Oxygen transfer and gas holdup in airlift bioreactors assembled with helical flow promoters

Picão, Bruno Willian; Gonçalves, Daniele O.; Ribeiro, Renata M M G P; Esperança, Mateus N.; Peixoto, Guilherme; Cerri, Marcel O.

doi:10.1007/s00449-023-02853-w

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…The production of carotenoids by aerobic R. toruloides is influenced not only by agitation speed but also by the dissolved oxygen level. These parameters are crucial for determining the efficiency of oxygen transfer and are widely employed as aeration and agitation controls in aerobic processes [31]. To analyze the impact of the initial oxygen transfer rate (OTR), the same conditions used for evaluating L a were applied.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effects of Stirring and Aeration Rate on Carotenoid Production in Yeast Rhodotorula toruloides CCT 7815 Envisioning Their Application as Soap Additives

Ribeiro,

Picão,

Gonçalves

et al. 2023

Fermentation

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The production of carotenoids by microbial organisms has gained significant interest due to the growing demand for natural products. Among the non-model oleaginous red yeasts, Rhodotorula toruloides stands out as an appealing host for natural carotenoid production. R. toruloides possesses the natural ability to metabolize a wide range of substrates, including lignocellulosic hydrolysates, and convert them into lipids and carotenoids. In this study, we focused on utilizing xylose, the main component of hemicellulose, as the major substrate for R. toruloides. We conducted a comprehensive kinetic evaluation to examine the impact of aeration and agitation on carotenoid production. Results in stirred-tank reactor demonstrated that under milder conditions (300 rpm and 0.5 vvm), R. toruloides accumulated over 70% of its cell mass as lipids. Furthermore, the highest carotenoid yields were achieved at high agitation rates (700 rpm), with carotenoid levels reaching nearly 120 µg/mL. Several carotenoids were identified, including β-carotene, γ-carotene, torularhodin, and torulene, with β-carotene being the major carotenoid, accounting for up to 70% of the total carotenoid content. The carotenoid-rich extract produced by R. toruloides under evaluated conditions was successfully incorporated into soap formulations, demonstrating the addition of antioxidant properties. This work provides a comprehensive understanding of xylose conversion into natural carotenoids by R. toruloides, presenting a promising avenue for their application in cosmetics. Furthermore, this study highlights the potential of a renewable and cost-effective approach for carotenoid production in the soap industry.

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Section: Discussionmentioning

confidence: 99%

Synergistic Effects of Stirring and Aeration Rate on Carotenoid Production in Yeast Rhodotorula toruloides CCT 7815 Envisioning Their Application as Soap Additives

Ribeiro,

Picão,

Gonçalves

et al. 2023

Fermentation

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“…W. Xiao et al [71] found that nanobubble aeration effectively provided extra oxygen for microbial aggregates, achieving a notable improvement in the structural characteristics and performance of microbial aggregates. Furthermore, several studies have applied this technology to different microbial strains, as shown in Table 3, which is attributed to the long lifetime of nanobubbles and their high gas solubility in liquid media [72]. Stimulates the proliferation rate of yeast cells by enhancing their biomass production.…”

