Oxytetracycline induces anxiety-like behavior in adult zebrafish

Gusso, Darlan; Altenhofen, Stefani; Fritsch, Pâmella Moreira; Rübensam, Gabriel; Bonan, Carla Denise

doi:10.1016/j.taap.2021.115616

Cited by 25 publications

(10 citation statements)

References 56 publications

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“…Adult zebrafish has a well-characterized behavioral repertoire ( Kalueff et al, 2013 ) and consistent protocols to evaluate them under experimental conditions ( Nery et al, 2014 , 2017 ; Altenhofen et al, 2017a ; Bridi et al, 2017 ; Nabinger et al, 2018 ; Nabinger et al, 2020 ; Wiprich et al, 2020 ; Gusso et al, 2021 ). This is, however, the first study assessing the acute toxic effects of diuron on the adult zebrafish behavior.…”

Section: Discussionmentioning

confidence: 99%

“…On each smaller side wall of the experimental tank was a glass tank, identically sized (10 cm long × 15 cm high × 10 cm wide): one without fish and one with six adult zebrafish, the latter designated as the “stimulus tank.” To quantify social interaction and innate preference for conspecifics, the experimental tank was virtually divided into three parts: a “stimulus zone” closer to the “stimulus tank” and a “non-stimulus zone” closer to the empty tank, separated by a third central one of equal size. Individual preferences between the stimulus tank zone and the non-stimulus tank zone were automatedly analyzed using EthoVision XT ® tracking software (version 11.5, Noldus) ( Gusso et al, 2021 ). Data are presented as mean time (s) spent by individuals from each group in the stimulus zone.…”

Section: Methodsmentioning

confidence: 99%

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Atrazine and Diuron Effects on Survival, Embryo Development, and Behavior in Larvae and Adult Zebrafish

et al. 2022

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Atrazine and Diuron are widely used herbicides. The use of pesticides contaminates the aquatic environment, threatening biodiversity and non-target organisms such as fish. In this study, we investigated the effects of acute exposure for 96 h hours to atrazine and diuron commercial formulations in zebrafish (Danio rerio, wild-type AB) embryos and larvae and adult stages. We observed a significant concentration-dependent survival decrease and hatching delays in animals exposed to both herbicides and in the frequency of malformations compared to the control groups. Morphological defects included cardiac edema, tail reduction, and head malformation. At 7 days post-fertilization (dpf), atrazine exposure resulted in a reduction in the head length at 2, 2.5, and 5 mg/L and increased the ocular distance at 1, 2, 2.5, and 5 mg/L atrazine when compared to controls. At the same age, diuron increased the ocular distance in animals exposed to diuron (1.0 and 1.5 mg/L) and no effects were observed on the head length. We also evaluated a behavioral repertoire in larvae at 7 dpf, and there were no significant differences in distance traveled, mean speed, time in movement, and thigmotaxis for atrazine and diuron when animals were individually placed in a new environment. The cognitive ability of the larvae was tested at 7 dpf for avoidance and optomotor responses, and neither atrazine nor diuron had significant impacts when treated groups were compared to their corresponding controls. Adults’ behavior was evaluated 7 and 8 days after the end of the acute herbicide exposure. Exploration of a new environment and associated anxiety-like parameters, social interaction, and aggressiveness were not altered. Our results highlight the need for further studies on the sublethal effects of both herbicides and the consideration of the effects of commercial formulas vs. isolated active ingredients. It also emphasizes the need to take sublethal effects into consideration when establishing the environmental limits of residues.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Atrazine and Diuron Effects on Survival, Embryo Development, and Behavior in Larvae and Adult Zebrafish

et al. 2022

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“…From the other side, prolonged exposure to tetracycline alters the levels of triiodothyronine and thyroid-stimulating hormone which might be the sign of the hypothalamus–pituitary–thyroid axis involvement. In addition, a decrease in cortisol levels is observed after tetracycline exposure, which impacts anxiety-related behaviors [ 65 ].…”

Section: The Adverse Effect Of Tetracycline Antibiotics On the Aquati...mentioning

confidence: 99%

The Impact of Tetracycline Pollution on the Aquatic Environment and Removal Strategies

et al. 2023

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Antibacterial drugs are among the most commonly used medications in the world. Tetracycline is a widely used antibiotic for human and animal therapy due to its broad-spectrum activity, high effectiveness, and reasonable cost. The indications for treatment with tetracycline include pneumonia, bone and joint infections, infectious disorders of the skin, sexually transmitted and gastrointestinal infections. However, tetracycline has become a serious threat to the environment because of its overuse by humans and veterinarians and weak ability to degrade. Tetracycline is capable of accumulating along the food chain, causing toxicity to the microbial community, encouraging the development and spread of antibiotic resistance, creating threats to drinking and irrigation water, and disrupting microbial flora in the human intestine. It is essential to address the negative impact of tetracycline on the environment, as it causes ecological imbalance. Ineffective wastewater systems are among the main reasons for the increased antibiotic concentrations in aquatic sources. It is possible to degrade tetracycline by breaking it down into small molecules with less harmful or nonhazardous effects. A range of methods for physical, chemical, and biological degradation exists. The review will discuss the negative effects of tetracycline consumption on the aquatic environment and describe available removal methods.

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“…[55,56] Triclosan, a widely used synthetic antimicrobial agent with poly-target (antibiotic and antifungal) action, also inhibits dopamine and increases acetylcholine neurotransmission, promotes neuronal apoptosis, and reduces synaptic density and axonal length in zebrafish [35]. Furthermore, triclosan downregulates the expression of brain genes that are important during neurodevelopment, including glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) that control myelination and axonal maintenance [57]. In contrast, mir-137, a short non-coding RNA associated with the mitogen-activated protein kinase (MAPK) pathway, is upregulated in zebrafish by triclosan, eventually impairing their auditory and visual sensitivity [36].…”

Section: Essential Oils Extract From Thymus Vulgarismentioning

confidence: 99%

Understanding CNS Effects of Antimicrobial Drugs Using Zebrafish Models

Kotova

Galstyan

Колесникова

et al. 2023

Veterinary Sciences

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Antimicrobial drugs represent a diverse group of widely utilized antibiotic, antifungal, antiparasitic and antiviral agents. Their growing use and clinical importance necessitate our improved understanding of physiological effects of antimicrobial drugs, including their potential effects on the central nervous system (CNS), at molecular, cellular, and behavioral levels. In addition, antimicrobial drugs can alter the composition of gut microbiota, and hence affect the gut–microbiota–brain axis, further modulating brain and behavioral processes. Complementing rodent studies, the zebrafish (Danio rerio) emerges as a powerful model system for screening various antimicrobial drugs, including probing their putative CNS effects. Here, we critically discuss recent evidence on the effects of antimicrobial drugs on brain and behavior in zebrafish, and outline future related lines of research using this aquatic model organism.

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Oxytetracycline induces anxiety-like behavior in adult zebrafish

Cited by 25 publications

References 56 publications

Atrazine and Diuron Effects on Survival, Embryo Development, and Behavior in Larvae and Adult Zebrafish

Atrazine and Diuron Effects on Survival, Embryo Development, and Behavior in Larvae and Adult Zebrafish

The Impact of Tetracycline Pollution on the Aquatic Environment and Removal Strategies

Understanding CNS Effects of Antimicrobial Drugs Using Zebrafish Models

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