Oyster extracts attenuate pathological changes in non-alcoholic steatohepatitis (NASH) mouse model

Watanabe, Mamoru; Fuda, Hirotoshi; Okabe, Hiroaki; Joko, Sae; Miura, Y.; Hui, Shu‐Ping; Yimin,; Hamaoka, Naohiro; Miki, Emiko; Chiba, Hitoshi

doi:10.1016/j.jff.2015.11.029

Cited by 17 publications

(16 citation statements)

References 69 publications

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“…Taurine is one of the predominant amino acids in muscle tissue, plays an important role in battling oxidative stress-induced inflammation [29]. As the major amino acid of oyster extracts, taurine attenuated non-alcoholic steatohepatitis (NASH) mouse [30]. Similarly, ornithine has long been claimed to be beneficial for liver [6], promote the secretion of the growth hormone and muscle building.…”

Section: Composition Analysis Of Inpsmentioning

confidence: 99%

Identification of protein-polysaccharide nanoparticles carrying hepatoprotective bioactives in freshwater clam (Corbicula fluminea Muller) soup

Gao

Wang

et al. 2020

International Journal of Biological Macromolecules

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Bioactives can impact food function either by their dosage or by their forms of dispersion, though the latter remains mostly neglected. Here we report the incidental nanoparticles (iNPs) carrying hepatoprotective bioactives identified in freshwater clam (Corbicula fluminea Muller) soup, which is a folk remedy for liver conditions in East Asia. The soup was fractionated into two iNPs containing fractions with high yield (95.8%) in 35 min by gel chromatography. With hydrodynamic diameter (Dh) range from 40 nm to 149 nm, iNPs were mainly constituted by carbohydrates and proteins. Notably, the majority of bioactives, e.g. taurine (63.2%), ornithine (68.1%) and phytosterols (60.0%), was determined to be carried by the iNPs. It suggested a possible mechanism of elevated delivery and absorption of bioactives, explaining why the clam soup can work at the bioactive concentrations way lower than the individual compound. These iNPs have great potential to be developed into a functional food with most potent nutraceutical effects.

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Section: Composition Analysis Of Inpsmentioning

confidence: 99%

Identification of protein-polysaccharide nanoparticles carrying hepatoprotective bioactives in freshwater clam (Corbicula fluminea Muller) soup

Gao

Wang

et al. 2020

International Journal of Biological Macromolecules

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“…DHMBA has been reported to protect hepatocytes in vitro from oxidative stress, 40 and improve pathological and metabolic changes in the liver of NASH model mice. 28 The increased expression of quinone reductase and glutathione reductase, induced via activation of Nrf2 pathway by sulforaphane, are reported to maintain for more than 120 hours. 41 Thus, in our present study, DHMBA reduced the degree of oxidation in LDs possibly through the activation of Nrf2-pathway (Figs.…”

Section: Evaluation Of the Effects Of Food-derived Antioxidants On Ldmentioning

confidence: 99%

Novel Fluorescence-Based Method To Characterize the Antioxidative Effects of Food Metabolites on Lipid Droplets in Cultured Hepatocytes

Tsukui

Chen

Fuda

et al. 2019

J. Agric. Food Chem.

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A fluorescence microscopic method for characterizing size, quantity, and oxidation of lipid droplets (LDs) in HepG2 cells was developed. LDs were induced by palmitic (PA), oleic (OA), or linoleic acids (LA), and stained with two fluorescent probes for neutral lipids and lipid peroxides. Each fatty acid increased the number of LDs and oxidized LDs (oxLDs) and the degree of LD oxidation time-dependently, as well as increased intracellular triglyceride hydroperoxides. LDs induced by LA without AAPH showed the most significant oxidation degree over PA and OA, especially in large LDs (area ≥3 µm 2 , oxLD/LD = 52.3±21.7%). Under this condition, two food-derived antioxidants were evaluated, and both of them significantly improved the LD characteristics. Moreover, chlorogenic acid reduced the quantity of large LDs by 74.0%−87.6% dose-dependently. The proposed method might provide a new approach to evaluate the effect of dietary antioxidants.

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“…In a scatter plot showing the cytoprotective effect plotted against the log P for each antioxidant, most antioxidants were distributed along a single curve. However, sulforaphane and have been reported for sulforaphane 30,31 , and anti-non-alcoholic steatohepatitis (NASH) effects have been reported for DHMBA 32 . On the basis of the above evidence, flazin and flazin-rich foods may also have a promising future as functional foods.…”

Section: Alcohol Of the Flazin Analogues Might Easily Form R-c(o)-nh-mentioning

confidence: 99%

Flazin as a Promising Nrf2 Pathway Activator

Fuda

Miyanaga

Furukawa

et al. 2019

J. Agric. Food Chem.

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Flazin is a β-carboline-derived alkaloid found in Japanese fermented foods. Here, the potential of flazin as an antioxidant food was studied with particular regard to its effect on the Keap1-Nrf2 system in human hepatocytes (C3A). Flazin, flazin analogues, and the decarboxylated derivative perlolyrine were chemically synthesized and compared with each other and with chlorogenic acid and curcumin. Among these compounds, flazin showed the lowest cytotoxicity (IC50<500 µM) and the highest capacity to activate Keap1-Nrf2 and provided the most cytoprotection against a pro-oxidant, although its radical absorbance capacity was relatively low. Flazin increased Nrf2-dependent protein expression and Nrf2-dependent phase II enzyme gene expression. The characteristics of flazin, including its combination of strong cytoprotective ability and a low log P, are shared by sulforaphane and 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), suggesting the potential value of flazin and flazin-rich foods for the prevention and/or alleviation of health disorders involving oxidation processes.

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Oyster extracts attenuate pathological changes in non-alcoholic steatohepatitis (NASH) mouse model

Cited by 17 publications

References 69 publications

Identification of protein-polysaccharide nanoparticles carrying hepatoprotective bioactives in freshwater clam (Corbicula fluminea Muller) soup

Identification of protein-polysaccharide nanoparticles carrying hepatoprotective bioactives in freshwater clam (Corbicula fluminea Muller) soup

Novel Fluorescence-Based Method To Characterize the Antioxidative Effects of Food Metabolites on Lipid Droplets in Cultured Hepatocytes

Flazin as a Promising Nrf2 Pathway Activator

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