Oxytocin ameliorates cisplatin-induced nephrotoxicity in Wistar rats

Elberry, Ahmed A.; Refaie, Shereen M.; Kamel, Mohamed W.; Ali, Tarek M.; Darwish, H.A.; Ashour, Osama M.

doi:10.5144/0256-4947.2013.57

Cited by 13 publications

(12 citation statements)

References 40 publications

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“…Much evidence shows that oxidative stress plays a vital role in the occurrence of neurodegenerative diseases and CNS disorders [26]. Previous studies have claimed that oxytocin has antioxidant properties and protects different tissues from oxidative damage [27,28]. In contrast to these studies, oxytocin did not show antioxidant properties in this study; instead, it aggravates the levels of oxidative stress after H 2 O 2 -induced oxidative damage in C6 cells.…”

Section: Discussioncontrasting

confidence: 91%

Oxytocin Promotes C6 Glial Cell Death and Aggravates Hydrogen Peroxide-induced Oxidative Stress

Taşkıran

Ergül

2020

INNOSC Theranostics and Pharmacological Sciences

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Background. Recent studies have shown that oxytocin plays a vital role in neurons and glial cells. However, its effect on hydrogen peroxide (H2O2)-induced oxidative stress as well as cyclooxygenase-1 (COX-1) and COX-2 in glial cells are still unclear. Objective. This study aims to examine the effect of oxytocin on glial cell viability, oxidative stress, COX-1, and COX-2 in C6 glial cells after exposure to H2O2. Methods. In this study, C6 glioma cell line was used to study the effect of oxytocin on the glial cell in four cell groups. The control group was untreated. Cells in the H2O2 group were treated with 0.5 mM H2O2 for 24 h. Cells in the oxytocin group were treated with various concentrations (0.25, 0.5, 1, and 2 μg/mL) of oxytocin for 24 h. Cells in the oxytocin+H2O2 group were pre-treated with various concentrations (0.25, 0.5, 1, and 2 μg/mL) of oxytocin for 1 h before 24-h exposure to 0.5 mM H2O2. Cell viability was evaluated using XTT assay. Total antioxidant status and total oxidant status (TOS), COX-1, and COX-2 levels in the cells were measured by commercial kits. Results. Oxytocin with various concentrations significantly decreased the viability of C6 cells after H2O2-induced oxidative stress (P < 0.01). It also significantly increased the levels of TOS, COX-1, and COX-2 in C6 cells after H2O2-induced oxidative stress (P < 0.001). Conclusion. Oxytocin increases glial cell death after H2O2-induced oxidative damage in C6 cells, along with upregulation of COX-1 and COX-2 levels.

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Section: Discussioncontrasting

confidence: 91%

Oxytocin Promotes C6 Glial Cell Death and Aggravates Hydrogen Peroxide-induced Oxidative Stress

Taşkıran

Ergül

2020

INNOSC Theranostics and Pharmacological Sciences

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“…We could not identify any article that addressed the same combination therapy either before or after cisplatin. However, sildenafil was shown to improve kidney function and attenuate experimental cisplatin-induced nephrotoxicity [ 11–28 , 40 , 41 ]. Also gemfibrozil was shown to attenuate cisplatin nephrotoxicity either alone or in combination with silymarin [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of combination sildenafil and gemfibrozil on cisplatin-induced nephrotoxicity; role of heme oxygenase-1

et al. 2018

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Background/aim: Cisplatin-induced nephrotoxicity in large proportion of patients. The aim of this work is to clarify the effect of combination of sildenafil and gemfibrozil on cisplatin-induced nephrotoxicity either before or after cisplatin treatment and determination of nephrotoxicity predictors among the measured tissue markers.Methods: Thirty two adult male albino rats were divided into four equal groups (G) GI control, GII received cisplatin, GIII received sildenafil and gemfibrozil before cisplatin, GIV received sildenafil and gemfibrozil after cisplatin. Creatinine and urea were measured and animals were sacrificed and kidney was taken for histopathology. The following tissue markers were measured, heme oxygenase-1 (HO-1) activity, reduced glutathione, quantitative (real-time polymerase chain reaction) RT-PCR for gene expression of tumor necrosis factor alpha (TNF-α) and endothelial nitric oxide synthase (ENOS) level.Results: GII developed AKI demonstrated by significantly high urea and creatinine and severe diffuse (80–90%) tubular necrosis. TNF-α was highly and significantly elevated while the rest of tissue markers were significantly reduced in GI1 compared to other groups. GIV showed better results compared to GIII. There was a significant positive correlation between creatinine and TNF-α when combining GI and GII while there were significant negative correlation between creatinine and other tissue markers in same groups. Linear regression analysis demonstrated that HO-1 was the independent predictor of AKI demonstrated by elevated creatinine among GI and GII.Conclusions: Combination of sildenafil and gemfibrozil can be used in treatment of cisplatin-induced nephrotoxicity. HO-1 is a promising target for prevention and/or treatment of cisplatin-induced nephrotoxicity.

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“…To the Editor: We read with great interest the recently published nice article in the esteemed “Ann Saudi Med”, by Elberry et al entitled “Oxytocin ameliorates cisplatin-induced nephrotoxicity in Wistar rats”. 1 In an experimental study on 48 male Wistar albino rats, Elberry et al aimed to study the ameliorative effects of oxytocin against cisplatin renal toxicity. 1 They assessed the renal injury of cisplatin by performing histopathological study and by observing an increase in serum LDH activity as well as urea and creatinine levels.…”

mentioning

confidence: 99%

“… 1 In an experimental study on 48 male Wistar albino rats, Elberry et al aimed to study the ameliorative effects of oxytocin against cisplatin renal toxicity. 1 They assessed the renal injury of cisplatin by performing histopathological study and by observing an increase in serum LDH activity as well as urea and creatinine levels. Furthermore, to asses renal injury, they also measured renal tissue activities of catalase, superoxide dismutase, glutathione peroxidase, and glutathione S-transferase as well as glutathione level.…”

mentioning

confidence: 99%

“…They found that oxytocin protected rats from cisplatin-induced nephrotoxicity and attributedthis to the antioxidant activity of oxytocin. 1 In this letter, I would like to point out a few points about cisplatin nephrotoxicity. In a preclinical study, we observed that estrogen attenuates the defending property of erythropoietin against cisplatin-induced renal toxicity in ovariectomized Wistar rats.…”

mentioning

confidence: 99%

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