Section: Effects On Microbial Activity and Viabilitymentioning

confidence: 99%

Use of Nanobubbles to Improve Mass Transfer in Bioprocesses

Silva,

Arias-Torres,

Carlesi

et al. 2024

Processes

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Nanobubble technology has emerged as a transformative approach in bioprocessing, significantly enhancing mass-transfer efficiency for effective microbial activity. Characterized by their nanometric size and high internal pressure, nanobubbles possess distinct properties such as prolonged stability and minimal rise velocities, allowing them to remain suspended in liquid media for extended periods. These features are particularly beneficial in bioprocesses involving aerobic strains, where they help overcome common obstacles, such as increased culture viscosity and diffusion limitations, that traditionally impede efficient mass transfer. For instance, in an experimental setup, nanobubble aeration achieved 10% higher soluble chemical oxygen demand (sCOD) removal compared to traditional aeration methods. Additionally, nanobubble-aerated systems demonstrated a 55.03% increase in caproic acid concentration when supplemented with air nanobubble water, reaching up to 15.10 g/L. These results underscore the potential of nanobubble technology for optimizing bioprocess efficiency and sustainability. This review delineates the important role of the mass-transfer coefficient (kL) in evaluating these interactions and underscores the significance of nanobubbles in improving bioprocess efficiency. The integration of nanobubble technology in bioprocessing not only improves gas exchange and substrate utilization but also bolsters microbial growth and metabolic performance. The potential of nanobubble technology to improve the mass-transfer efficiency in biotechnological applications is supported by emerging research. However, to fully leverage these benefits, it is essential to conduct further empirical studies to specifically assess their impacts on bioprocess efficacy and scalability. Such research will provide the necessary data to validate the practical applications of nanobubbles and identify any limitations that need to be addressed in industrial settings.

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“…The particle fluidization capacity was enhanced by the presence of intense liquid rotational motion at the bottom of the ILALR with HFPs. Rasanen et al 39 and Picaõ et al 40 compared the hydrodynamics and mass transfer rate in ILALRs with and without HFPs. It was confirmed that the gas holdup and k L a increased in the ILALRs with HFPs.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Bubbly Flow, Mass Transfer, and CO₂ Absorption in a Two-Stage Internal Loop Airlift Reactor Assembly with a Diamond-Shaped Guide Baffle

Song,

Zheng,

Guo

et al. 2024

Ind. Eng. Chem. Res.

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Airlift reactors are one of the most commonly used gas–liquid reactors in industry. The bubble flow regime, gas holdup, and bubble size are vital for mass transfer and chemical reaction in an airlift reactor. To improve the reactor performance, this study suggests a two-stage internal loop airlift reactor assembly with a diamond-shaped guide baffle. The hydrodynamic parameters, volumetric mass transfer coefficient, and the CO2 removal efficiency in the airlift reactor are measured. The effect of the guide baffle on the bubble flow regime, bubble size, gas holdup, mass transfer coefficient, and CO2 removal efficiency is investigated. Compared with a regular airlift reactor, the proposed airlift reactor features an increase in global gas holdup and volumetric mass transfer coefficients of up to 31.6 and 87.9%, respectively. Additionally, the CO2 removal efficiency increases in the airlift reactor assembly with the guide baffle.

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Oxygen transfer and gas holdup in airlift bioreactors assembled with helical flow promoters

Cited by 5 publications

References 44 publications

Synergistic Effects of Stirring and Aeration Rate on Carotenoid Production in Yeast Rhodotorula toruloides CCT 7815 Envisioning Their Application as Soap Additives

Synergistic Effects of Stirring and Aeration Rate on Carotenoid Production in Yeast Rhodotorula toruloides CCT 7815 Envisioning Their Application as Soap Additives

Use of Nanobubbles to Improve Mass Transfer in Bioprocesses

Characteristics of Bubbly Flow, Mass Transfer, and CO₂ Absorption in a Two-Stage Internal Loop Airlift Reactor Assembly with a Diamond-Shaped Guide Baffle

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Oxygen transfer and gas holdup in airlift bioreactors assembled with helical flow promoters

Cited by 5 publications

References 44 publications

Synergistic Effects of Stirring and Aeration Rate on Carotenoid Production in Yeast Rhodotorula toruloides CCT 7815 Envisioning Their Application as Soap Additives

Synergistic Effects of Stirring and Aeration Rate on Carotenoid Production in Yeast Rhodotorula toruloides CCT 7815 Envisioning Their Application as Soap Additives

Use of Nanobubbles to Improve Mass Transfer in Bioprocesses

Characteristics of Bubbly Flow, Mass Transfer, and CO2 Absorption in a Two-Stage Internal Loop Airlift Reactor Assembly with a Diamond-Shaped Guide Baffle

Contact Info

Product

Resources

About

Characteristics of Bubbly Flow, Mass Transfer, and CO₂ Absorption in a Two-Stage Internal Loop Airlift Reactor Assembly with a Diamond-Shaped Guide Baffle