Oxytocin differentially effects 3β-hydroxysteroid dehydrogenase and 5α-reductase activities in prostate cancer cell lines

Assinder, Stephen J.; Davies, K. E.; Surija, Jonathan; Liu-Fu, Frank

doi:10.1016/j.peptides.2015.07.015

Cited by 4 publications

(4 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, OT is involved in regulating the conversion from testosterone to dihydrotestosterone by differential regulation of 5AR I/II [ 25 ]. It is also known that oxytocin stimulates steroidogenesis in several organs by modulating activity of 3-hydroxysteroid dehydrogenases and steroid 5-reductases in both the androgen-dependent LNCaP and androgen-independent PC-3 human prostate cancer cell lines [ 26 ]. Thus, OT contributes considerably to the prostatic disease development.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies, have proved that OT stimulates PCa growth and that a Gi-dependent mechanism is involved in the OTR-mediated migration of prostate cancer cells. Some also argued that OT influences 3-hydroxysteroid dehydrogenase and 5-reductase activities in PCa cell lines and this effect may represent an important step of oxytocinergic regulation of steroidogenesis in PCa [ 17 ]. Besides, many GPCR tend to interact with APPL1, a protein that was originally treated as an Akt2-binding protein, whose unique structures include an adaptor protein with a pleckstrin homology (PH) domain, a phosphotyrosine binding (PTB) domain and a leucine zipper motif.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The function of oxytocin: a potential biomarker for prostate cancer diagnosis and promoter of prostate cancer

Chen

et al. 2017

Oncotarget

View full text Add to dashboard Cite

PurposeTo measure the level of oxytocin in serum and prostate cancer (PCa) tissue and study its effect on the proliferation of PCa cells.ResultsOxytocin level in serum was significantly increased in PCa patients compared with the no-carcinoma individuals. Additionally, the levels of oxytocin and its receptor were also elevated in the PCa tissue. However, no significant difference existed among the PCa of various Gleason grades. Western blot analysis confirmed the previous results and revealed an increased expression level of APPL1.Materials and methodsThe level of oxytocin in serum was measured by ELISA analysis. The expression of oxytocin and its receptor in prostate was analyzed by immunohistochemistry. The proliferation and apoptosis of PCa cells were assessed by the Cell Counting Kit 8 (CCK8) assay, cell cycle analysis and caspase3 activity analysis, respectively. Western blot analysis was used for the detection of PCNA, Caspase3 and APPL1 protein levels.ConclusionsSerum and prostatic oxytocin levels are increased in the PCa subjects. Serum oxytocin level may be a biomarker for PCa in the future. Oxytocin increases PCa growth and APPL1 expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The function of oxytocin: a potential biomarker for prostate cancer diagnosis and promoter of prostate cancer

Chen

et al. 2017

Oncotarget

View full text Add to dashboard Cite

show abstract

“…Particularly in pregnant women, OXT contributes signi cantly to the milk injection re ex and uterine contractile activity for smooth childbirth [10]. In addition, OXT regulates the activity of steroidogenic enzymes and steroidogenesis in several organs, including the gonads (testes and ovaries) [11]. Although the physiological functions and signaling pathways of OXT in the brain, uterus, and reproductive organs have been well established, research on the placenta is limited.…”

Section: Introductionmentioning

confidence: 99%

Oxytocin modulates steroidogenesis-associated genes and estradiol levels in the placenta

