Oxytocin receptor expression in stallion testes and epididymides

Jung, Youngwook; Yoon, Minjung

doi:10.1016/j.domaniend.2020.106562

Cited by 10 publications

(8 citation statements)

References 33 publications

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“…In pigs, an empirical study reported that supplementation of diluted semen with OXT did not influence sperm motility or the integrity of plasma and acrosomal membranes in both fresh and frozen-thawed sperm [ 29 ]. Although there is evidence that mammalian sperm cells have OXTR (stallion: [ 40 ]), suggesting that SP-OXT could modulate sperm functionality, our results and the aforementioned studies would indicate otherwise, by less seminal OXT would not a major influence in sperm parameters as relevant as motility, membrane integrity or lipid peroxidation. Perhaps seminal OXT may influence other sperm functions, such as sperm capacitation.…”

Section: Discussioncontrasting

confidence: 52%

“…Focusing on male pigs, the OXT concentrations measured in SP in this study were higher than those previously reported in saliva samples also collected from boars used in AI programs and measured using the same procedure [ 36 ]. This comparatively higher concentration of OXT in SP could suggest an eventual functional role in either sperm or on the female genital tract, since both in sperm (demonstrated in stallions: [ 40 ]) and in the endometrium and myometrium (demonstrated in sows: [ 41 ]) there are OXTRs. Noteworthy, the measured concentrations of OXT in single SP samples (one ejaculate per boar) varied between boars but not between breeds.…”

Section: Discussionmentioning

confidence: 99%

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Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows

Padilla

López-Arjona

Martı́nez-Subiela

et al. 2021

J Animal Sci Biotechnol

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Background Identification of relevant in vivo biomarkers for fertility remains a challenge for the livestock industry. Concentrations of the small peptide hormone oxytocin (OXT), involved in male reproductive function and present in the seminal plasma (SP) of several species could be a robust one. This study characterized concentrations of SP-OXT in ejaculates from boars used in artificial insemination (AI) programs aiming to evaluate its relationship with sperm quality variables and in vivo fertility of their liquid-stored AI-semen. Seminal OXT concentrations (ng/mL) were measured in 169 ejaculates from 61 boars of the Duroc, Pietrain, Landrace and Large White breeds using a direct competitive immunoassay test based on AlphaLISA® technology. Ejaculate (ejaculate volume, sperm concentration, total sperm count) and sperm parameters (motility, viability, intracellular generation of reactive oxygen species, plasma membrane fluidity) were assessed at 0 h and 72 h in AI-semen samples stored at 17 °C. In vivo fertility included only 18 Large White and Landrace boars whose AI-semen was used to inseminated > 100 sows and evaluated both farrowing rate and litter size of 3,167 sows. Results The results showed that SP-OXT differed between boars and between ejaculates within boar (P < 0.05) but not between breeds (Duroc, Pietrain, Landrace and Large White). Ejaculates with higher SP-OXT concentration/mL (hierarchically grouped; P < 0.001) had larger volume and came from younger boars (P < 0.05). Ejaculates of boars showing positive farrowing rate deviation exhibited higher (P < 0.05) SP-OXT concentration/mL than those with negative farrowing rate deviation. Conclusion The SP concentrations of OXT are boar, ejaculate and age dependent, and positively related with ejaculate volume and farrowing rates of liquid-stored semen AI-doses.

