Oxygen-triggered mouse oocyte maturation in vitro and lactate utilization by mouse oocytes and zygotes

Gh, Zeilmaker; Wc, Hülsmann; Wensinck, F; Verhamme, Camiel

doi:10.1530/jrf.0.0290151

Cited by 29 publications

(8 citation statements)

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“…However, it is impossible to determine whether the increase in the number of oocytes containing these structures was due to a component of FBS directly affecting their appearance or was an indirect result of the increased viability of the oocytes in FBS-supplemented medium. A requirement for oxygen for the beneficial effect of pyruvate on oocyte maturation has been demonstrated (Haidri et al, 1971;Zeilmaker et al, 1972). The gas phase in the present experiments included 5% 02, so it is possible that oxidative metabolism of pyruvate provides conditions conducive to oocyte maturation that are not present in the absence of this energy source.…”

Section: Discussionmentioning

confidence: 99%

Requirement for glucose in ligand-stimulated meiotic maturation of cumulus cell-enclosed mouse oocytes

Fagbohun¹,

Downs²

1992

Reproduction

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In this study, the effect of different energy sources used in Eagle's minimum essential medium on the meiotic maturation of mouse oocytes in culture was examined. The effects of glucose (5.5 mmol 1(-1)), pyruvate (0.23 mmol 1(-1)) and glutamine (2 mmol 1(-1)) in different combinations were tested on the maturation of denuded oocytes in the presence or absence of 300 mumol dibutyryl cAMP 1(-1) during 17-18 h of culture. In the absence of cyclic nucleotide, only oocytes from those groups containing pyruvate resumed maturation at a high frequency (99-100% germinal vesicle breakdown); all other combinations resulted in < or = 54% germinal vesicle breakdown. When dibutyryl cAMP was introduced, all pyruvate-containing groups exhibited maturation frequencies of about 50%, whereas maturation in all other groups was negligible (< or = 10% GVB). Pyruvate was also important for the maintenance of viability in denuded oocytes (> or = 86% viability in pyruvate-containing medium; < or = 35% viability in pyruvate-free groups). When cumulus cell-enclosed oocytes were cultured in medium without inhibitor, all combinations of energy substrates supported high frequencies of maturation (> or = 89% germinal vesicle breakdown) and viability (> or = 91%). The addition of dibutyryl cAMP resulted in inhibition of meiotic maturation (5-33% germinal vesicle breakdown) in all cultures except the pyruvate-alone group (97% germinal vesicle breakdown). Viability in cumulus cell-enclosed oocytes was greatest when two or more energy substrates were present in the medium. Follicle-stimulating hormone (FSH) produced a stimulation of meiotic maturation in all cultures of meiotically arrested cumulus cell-enclosed oocytes, but maximal induction of germinal vesicle breakdown was dependent upon D-glucose. Concanavalin A (ConA)-induced meiotic maturation was also dependent upon D-glucose. Uptake and metabolism of D-glucose by the cumulus cells is important in mediating the stimulatory effects of these ligands on oocyte maturation because (1) both FSH and ConA stimulated uptake of D-glucose and 2-deoxyglucose but not 3-O-methylglucose; (2) phloretin prevented the stimulatory action of FSH and ConA on germinal vesicle breakdown at a concentration that suppressed ligand-induced uptake of D-glucose; (3) 2-deoxyglucose, a hexose that suppresses glycolysis, prevented the induction of meiotic maturation by FSH and ConA and (4) D-mannose, a glycolysable sugar, was as effective as D-glucose in supporting the ligand effects.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Discussionmentioning

confidence: 99%

Requirement for glucose in ligand-stimulated meiotic maturation of cumulus cell-enclosed mouse oocytes

Fagbohun¹,

Downs²

1992

Reproduction

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“…The possibility should be considered that an increased thecal blood supply after LH exposure may induce a change in follicular oxygen tension leading to utilization of follicular lactate and the aerobic formation of ATP in the oocyte, a prerequisite for maturation (Zeilmaker et al 1972;Zeilmaker 8c Verhamme 1974).…”

Section: Discussionmentioning

confidence: 99%

Lactate Concentrations in Pre-Ovulatory Follicles of Pro-Oestrous Rats Before and After Onset of Oocyte Maturation

Zeilmaker¹,

Verhamme²

1977

Acta Endocrinologica

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Lactate concentrations were determined in pre-ovulatory follicles of rats at pro-oestrus before and after onset of oocyte maturation. It appeared that high concentrations prevail before and after the time of the expected LH surge (27 mM). The level in serum was about 5 mm. Explanted oocytes obtained from pre-puberal rats and surrounded by cumulus cells, matured in the presence of 20 mm. lactate as sole exogenous energy source.It is argued that oxygen may be the limiting factor suppressing oocyte maturation in vivo.It is well known that the LH surge induces completion of oocyte maturation in the pre-ovulatory follicles of the rat ovary (Ayalon et al. 1972; Vermeiden 8c Zeilmaker 1974). At the same time changes in the cellular structure of the cumulus oophorus, a decrease in oxygen consumption of the cumulus oocyte complex and a release of lactate into the medium of incubated follicles have been observed (Dekel et al. 1976;Hillensjö 1976).Incubation of morning-gathered denuded oocytes obtained from pre-puberal and pro-oestrous rats in media containing lactate leads to maturation, including formation of the second polar body (Zeilmaker 8c Verhamme 1976). The question may arise whether an increased lactate production after LH exposure is instrumental in the induction of oocyte maturation in the follicle.With the support of the Dutch Foundation for Medical Research (FUNGO). 380

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“…'Suitable conditions' mean that not only must meiosis resume, but that the cumulus cells should continue to divide and pyknotic nuclei should not appear. Gwatkin Iwamatsu & Yanagimachi, 1975 Plachot Zeilmaker et al, 1972 Thibault & Gérard, 1971 Thibault et al, 1975b The chronology of successive events should also be absolutely similar to that in vivo (Thibault & Gérard, 1971 ;Thibault, Gérard & Menezo, 1975a).…”

Section: Maturation Of Follicle-free Oocytes In Mammalsmentioning

confidence: 96%

Hammond Memorial Lecture

Thibault¹

1977

Reproduction

159

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Oxygen-triggered mouse oocyte maturation in vitro and lactate utilization by mouse oocytes and zygotes

Cited by 29 publications

References 0 publications

Requirement for glucose in ligand-stimulated meiotic maturation of cumulus cell-enclosed mouse oocytes

Requirement for glucose in ligand-stimulated meiotic maturation of cumulus cell-enclosed mouse oocytes

Lactate Concentrations in Pre-Ovulatory Follicles of Pro-Oestrous Rats Before and After Onset of Oocyte Maturation

Hammond Memorial Lecture

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