uncontrollable problem of natural algae alginate composition. A challenge in using alginate in widespread applications is the high cost of equipment and operation. In this research, we aimed to optimize the conditions to produce alginate by Azotobacter vinelandii ATCC 9046 using sugar cane juice as the main carbon source. A 5-L internal circulating airlift bioreactor was designed and constructed for this research to ensure low installation and operation costs. An equivalent 60.0 mM sucrose concentration optimized for alginate production in this research was used to prepare the sugar cane juice medium for studying the effects of the nitrogen content and the pH value of the culture for alginate fermentation. The results showed that the pH-controlled value and the nitrogen concentration affected the alginate production and that the pH value of the culture affected the molecular weight of obtained alginate. A regression model was developed to predict the alginate production with a good acceptance (R 2 = 81.3%). Moreover, the highest alginate production and its molecular weight obtained from the sugar cane juice medium fermented in the air lift bioreactor were as high as 7.29 ± 0.07 g L −1 and 4,735 kDa, respectively.

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Oxygen Transfer and Fragmentation ofAspergillus nigerPellets in Stirred Tank and Concentric-Duct Airlift Bioreactors

Cited by 10 publications

References 24 publications

Comparative Study of Fluid Dynamic Stress on Aspergillus niger and Model Systems

Comparative Study of Fluid Dynamic Stress on Aspergillus niger and Model Systems

Oxygen Mass Transfer in Biopharmaceutical Processes: Numerical and Experimental Approaches

Alginate Production of Azotobacter vinelandii Using Sugar Cane Juice as the Main Carbon Source in an Airlift Bioreactor

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