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Section: Discussioncontrasting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows

Padilla

López-Arjona

Martı́nez-Subiela

et al. 2021

J Animal Sci Biotechnol

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“…Within the convoluted seminiferous tubules, OTRs are expressed in spermatogenic cells, in particular round and elongated spermatids (Figure 1). However, the expression of these receptors was not detected in Sertoli cells of tom cats as found in stallions (Jung & Yoon, 2021). These findings are contradictory to previous reports in other species including humans (Frayne & Nicholson, 1998), rams (Whittington et al., 2001) and dogs (Prapaiwan et al., 2017).…”

Section: Discussionmentioning

confidence: 97%

“…The presence and localization of these receptors in the testis and efferent ducts reflect the essential roles of oxytocin in spermatogenesis (Anjum et al., 2018) and sperm transport (Frayne et al., 1996; Kunz et al., 2007; Thackare et al., 2006). Immunohistochemical localization of OTRs in reproductive tracts of male animals has been revealed in several studies such as in mice (Ang et al., 1991; Assinder et al., 2002), rats (Foo et al., 1991), rams (Assinder et al., 2000), dogs (Dalmazzo et al., 2019) and stallions (Jung & Yoon, 2021). However, differences in terms of localization and functions have consistently been reported among species.…”

Section: Discussionmentioning

confidence: 99%

Expression of oxytocin receptors and oxytocin assisted electroejaculation in the domestic cat (Felis catus)

Tiptanavattana

Pakdeesanaeha

Thongsima

et al. 2022

Reprod Domestic Animals

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Oxytocin is a peptide hormone that mainly functions to control the contractility of smooth muscles and sex‐related steroidogenesis in male reproductive tracts. However, specific information concerning this hormone in controlling the reproductive organs of cats is limited. This study aimed to investigate the expression of oxytocin receptors (OTRs) and their signal mediator via prostacyclin synthase (PTGIS) in reproductive structures following oxytocin assisted electroejaculation. In Experiment 1, the testis, cauda epididymis and vas deferens from five cats were examined by immunohistochemistry and quantitative polymerase chain reaction in order to study the responses of OTR and PTGIS mRNA to oxytocin injection. Experiment 2 examined the effect of oxytocin administration prior to electroejaculation on ejaculate characteristics and sperm quality in terms of motility, viability and fertilizing ability. Immunohistochemistry revealed the expression of OTRs in Leydig's, peritubular myoid cells and some spermatogenic cells. The expression was found in the epithelium and smooth muscle of the epididymis and vas deferens. After oxytocin administration, the OTR mRNA was upregulated in the epididymis (p > .05) and vas deferens (p = .01). The expression level of PTGIS mRNA increased in the response to oxytocin treatment only for the vas deferens (p > .05). Oxytocin treatment before electroejaculation resulted in an approximately twofold increase in sperm concentration and total sperm output/ejaculate, while this intervention did not significantly affect ejaculate volume, sperm quality or fertilizing ability. This study concluded that the oxytocin cascade is locally present in the reproductive structures and plays a role in promoting sperm delivery during electroejaculation in cats.

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“…This can be explained considering that oxytocin has a direct modulatory effect on testicular function, especially testosterone production within the Leydig cells. Several studies recorded the presence of oxytocin receptors on the Leydig and Sertoli cells (Jung & Yoon, 2020; Whittington et al., 2001). Previous studies reported that oxytocin inhibits testosterone biosynthesis in isolated rat Leydig cells.…”

Section: Discussionmentioning

confidence: 99%

Exogenous oxytocin administration improves the testicular blood flow in rams

El-Shalofy

Hedia

2021

Andrologia

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The current research aimed to examine the effects of single-dose oxytocin administration on testicular blood flow measurements peak systolic velocity (PSV), enddiastolic velocity (EDV), resistive index (RI) and pulsatility index (PI) and plasma steroid (testosterone and oestradiol-17β) concentrations in rams. In the treated group, eight How to cite this article: El-Shalofy, A. S., & Hedia, M. G. (2021). Exogenous oxytocin administration improves the testicular blood flow in rams. Andrologia, 53, e14193.

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Oxytocin receptor expression in stallion testes and epididymides

Cited by 10 publications

References 33 publications

Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows

Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows

Expression of oxytocin receptors and oxytocin assisted electroejaculation in the domestic cat (Felis catus)

Exogenous oxytocin administration improves the testicular blood flow in rams

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