Oxymatrine inhibited cell proliferation by inducing apoptosis in human lung cancer A549 cells

Abstract

Abstract.To investigate the inhibition effect of oxymatrine induces human lung cancer A549 cells apoptosis. The A549 cells were cultured for 24 h, than the various concentration of oxymatrine (2 mmol/L, 4 mmol/L, 8 mmol/L, 15 mmol/L) were added into different experimental group cells, and 5-fluorouracil were added into the positive control group cells for 12 h, 24 h, 36 h, 48 h respectively. The A549 cells inhibition rate, apoptosis, and the expression of Bcl-2 and Bax were examined by MTT method, Annexin /PI double staining method, real-time quantitative PCR and western blot, respectively. At same time, the morphological changes of A549 cells were observed with an inverted microscope. In the range of 2 mmol/L~15 mmol/L, oxymatrine had obvious inhibition effects on the proliferation of A549 cells. Compared with the negative control group, it has significantly different (P<0.01). There was remarkably the time-and dose-dependent correlation. After A549 cells were treated with 8 mmol/L oxymatrine for 24 h, the morphological change of cell apoptosis was observed and the extent of apoptosis was quantified by flow cytometry. Furthermore, the expression of Bcl-2 was reduced and the expression of Bax was increased remarkably (P<0.05). Oxymatrine has significant inhibition effects on the cells proliferation and the effects showed time-dependent and dose-dependent. Oxymatrine can induce apoptosis of the A549 cells by regulating the expression of Bcl-2 and Bax.

Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells

“…OMT has been extensively investigated for antitumor effects against various cancers [15], including lung [12], gastric [13], pancreatic [11], and breast [14]. However, to the best of our knowledge, the antitumor effects of OMT on GBM have yet to be fully investigated.…”

“…It is widely understood that caspase-3 is an essential terminal caspase that executes apoptosis and eventually leads to DNA fragmentation in the mitochondrial pathway [19, 21]. Therefore, induction of apoptosis may represent a potential approach for antitumor therapy [22], and there is an increasing body of evidence that OMT may induce apoptosis in a number of cancer cell lines [11, 12, 15, 18, 23–26]. The data presented herein adds to this body of evidence by demonstrating that OMT induces apoptosis, decreases expression of Bcl-2, and concurrently increases expression of Bax and caspase-3.…”

Oxymatrine Promotes S-Phase Arrest and Inhibits Cell Proliferation of Human Breast Cancer Cells <i>in Vitro</i> through Mitochondria-Mediated Apoptosis

“…It has been demonstrated that oxymatrine promotes anti-tumor properties in the setting of many carcinomas, including colon carcinoma, hepatocellular carcinoma and lung cancer. [19][20][21][22] However, the signaling pathways which mediate its anti-tumor properties are divergent. In colorectal cancer, epithelial-mesenchymal transition (EMT), which is involved in the initiation of metastasis for malignant cancer progression was inhibited by oxymatrine through regulation of the nuclear factor-kappaB (NF-κB) signaling pathway.…”

Understanding the Effectiveness of Natural Compound Mixtures in Cancer through Their Molecular Mode of Action

“…It is also known that a mixture of oxymatrine and micellar nanoparticles is an effective proliferation inhibitor of SMM7721 cells [52]. Oxymatrine treatment significantly induces apoptosis by increasing Bax protein expression and reducing Bcl-2 in human lung cancer A549 cells [53]. Proteomic analysis has shown that oxymatrine induces apoptosis in HeLa cells by inhibiting inosine monophosphate dehydrogenase type II (IMPDH2), mitochondrial related apoptotic protein [54].…”