Kim

Jeong

et al. 2023

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

BackgroundOxytocin (OXT) plays a signi cant role during pregnancy, especially toward the end of pregnancy. Some studies have reported that OXT is involved in the stimulation of steroidogenesis in several organs. However, the effects of OXT on placental steroidogenesis have not yet been established. MethodsIn this study, we investigated the regulation of steroid hormones and steroidogenic enzymes by OXTassociated signaling in vitro and in vivo by western blotting analysis, ELISA and immunocytochemical analysis. ResultsOXT increased the gene expression of steroidogenic enzymes, which convert pregnenolone to progesterone and dehydroepiandrosterone (DHEA) in vitro. In OXT-administered pregnant rats, pregnenolone and DHEA levels were signi cantly enhanced in the plasma by 2.73-fold and 1.53-fold, respectively and the expression of the enzymes synthesizing DHEA, testosterone, and estradiol (E2) was increased in placental tissues. Furthermore, OXT was found to affect placental cell differentiation, which is closely related to steroid hormone synthesis. After treatment of the pregnant rats with atosiban, an antagonist of the OXT receptor, the concentration of E2 in the plasma and the expression of E2synthesizing enzyme were reduced 1.71-fold and 2.38-3.16-fold, respectively. This regulation may be due to OXT-mediated differentiation, because OXT increases the expression of corticotropin-releasing hormone, which is a biomarker of placental cell differentiation. ConclusionsOur ndings suggest that OXT regulates the differentiation of placental cells, expression of steroidogenic enzymes in the placenta, and production of critical steroid hormones during pregnancy, and it contributes to the maintenance of pregnancy.

show abstract

“…Oxytocin has numerous peripheral actions such as lactation, smooth muscle contraction, wound healing, natriuresis, sexual behaviour and mostly known as social behaviour hormone which increases trust and reduces fear, monogamous pair and maternal bonding (Veening et al, 2015;Jurek and Neumann, 2018). Oxytocin stimulates steroidogenesis in several organs by modulating activity of 3hydroxysteroid dehydrogenases and steroid 5αreductases (5AR) in both the androgendependent LNCaP and androgen-independent PC-3 human prostate cancer cell lines (Assinder et al, 2015). It is also known to modulate sperm production, contractility of the male tract to regulate sperm transport and maturation (Thackare et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Alterations in the histology of the prostate of Sprague-Dawley rats treated with oxytocin

Odetola¹,

Idaguko²

2022

Anat. J. Afr.

View full text Add to dashboard Cite

Background: The role of oxytocin in female reproductive system has been well studied. Very little is known about the long-term administration of oxytocin in prostatic tissues. This study aimed to assess the histological effects of prolonged administration of exogenous oxytocin on prostate in the male Sprague-Dawley rats. Methods: Twenty adult male Sprague-Dawley rats weighing between 180-250g were randomly distributed into four groups A, B, C and D of five rats each. Group A served as the control while groups B to D were the treated groups. Oxytocin was administered intramuscularly two days per week at the doses of 1, 2 and 3 IU/kg/b.w. to groups B, C and D respectively while 0.5ml of physiologic saline was administered to the control. The treatment was carried out over a period of 8 weeks (one spermatogenic cycle). At the end of study, blood was collected for testosterone assay and the prostate was also harvested for histological procedure. Result: Result showed significant decrease in the prostatic weight of all the treated groups. However, testosterone significantly increased in group D and the histology revealed moderate to severe stroma fibrosis, high vascularization with vascular congestion which was due to severe infiltration of inflammatory cells in a dose dependent manner. Conclusion: Prolonged administration of exogenous oxytocin could led to a decrease in prostatic weight. Hence, clinician prescribing oxytocin for the treatment of oligozoospermia should be conscious of the risk of exogenous oxytocin in inducing prostatic disorders.

show abstract

Oxytocin differentially effects 3β-hydroxysteroid dehydrogenase and 5α-reductase activities in prostate cancer cell lines

Cited by 4 publications

References 43 publications

The function of oxytocin: a potential biomarker for prostate cancer diagnosis and promoter of prostate cancer

The function of oxytocin: a potential biomarker for prostate cancer diagnosis and promoter of prostate cancer

Oxytocin modulates steroidogenesis-associated genes and estradiol levels in the placenta

Alterations in the histology of the prostate of Sprague-Dawley rats treated with oxytocin

Contact Info

Product

Resources

About