Oxygen uptake and the potentiating effects of increased hemolymph lactate on oxygen transport during exercise in the blue crab,Callinectes sapidus

Booth, Charles E.; McMahon, Brian R.; Pinder, Alan W.

doi:10.1007/bf00688894

Cited by 143 publications

(56 citation statements)

References 53 publications

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“…In both of these studies, crabs exhibited periods of active vertical swimming separated by periods on the bottom. The swimming durations did not appear to be limited by aerobic swimming capacity, which can exceed 1 h under laboratory conditions (Booth et al 1982), nor did they appear to be associated with feeding in the water column, as blue crabs usually feed on benthic organisms, as indicated by gut content analysis (Tagatz 1968). Using hydrostatic pressure changes as a cue, a crab on the bottom would sense a negative ‫‪t‬ץ/ץ‬ associated with ebb tide.…”

Section: Discussionmentioning

confidence: 99%

Testing a selective tidal‐stream transport model: Observations of female blue crab (Callinectes sapidus) vertical migration during the spawning season

Hench¹,

Forward²,

Carr³

et al. 2004

Limnology & Oceanography

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Female blue crabs, Callinectes sapidus, mate in estuaries and undergo a seaward spawning migration to release larvae. According to the prevailing model, females with mature embryos use nocturnal ebb-tide transport (ETT) to move seaward, release larvae, and then reverse to nocturnal flood-tide transport (FTT) to move back into the estuary. We tested this model by examining the vertical migratory behavior of ovigerous and post-larval release female crabs. Simultaneous physical-biological data were collected for 38 d during Aug 2002-Sep 2002 in Bogue Sound, North Carolina. Crab water-column positions were determined with miniature internally recording pressure sensors. Local current and water properties were measured, and crab vertical migration times relative to observed currents were used to determine ETT and FTT behavior. Surface censuses of free-swimming crabs on 19 nocturnal ebbs were used to complement the intensive studies of individual crabs. The study found that (1) the pressure sensors had a measurable but small effect on swimming, (2) females migrated during day and night ebb tides, (3) females used ETT throughout embryo development, (4) ETT corresponded to the rate of decrease in water level (hydrostatic pressure), (5) larvae were released at high tide or when water level was falling, often within several hours of sunrise, and (6) post-larval release females continued ETT and did not switch to FTT. Thus, the data did not support the prevailing ETT-FTT reversal model. Rather, females continue ETT into coastal areas, releasing subsequent clutches farther seaward, which increases the potential for successful larval transport to favorable offshore developmental areas.For many marine species, successful recruitment entails adult migration out of an estuary and subsequent larval transport into an estuary. While substantial literature is accumulating on the latter process (e.g., Weinstein 1988; Crowder and Werner 1999;Epifanio and Garvine 2001), 1 To whom correspondence should be addressed. Present address: Environmental Fluid Mechanics Laboratory, Stanford University, Stanford, California 94305-24-4020 (jhench@stanford.edu). AcknowledgmentsWe thank Pia Moisander and Josh Osterberg for valuable assistance in the field. Sean Powers and two anonymous reviewers provided helpful suggestions on the manuscript.

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Section: Discussionmentioning

confidence: 99%

Testing a selective tidal‐stream transport model: Observations of female blue crab (Callinectes sapidus) vertical migration during the spawning season

Hench¹,

Forward²,

Carr³

et al. 2004

Limnology & Oceanography

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“…Tagging studies have shown that blue crabs typically move 50-900m within estuaries on a daily basis, but may migrate seasonally over larger distances of up to 800km, using a combination of swimming high in the water column on flood tides and walking along the bottom on ebb tides (Hines, 2007). However, there are currently no data available on the energetics of walking in the blue crab, as studies of exercise physiology in blue crabs have been largely focused on swimming (Booth et al, 1982;Booth and McMahon, 1985).…”

Section: Introductionmentioning

confidence: 99%

“…There is anywhere from a two-to a sevenfold elevation of oxygen uptake during increased activity, made possible by increases in ventilation rate, heart rate and cardiac output (Herreid et al, 1979;McMahon et al, 1979;Wood and Randall, 1981;Booth et al, 1982;Taylor, 1982;Full and Herreid, 1984;Houlihan and Innes, 1984;Houlihan et al, 1985;Hamilton and Houlihan, 1992). Elevated oxygen uptake persists for some time after activity is completed.…”

Section: Introductionmentioning

confidence: 99%

“…Highly aerobic crustaceans, such as the blue crab, typically have a small oxygen deficit recovery (e.g. Booth et al, 1982) and oxygen uptake often returns to preactivity levels within 20-45min (Herreid et al, 1979;Booth et al, 1982;Full and Herreid, 1983;Houlihan and Innes, 1984;Hamilton and Houlihan, 1992). However, in organisms more reliant on anaerobic pathways to fuel energy demands during activity, this recovery may take as long as 5-24h McMahon et al, 1979;Wood and Randall, 1981).…”

