Oxymatrine diminishes the side population and inhibits the expression of β-catenin in MCF-7 breast cancer cells

Zhang, Ying; Bing-kui, Piao; Zhang, Yong; Bai, Hua; Hou, Wei; Xu, Wenxiang; Qi, Xin; Zhu, Xiaoyun; Pei, Yingxia; Lin, Hongsheng

doi:10.1007/s12032-010-9721-y

Cited by 71 publications

(65 citation statements)

References 23 publications

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“…25) Moreover, oxymatrine caused a dose-dependent reduction in the number of SP cells via Wnt/β-catenin signaling pathway. Thus, oxymatrine has the potential to suppress breast cancer progression by inhibiting cancer stem-like cells in addition to its inhibitory effects on cancer cell proliferation and single cell proliferation capacity.…”

Section: Discussionmentioning

confidence: 99%

Oxymatrine Promotes S-Phase Arrest and Inhibits Cell Proliferation of Human Breast Cancer Cells <i>in Vitro</i> through Mitochondria-Mediated Apoptosis

Cai

et al. 2017

Biological & Pharmaceutical Bulletin

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Breast cancer is one of the most lethal malignancies in the world. Oxymatrine is the major effective and toxic alkaloid component which is derived from the root of Sophora flavescens AIT, a traditional Chinese medicine which is widely distributed in Asia and the Pacific Islands. In the current research study, we investigated the effects and mechanisms of action of oxymatrine on breast cancer cells. We demonstrated that the viability and single cell proliferation capability of MCF-7 and MDA-MB-231, two breast cancer cell lines which are widely used in in vitro study, were significantly suppressed in a time-and concentration-dependent manner. Furthermore, the cell cycle of breast cancer cells treated with oxymatrine was arrested at the Sphase of the cell cycle. Oxymatrine also triggered apoptosis in breast cancer cells by modulating apoptosisrelated proteins, such as cleaved Caspase-3, cleaved Caspase-9 and poly(ADP-ribose) polymerase (PARP). The remarkable reduction in the ratio of Bcl-2/Bax was also observed in oxymatrine treated breast cancer cells.In conclusion, our research demonstrated that oxymatrine plays a critical role in suppressing carcinogenesis of breast cancer cells through cell cycle arrest and induction of mitochondria-mediated apoptosis, which suggests a promising application of this drug in breast cancer therapy.Key words oxymatrine; apoptosis; breast cancer; cell cycle Breast cancer is the most highly prevalent and diagnosed carcinoma in women worldwide and is the leading cause of cancer deaths in women, with approximately 500000 newly diagnosed cases reported in 2012.1) Currently, the main treatment options for breast cancer cases include surgery, radiotherapy, chemotherapy, hormone and immunological therapy. Although effectiveness could be observed in breast cancer patients receiving a variety of therapies, the high toxicity of these treatments to normal tissues is practically inevitable. Furthermore, breast cancer cells often propagate distant metastases and multi-drug resistance, which represents a main cause for treatment failure in cancer patients.2-4) Thus, the search for novel effective therapies and targeted drugs are an urgent priority with the aim of improving the efficacy of breast cancer treatment.Traditional Chinese medicine has been shown to be a fertile source of novel drug discovery. Many active components which are extracted from traditional Chinese herbs exhibit effects in many human diseases.5-7) For example, oxymatrine is the main effective and toxic alkaloid component derived from the root of Sophora flavescens AIT, which is widely distributed in Asia and the Pacific Islands.8-10) It was previously reported that oxymatrine displays specific pharmacological effects in targeting hepatitis B infections and liver fibrosis. [11][12][13] Furthermore, it has been demonstrated that oxymatrine exerts anti-tumor properties in several cancers via different signaling pathways, such as suppression of proliferation and promotion of apoptosis. [14][15][16][17] Although previous studies s...

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Section: Discussionmentioning

confidence: 99%

Oxymatrine Promotes S-Phase Arrest and Inhibits Cell Proliferation of Human Breast Cancer Cells <i>in Vitro</i> through Mitochondria-Mediated Apoptosis

Cai

et al. 2017

Biological & Pharmaceutical Bulletin

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“…66 The Wnt/β-Catenin signal pathway is also activated in various tumors and involved in the regulation of the self-renewal of CSCs. In breast cancer, the natural compound sulforaphane inhibiting the self-renewal of ALDH positive cells, 38 and oxymatrine suppressing the the self-renewal of SP cells, 67 are all associated with downregulation of the Wnt/β-Catenin signaling pathway.…”

Section: Signaling Pathway Inhibitorsmentioning

confidence: 99%

Therapeutic strategies targeting cancer stem cells

Ning

Shu

et al. 2013

Cancer Biology & Therapy

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“…The treatment of breast cancer cells (MCF-7) with oxymatrine was associated with an inhibition of cell proliferation and a decrease in the number of stem cell-like side population (SP) cells. The anti-cancer effect of oxymatrine on MCF-7 cells may be due to the inhibition of the Wnt/β-catenin signaling pathway [23] . Oxymatrine can also induce apoptosis in human pancreatic cancer PANC-1 cells by a mechanism similar to matrine.…”

Section: Introductionmentioning

confidence: 99%

Anti-tumor activities of active ingredients in Compound Kushen Injection

Wang¹,

et al. 2015

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Kushen (Radix Sophorae Flavescentis) has a long history of use for the treatment of tumors, inflammation and other diseases in traditional Chinese medicine. Compound Kushen Injection (CKI) is a mixture of natural compounds extracted from Kushen and Baituling (Rhizoma Smilacis Glabrae). The main principles of CKI are matrine (MT) and oxymatrine (OMT) that exhibit a variety of pharmacological activities, including anti-inflammatory, anti-allergic, anti-viral, anti-fibrotic and cardiovascular protective effects. Recent evidence shows that these compounds also produce anti-cancer actions, such as inhibiting cancer cell proliferation, inducing cell cycle arrest, accelerating apoptosis, restraining angiogenesis, inducing cell differentiation, inhibiting cancer metastasis and invasion, reversing multidrug resistance, and preventing or reducing chemotherapy-and/or radiotherapy-induced toxicity when combined with chemotherapeutic drugs. In this review, we summarize recent progress in studying the anti-cancer activities of MT, OMT and CKI and their potential molecular targets, which provide clues and references for further study.

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Oxymatrine diminishes the side population and inhibits the expression of β-catenin in MCF-7 breast cancer cells

Cited by 71 publications

References 23 publications

Oxymatrine Promotes S-Phase Arrest and Inhibits Cell Proliferation of Human Breast Cancer Cells <i>in Vitro</i> through Mitochondria-Mediated Apoptosis

Oxymatrine Promotes S-Phase Arrest and Inhibits Cell Proliferation of Human Breast Cancer Cells <i>in Vitro</i> through Mitochondria-Mediated Apoptosis

Therapeutic strategies targeting cancer stem cells

Anti-tumor activities of active ingredients in Compound Kushen Injection

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