Oxytocin receptor in human fetal membranes at term and during labor

Benedetto, M. T.; Cicco, Fiorenzo De; Rossiello, F.; Nicosia, Antonella; Lupi, Giulia; Dell'Acqua, S

doi:10.1016/0022-4731(90)90276-x

Cited by 41 publications

(13 citation statements)

References 16 publications

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“…The expression of OTR mRNA and protein in the amnion, the other fetally derived tissue at the feto-maternal interface, seems much lower than that of trophoblasts in the chorion laeve. In the previous studies, some investigators demonstrated the plenty amount of OT binding site in the amnion (13,14). However, our results are reproducible, and A.-R. Fuchs also demonstrated only trace amount of OTR in the human amnion, using a binding assay (personal communication).…”

Section: Discussionsupporting

confidence: 77%

Expression and localization of human oxytocin receptor mRNA and its protein in chorion and decidua during parturition.

Takemura¹,

Kimura²,

Nomura³

et al. 1994

J. Clin. Invest.

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Section: Discussionsupporting

confidence: 77%

Expression and localization of human oxytocin receptor mRNA and its protein in chorion and decidua during parturition.

Takemura¹,

Kimura²,

Nomura³

et al. 1994

J. Clin. Invest.

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“…In humans, oxytocin stimulates the production of PGF2, by decidua in vitro (20,21). Receptors for oxytocin have also been found in human amnion and chorion but there is conflicting evidence on whether receptors increase in relation to labor (22,23). Oxytocin mRNA in chorio-decidual tissue is present in three to fourfold higher concentrations after vaginal delivery than before the onset of labor, suggesting an autocrine or paracrine role for oxytocin in labor (24).…”

Section: Introductionmentioning

confidence: 99%

The effect of oxytocin receptor blockade on parturition in guinea pigs.

Schellenberg¹

1995

J. Clin. Invest.

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The mechanism of the onset of labor is unknown in humans and guinea pigs. Contrary to most other species, progesterone withdrawal appears not to precede the onset of labor. To elucidate the role of oxytocin in the onset and maintenance of labor, guinea pigs were fitted with vascular catheters, an intraabdominal pressure catheter and an array of uterine electromyogram electrodes. An oxytocin antagonist '-oxytocin, 20 ,ug/kg per h, n = 11) or saline solution (n = 12) was infused starting on day 66 of gestation (term is 69 d). Oxytocin receptor blockade resulted in decreased uterine activity and a prolonged expulsive phase (second stage) of labor. Fetal delivery was delayed and fetal mortality was increased. The onset of the expulsive phase of labor was delayed but maximum uterine activity occurred in time together with a timely change in uterine electromyogram activity from a prepartumr to a postpartum pattern following an unaltered progressive increase in baseline uterine activity. This indicates that oxytocin is requisite for the normal progress of the first and second stage of labor, but has no involvement in the mechanism of the onset and the timing of labor. (J. Clin. Invest. 1995. 95:13-19.)

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“…In contrast, the primary site of OXT induction of prostaglandin synthesis in humans is the decidua [12]. The extraordinary upregulation of OXTRs in the amnion appears to be unique to the rabbit, as the increase in OXTR in human amnion at the end of pregnancy is only about 1% or less of that seen in the rabbit [10,11], and there are no OXTRs apparent in the rat amnion [21]. However, irrespective of the tissue source of OXT-stimulated prostaglandin synthesis, it has been repeatedly demonstrated that elevated prostaglandin synthesis is associated with the onset of labor.…”

Section: Discussionmentioning

confidence: 73%

“…Cultured human amnion cells increase synthesis of prostaglandin E 2 (PGE 2 ) in response to various agents [7,8], including OXT [9]. However, the concentration of OXTR in human amnion is only marginally elevated at the end of pregnancy [10,11] in comparison with the decidua [6,12], suggesting that the decidua is the major target of OXTR-stimulated prostaglandin release in humans. In contrast to these findings, there is only a 2-fold increase in decidual OXTR concentrations in the rabbit at the end of pregnancy compared with an abrupt 200-fold rise in the amnion [13].…”

Section: Introductionmentioning

confidence: 96%

Characterization of the Cyclic Adenosine Monophosphate Target Site in the Oxytocin Receptor Gene in Rabbit Amnion1

Jeng

Soloff

2009

Biology of Reproduction

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Oxytocin (OXT) is a potent stimulator of prostaglandin E(2) (PGE(2)) synthesis by rabbit amnion cells obtained near the end of pregnancy. Coincident with a marked increase in sensitivity of PGE(2) synthesis to OXT, the concentration of OXT receptors (OXTRs) is abruptly upregulated about 200-fold at term. This increase can be mimicked in preterm amnion cells in primary culture by the synergistic action of agents that increase cAMP synthesis and by glucocorticoids. To elucidate the mechanism of cAMP action, we cloned the rabbit OXTR gene and isolated a 200-base pair (bp) forskolin-responsive region about 4.7 kilobase upstream from the transcriptional start site using transient transfection assays. This region corresponds to a DNase I-hypersensitive site that appears in amnion tissue only near the end of pregnancy, when OXTRs are upregulated. The effects of forskolin were mediated in part by cAMP response element binding protein (CREB), as coexpression of reporter constructs with dominant negative CREB inhibited reporter expression. In addition, CREB was cross-linked to sites in the 200-bp region only in chromatin isolated from cells near the end of pregnancy, as demonstrated by chromatin immunoprecipitation (ChIP). Because the transient transfection results are consistent with work using tissue extracts (DNase I hypersensitivity and ChIP), we conclude that cAMP, acting through a specific upstream CREB binding site, is critical for the physiological upregulation of OXTRs in the amnion at the end of gestation.

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Oxytocin receptor in human fetal membranes at term and during labor

Cited by 41 publications

References 16 publications

Expression and localization of human oxytocin receptor mRNA and its protein in chorion and decidua during parturition.

Expression and localization of human oxytocin receptor mRNA and its protein in chorion and decidua during parturition.

The effect of oxytocin receptor blockade on parturition in guinea pigs.

Characterization of the Cyclic Adenosine Monophosphate Target Site in the Oxytocin Receptor Gene in Rabbit Amnion1

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