Abstract-In this paper, a lab-scale aerated vessel equipped with a surface aeration impeller was developed to examine standard aeration efficiency (SAE) and mixing time. The SAE and mixing specifications were investigated via variations in the operating parameters, such as rotor speed, impeller immersion depth and water height. Surface aeration was performed using a Rushton disk turbine. Results showed that variation of impeller immersion depth has a greater effect on SAE compare to changes in water level. Moreover, SAE increases with rotor speed up to about 150 to 200 rpm and then decrease. In addition, mixing time is reduced with increase in rotor speed. According to the Response surface methodology (RSM) statistical optimization, optimum values of 169 rpm, 25 mm and 30 cm were found for rotor speed, impeller immersion depth and water height respectively in the range of this study to achieve the maximum value of SAE.