Oxygenation of malignant tumors after localized microwave hyperthermia

Vaupel, Peter; Otte, Johannes; Manz, R

doi:10.1007/bf01323754

Cited by 48 publications

(9 citation statements)

References 28 publications

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“…Experimental tumours were grown subcutaneously after injection of ascites cells of DS-sarcoma (0-4 ml; approximately lo4 cellslpl) into the dorsum of the hind foot (for further details see Vaupel et al 1982). Tumours were used in experiments when they reached a volume of between 0.7 and 2.5 ml, 6-10 days following tumour implantation.…”

Section: Materials and Methods 21 Animals And Tumoursmentioning

confidence: 99%

Changes in microregional perfusion, oxygenation, ATP and lactate distribution in subcutaneous rat tumours upon water-filtered IR-A hyperthermia

Kelleher

Engel

Vaupel

1995

International Journal of Hyperthermia

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The effect of hyperthermia on microcirculatory and metabolic parameters in s.c. DS-sarcomas of different sizes on the hind foot dorsum of SD-rats was investigated. Hyperthermia was carried out using a novel water-filtered, infrared-A radiation technique. Heating was performed at a rate of 0.5 degrees C/min until 44 degrees C was achieved in the tumour centre, which was maintained for 60 min. Using a multichannel laser Doppler flowmeter, red blood cell flux could be assessed continuously and at several sites within the tumour tissue simultaneously. Substantial inter-site variations in laser Doppler flux (LDF) were observed during hyperthermia which were independent of tumour size, site of measurement, and temperature at the site of measurement, indicating that single site measurements of tumour LDF are poor predictors of the mean response of a tumour to hyperthermia. When mean LDF was considered, decreases in red blood cell fluxes occurred that were more pronounced the greater the tumour volume. In no case was vascular stasis observed. Hyperthermia did not affect tumour oxygenation substantially. Microregional and global assessment of lactate and ATP concentrations demonstrated increased lactate and decreased ATP levels following hyperthermia. Tumour glucose levels were increased following hyperthermia, possibly due to an enlarged distribution space resulting from development of interstitial oedema. Changes in lactate and ATP levels and the lack of changes in tumour oxygenation suggest a modification of energy metabolism following hyperthermia in the form of increased ATP hydrolysis, intensified glycolysis and impaired oxidative phosphorylation.

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Section: Materials and Methods 21 Animals And Tumoursmentioning

confidence: 99%

Changes in microregional perfusion, oxygenation, ATP and lactate distribution in subcutaneous rat tumours upon water-filtered IR-A hyperthermia

Kelleher

Engel

Vaupel

1995

International Journal of Hyperthermia

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“…There is evidence that mild HT can increase blood perfusion of the heated tissue, preferentially at the beginning of tumor heating (7, 8). It has been reported that this can lead to increased oxygen delivery via an improvement of microcirculation (9). This is especially true in cases when the oxygen demand of the tissue is reduced.…”

Section: Preclinical Evidencementioning

confidence: 99%

Integrating Hyperthermia into Modern Radiation Oncology: What Evidence Is Necessary?

2017

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Hyperthermia (HT) is one of the hot topics that have been discussed over decades. However, it never made its way into primetime. The basic biological rationale of heat to enhance the effect of radiation, chemotherapeutic agents, and immunotherapy is evident. Preclinical work has confirmed this effect. HT may trigger changes in perfusion and oxygenation as well as inhibition of DNA repair mechanisms. Moreover, there is evidence for immune stimulation and the induction of systemic immune responses. Despite the increasing number of solid clinical studies, only few centers have included this adjuvant treatment into their repertoire. Over the years, abundant prospective and randomized clinical data have emerged demonstrating a clear benefit of combined HT and radiotherapy for multiple entities such as superficial breast cancer recurrences, cervix carcinoma, or cancers of the head and neck. Regarding less investigated indications, the existing data are promising and more clinical trials are currently recruiting patients. How do we proceed from here? Preclinical evidence is present. Multiple indications benefit from additional HT in the clinical setting. This article summarizes the present evidence and develops ideas for future research.

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“…On day 5 after implantation tumours that had reached volumes of 0.4-0.6m~ were used for measurements. Further details on growth and pathophysiological characteristics of the sarcomas have been published previously (Vaupel et al 1982). Animal care and experimentation were performed according to the respective federal law and had been approved by the regional animal ethics committee.…”

Section: Animals and Tumoursmentioning

confidence: 99%

Tumour-growth inhibition by induced hyperglycaemia/hyperlactacidaemia and localized hyperthermia

Mueller-Klieser

Walenta

Kelleher

et al. 1996

International Journal of Hyperthermia

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The present study was undertaken to exploit pathophysiological properties of solid tumours for a tumour-specific therapy. Experiments were carried out on DS-sarcomas implanted s.c. in the hind foot dorsum of Sprague Dawley rats. Treatment strategies included tumour acidification, lactate accumulation and disturbance of the microcirculation by induced systemic hyperglycaemia/hyperlact-acidaemia (15-25/10 mmol/L; for 60 min) as well as localized hyperthermia (water-bath; 43 degrees C, 30 min.). A special infusion solution was developed for the systemic treatment containing glucose, lactic acid and organic buffer without inorganic ions. Growth kinetics of tumour volume and animal survival were taken as endpoints in order to quantify therapeutic efficiency. After a single treatment with combined modalities, i.e., with hyperglycaemia/hyperlactacidaemia and hyperthermia, approximately 50% of the tumours showed complete remission in three independent series of experiments; around 40% of the animals survived more than two months. In the untreated control group, all animals died from the disease within 10-15 days after tumour implantation. The overall effect on tumour volume changes of the combined therapy was supra-additive compared to that of treatment with hyperthermia or hyperglycaemia/hyperlactacidaemia alone. However, treated animals either showed a dramatic response to the combination of treatments with complete tumour remission or hardly responded at all, justifying a subdivision into responders and non-responders. Pathophysiological mechanisms responsible for this behaviour have to be elucidated in future studies. Nevertheless, the present study represents an approach to an efficient tumour therapy with a potential application in clinical oncology in the not too distant future.

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Oxygenation of malignant tumors after localized microwave hyperthermia

Cited by 48 publications

References 28 publications

Changes in microregional perfusion, oxygenation, ATP and lactate distribution in subcutaneous rat tumours upon water-filtered IR-A hyperthermia

Changes in microregional perfusion, oxygenation, ATP and lactate distribution in subcutaneous rat tumours upon water-filtered IR-A hyperthermia

Integrating Hyperthermia into Modern Radiation Oncology: What Evidence Is Necessary?

Tumour-growth inhibition by induced hyperglycaemia/hyperlactacidaemia and localized hyperthermia

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