Section: Introductionmentioning

confidence: 99%

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Energy metabolism and metabolic depression during exercise inCallinectes sapidus, the Atlantic blue crab: effects of the bacterial pathogenVibrio campbellii

Thibodeaux

Burnett

2009

Journal of Experimental Biology

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SUMMARYCallinectes sapidus (Rathbun), the Atlantic blue crab, commonly harbors low to moderate amounts of bacteria in hemolymph and other tissues. These bacteria are typically dominated by Vibrio spp., which are known to cause mortality in the blue crab. The dose-dependent lethality of an isolate of Vibrio campbellii was determined in crabs; the mean 48h LD 50 (half-maximal lethal dose) was 6.2ϫ10 5 colony forming unitsg -1 crab. Injection of a sublethal dose of V. campbellii into the hemolymph of the crab resulted in a rapid and large depression (30-42%) of metabolic rate, which persisted for 24h. Because gills are an organ of immune function as well as respiration, we were interested in how bacteria injected into the crab would affect the energetic costs associated with walking. Overall metabolism (aerobic and anaerobic) more than doubled in crabs walking for 30min at 8mmin -1 . The metabolic depression resulting from bacterial injection persisted throughout the exercise period and patterns of phosphagen and adenylate consumption within walking leg muscle were not affected by treatment. The ability of crabs to supply required energy for walking is largely unaffected by exposure to Vibrio; however, Vibrio-injected crabs are less aerobic while doing so. This depressed metabolic condition in response to bacteria, present during moderate activity, could be a passive result of mounting an immune response or may indicate an actively regulated metabolic depression. A compromised metabolism can affect the performance of daily activities, such as feeding and predator avoidance or affect the ability to cope with environmental stressors, such as hypoxia.

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“…The hypothesis here was that increased salinity would lead to a reduction in gill perfusion and, consequently, reduced oxygen consumption. Reduced oxygen consumption would result in anaerobic metabolism and lead to increased levels of hemolymph lactate (Booth et al 1982). In many decapod crustaceans, lactate is the main product of metabolism when there is a hypoxic condition (Bridges and Brand 1980, Taylor and Spicer 1989, Maciel et al 2008.…”

Section: Discussionmentioning

confidence: 99%

Oxygen consumption remains stable while ammonia excretion is reduced upon short time exposure to high salinity in Macrobrachium acanthurus (Caridae: Palaemonidae), a recent freshwater colonizer

et al. 2017

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ABSTRACT. Palaemonid shrimps occur in the tropical and temperate regions of South America and the Indo-Pacific, in brackish/freshwater habitats, and marine coastal areas. They form a clade that recently (i.e., ~30 mya) invaded freshwater, and one included genus, Macrobrachium Bate, 1868, is especially successful in limnic habitats. Adult Macrobrachium acanthurus (Wiegmann, 1836) dwell in coastal freshwaters, have diadromous habit, and need brackish water to develop. Thus, they are widely recognized as euryhaline. Here we test how this species responds to a short-term exposure to increased salinity. We hypothesized that abrupt exposure to high salinity would result in reduced gill ventilation/perfusion and decreased oxygen consumption. Shrimps were subjected to control (0 psu) and experimental salinities (10, 20, 30 psu), for four and eight hours (n = 8 in each group). The water in the experimental containers was saturated with oxygen before the beginning of the experiment; aeration was interrupted before placing the shrimp in the experimental container. Dissolved oxygen (DO), ammonia concentration, and pH were measured from the aquaria water, at the start and end of each experiment. After exposure, the shrimp's hemolymph was sampled for lactate and osmolality assays. Muscle tissue was sampled for hydration content (Muscle Water Content, MWC). Oxygen consumption was not reduced and hemolymph lactate did not increase

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Oxygen uptake and the potentiating effects of increased hemolymph lactate on oxygen transport during exercise in the blue crab,Callinectes sapidus

Cited by 143 publications

References 53 publications

Testing a selective tidal‐stream transport model: Observations of female blue crab (Callinectes sapidus) vertical migration during the spawning season

Testing a selective tidal‐stream transport model: Observations of female blue crab (Callinectes sapidus) vertical migration during the spawning season

Energy metabolism and metabolic depression during exercise inCallinectes sapidus, the Atlantic blue crab: effects of the bacterial pathogenVibrio campbellii

Oxygen consumption remains stable while ammonia excretion is reduced upon short time exposure to high salinity in Macrobrachium acanthurus (Caridae: Palaemonidae), a recent freshwater colonizer

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