Oxyhaemoglobin blocks non-adrenergic non-cholinergic inhibition in the bovine retractor penis muscle

Bowman, Anne; Gillespie, John; Pollock, David

doi:10.1016/0014-2999(82)90470-8

Cited by 58 publications

(33 citation statements)

References 6 publications

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“…In contrast to oxyhaemoglobin, methaemoglobin has no effect on NO activity (Bowman et al 1982;Radomski, Palmer & Moncada, 1987). In the present experiments, commercially prepared haemoglobin was used as it consists primarily of methaemoglobin.…”

Section: Methaemoglobinmentioning

confidence: 99%

Effect of nitric oxide on circular muscle of the canine small intestine.

Stark

Bauer

Szurszewski

1991

The Journal of Physiology

135

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SUMMARY1. Experiments were designed to determine in circular muscle of the canine jejunum whether exogenous nitric oxide (NO) mimics the non-adrenergic, noncholinergic inhibitory junction potential (NANC IJP), and whether changes in the availability of endogenous NO affects IJP amplitude.2. Mechanical and intracellular electrical activity were recorded simultaneously from circular muscle of the canine jejunum. Electrical field stimulation evoked NANC IJPs and inhibited spontaneous contractions.3. Infusions of NO solutions evoked immediate dose-dependent and transient hyperpolarizations and transiently inhibited spontaneous contractions. NO-evoked hyperpolarizations were unaffected by atropine, propranolol, phentolamine and tetrodotoxin.4. The maximum IJP amplitude and the maximum amplitude of NO-evoked hyperpolarization were similar.5. NG-Mono-methyl-L-arginine (L-NMMA), which inhibits synthesis of NO from Larginine, reduced IJP amplitudes but did not reduce the response to exogenous NO. L-Arginine, but not D-arginine, reversed the effect of L-NMMA on IJP amplitude.6. Oxyhaemoglobin, which binds and inactivates NO, reduced IJP amplitude and abolished the response to exogenous NO. 7. Exogenous NO mimicked the effects of NANC inhibitory input. Reducing the availability of endogenous NO reduced NANC inhibitory input.8. It was concluded that NO mediates NANC neural inhibition and may act as a NANC inhibitory neurotransmitter in the canine jejunum.

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Section: Methaemoglobinmentioning

confidence: 99%

Effect of nitric oxide on circular muscle of the canine small intestine.

Stark

Bauer

Szurszewski

1991

The Journal of Physiology

135

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“…Haemolysate itself caused a reproducible and statistically significant fall in the resting cyclic GMP content of the bovine retractor penis muscle (Table 1). Interestingly, the initial response of the bovine retractor penis muscle to the addition of haemoglobin or haemolysate is a slow rise in tone (Bowman & Gillespie, 1982;Bowman et al, 1982b). …”

Section: Blockade By Haemolysate (Haemoglobin)mentioning

confidence: 99%

Cyclic GMP mediates neurogenic relaxation in the bovine retractor penis muscle

Bowman

Drummond

1984

British J Pharmacology

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1 Field stimulation of the non-adrenergic, non-cholinergic inhibitory nerves to the bovine isolated retractor penis muscle evoked a relaxation that was preceded by a rise in the tissue content of cyclic GMP. There was no change in the content of cyclic AMP. 2 The selective cyclic GMP phosphodiesterase inhibitor, 2-o-propoxyphenyl-8-azapurin-6-one (M&B 22948), elevated the tissue's cyclic GMP content, and potentiated both the relaxation and the rise in cyclic GMP produced by inhibitory nerve stimulation. 3 Sodium nitroprusside and an inhibitory factor extracted from the bovine retractor penis muscle mimicked the effects of inhibitory nerve stimulation in that they each produced relaxation associated with a selective rise in cyclic GMP concentration. 4 Haemoglobin (in the form of erythrocyte haemolysate) and N-methylhydroxylamine, which are known to block guanylate cyclase, blocked the relaxation and the rise in cyclic GMP content produced by inhibitory nerve stimulation, inhibitory factor and sodium nitroprusside. Haemoglobin itself caused a rise in muscle tone and at the same time reduced the cyclic GMP content of the tissue. 5 8-Bromocyclic GMP, a permeant derivative of cyclic GMP, produced a relaxation of the muscle that, as expected, was not blocked by haemoglobin. 6 Vasoactive intestinal polypeptide, prostaglandin E1 and forskolin each produced relaxation associated with a selective rise in cyclic AMP content. Their effects were not blocked by haemoglobin or N-methylhydroxylamine. 7 It is concluded that inhibitory nerve stimulation in the bovine retractor penis muscle produces a relaxation that is mediated by cyclic GMP, although some substances relax the muscle without affecting cyclic GMP levels. The results are also compatible with the view that the extracts of muscle contain the inhibitory neurotransmitter.

