Oxylipin and mitochondrion probes to track yeast sexual cells

Ncango, Desmond M.; Swart, Chantel W.; Goldblatt, Monique E.; Pohl, Carolina H.; Wyk, Pieter van; Botes, P.J.; Kock, J.L.F.

doi:10.1139/w08-035

Cited by 6 publications

(3 citation statements)

References 20 publications

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“…A separate study found EMA treatment at concentrations of 10 to 25 µg mL -1 , followed by a 2 minute halogen light exposure, yielded successful qPCR of DNA from viable E. coli cells (Inoue et al, 2008). Cleland et al (2008) In order to detect cells with mitochondrial activity, fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH) oxylipins, which are mitochondriallyproduced, and rhodamine 123 (Rh123), which accumulate in functional mitochondria, were successfully added to a culture of yeast cells to detect reproducing cells (Ncango et al, 2008).…”

Section: Other New Methodsmentioning

confidence: 99%

Molecular Methods in Biological Systems

Reyes

Oerther

Angenent

2010

Water Environment Research

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In 2009, molecular methods continue to be widely applied in different areas of environmental engineering research. Fingerprinting techniques such as denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T‐RFLP) and automated ribosomal intergenic spacer analysis (ARISA) continue to be popular tools to track differences in microbial community composition over time and space. Fluorescent in situ hybridization (FISH) of whole cells was also commonly employed to observe microbes of interest in ecological systems. Real time quantitative PCR methods appear to have become much more mainstream and were used to conduct comparative analyses of communities in different systems and over time. Several data analysis methods and software was proposed to analyze large and complex genomic data. In this review we chose to focus on new or significantly revised methods, novel applications of existing methods, and a few high quality reviews published in 2009, since the body of literature using molecular methods in environmental engineering has become very large.

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Section: Other New Methodsmentioning

confidence: 99%

Molecular Methods in Biological Systems

Reyes

Oerther

Angenent

2010

Water Environment Research

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“…Liu and colleagues (2008) In order to detect cells with mitochondrial activity, fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH) oxylipins, which are mitochondriallyproduced, and rhodamine 123 (Rh123), which accumulate in functional mitochondria, were successfully added to a culture of yeast cells to detect reproducing cells (Ncango et al, 2008).…”

Section: Other New Methodsmentioning

confidence: 99%

Molecular Methods in Biological Systems

McMahon¹,

Gu²,

Nerenberg³

et al. 2009

Water Environment Research

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In 2008, molecular methods continue to be mainstream in environmental engineering research. Fingerprinting techniques such as denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T‐RFLP) analysis continue to be popular tools to track differences in microbial community composition over time and space. Fluorescent in situ hybridization (FISH) of whole cells was also commonly employed. Real time quantitative PCR methods appear to have become much more mainstream and were used to conduct comparative analyses of communities in different systems and over time. In this review we chose to focus on new or significantly revised methods, novel applications of existing methods, and a few high quality reviews published in 2008, since the body of literature using molecular methods in environmental engineering has become very large.

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“…Tel: +27 51 401 2249. Fax: +27 401 9376. in most ascomycetous yeasts except in Zygosaccharomyces (Kock et al, 2007;Ncango et al, 2008;Swart et al, 2008Swart et al, , 2010a.…”

Section: Introductionmentioning

confidence: 99%

The influence of mitochondrial inhibitors on the life cycle of Phytophthora

Swart¹,

Wyk²,

Pohl³

et al. 2011

Afr. J. Microbiol. Res.

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The anti-mitochondrial antifungal hypothesis implies a link between mitochondrial activity, fungal fruiting structures and susceptibility towards mitochondrial inhibitors. Here it is shown that the oomycete, Phytophthora nicotianae fits the hypothesis. Fruiting structures (zoosporangia) of this oomycete showed increased beta ()-oxidation when probing levels of 3-hydroxy fatty acids (3-OH oxylipins) with specific polyclonal antibodies. In addition, increased mitochondrial activity was also observed in the zoosporangia when the mitochondrial transmembrane potential (m) probe, Rhodamine 123 was added to the culture. This indicates increased mitochondrial activity in the zoosporangia when compared to the hyphae. When the anti-mitochondrial drug acetylsalicylic acid (ASA) was added to cultures of this oomycete, the zoosporangia were, as expected, most susceptible and were drastically inhibited in the presence of 1 mM of this compound. Similar ASA inhibition results were recorded for P. citrophthora. It is concluded that anti-mitochondrial compounds may find application in combating these devastating plant pathogens and that urgent further research is needed in this direction.

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Oxylipin and mitochondrion probes to track yeast sexual cells

Cited by 6 publications

References 20 publications

Molecular Methods in Biological Systems

Molecular Methods in Biological Systems

Molecular Methods in Biological Systems

The influence of mitochondrial inhibitors on the life cycle of Phytophthora

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