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“…relaxation (Bowman et al, 1982;Bowman & McGrath, 1985;Bowman et al, 1986;Gibson & Mirzazadeh, 1989;Gillespie & Sheng, 1990). Furthermore, NANC transmission, like relaxation mediated by EDRF (Rapoport & Murad, 1983;Griffith et al, 1985;Martin et al, 1986), was shown to proceed through elevation of cellular guanosine 3':5'-cyclic monophosphate (cyclic GMP) content and was potentiated by inhibitors of cyclic GMP phosphodiesterase (Bowman & Drummond, 1984).…”

Section: Introductionmentioning

confidence: 99%

Actions and interactions of N^G‐substituted analogues of l‐arginine on NANC neurotransmission in the bovine retractor penis and rat anococcygeus muscles

Martin

Gillespie

Gibson

1993

British J Pharmacology

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1 The effects and interactions of a series of N0-substituted analogues of L-arginine known to inhibit nitric oxide synthase were examined on non-adrenergic, non-cholinergic (NANC) neurotransmission in the bovine retractor penis (BRP) and rat anococcygeus muscles. 2 Treatment of BRP muscle strips with either NG-nitro L-arginine (L-NOARG: 0.1-1OILM) or N0-nitro L-arginine meythl ester (L-NAME; 0.1-100 pM) produced a concentration-dependent blockade of NANC relaxation: blockade was complete at the highest concentration of each. 3 Pretreatment with L-arginine (1-1O mM) had no effect on NANC relaxation by itself, but inhibited, in a concentration-dependent manner, the subsequent ability of both L-NOARG (0.1-300 AM) and L-NAME (0.1-1 mM) to produce blockade. L-Arginine (1-1O mM) reversed established submaximal blockade of NANC relaxation induced by L-NOARG (1 JM) or L-NAME (1 gM), but had little effect on maximal blockade induced by these agents. 4 In contrast to L-NOARG and L-NAME, N0-monomethyl L-arginine (L-NMMA; 1 gAM-1 mM) had no effect by itself on NANC relaxation of the BRP. L-NMMA (0.1-1 mM) did, however, like L-arginine, inhibit, in a concentration-dependent manner, the subsequent ability of both L-NOARG (0.1-1 mM) and L-NAME (0.1-3 mM) to produce blockade, but was more potent. As with L-arginine, L-NMMA (0.1-1 mM) reversed established submaximal blockade of NANC relaxation induced by L-NOARG (1 gM) or L-NAME (1 JM), but had little effect on maximal blockade induced by these agents.5 In contrast to the effects on BRP, treatment of rat anococcygeus muscle with either L-NOARG (0.1-1OJM) or L-NMMA (1-100JM) produced concentration-dependent inhibition of NANC relaxation: the maximal inhibition induced by L-NOARG and L-NMMA was 100% and 40.1 ± 5.9% (n = 8), respectively. L-Arginine (1-10 mM) reversed established submaximal inhibition of NANC relaxation induced by L-NOARG (1 JM), had little effect on maximal blockade by this agent, and reversed maximal blockade induced by L-NMMA (100IJM). 6 In the presence of partial blockade of NANC relaxation on rat anococcygeus by a maximal concentration of L-NMMA (100IJM), subsequent blockade by L-NOARG (0.1-100IM) was inhibited. L-NMMA (100 JM) produced a partial reversal of established submaximal blockade of NANC relaxation induced by L-NOARG (1 JAM), but had little effect on maximal blockade induced by this agent. 7 These findings suggest a complex series of interactions between L-arginine and certain of its NG-substituted analogues that are commonly used to inhibit nitric oxide synthase. The most striking new finding is that L-NMMA does not block NANC relaxation in the BRP, but acts with greater potency than the endogenous substrate, L-arginine, to inhibit the blockade induced by L-NOARG or L-NAME. Even on rat anococcygeus where L-NMMA acts as a partial blocker of NANC relaxation, further blockade by L-NOARG is inhibited.

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Oxyhaemoglobin blocks non-adrenergic non-cholinergic inhibition in the bovine retractor penis muscle

Cited by 58 publications

References 6 publications

Effect of nitric oxide on circular muscle of the canine small intestine.

Effect of nitric oxide on circular muscle of the canine small intestine.

Cyclic GMP mediates neurogenic relaxation in the bovine retractor penis muscle

Actions and interactions of N^G‐substituted analogues of l‐arginine on NANC neurotransmission in the bovine retractor penis and rat anococcygeus muscles

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Oxyhaemoglobin blocks non-adrenergic non-cholinergic inhibition in the bovine retractor penis muscle

Cited by 58 publications

References 6 publications

Effect of nitric oxide on circular muscle of the canine small intestine.

Effect of nitric oxide on circular muscle of the canine small intestine.

Cyclic GMP mediates neurogenic relaxation in the bovine retractor penis muscle

Actions and interactions of NG‐substituted analogues of l‐arginine on NANC neurotransmission in the bovine retractor penis and rat anococcygeus muscles

Contact Info

Product

Resources

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Actions and interactions of N^G‐substituted analogues of l‐arginine on NANC neurotransmission in the bovine retractor penis and rat anococcygeus muscles