Ozonated autohemotherapy modulates the serum levels of inflammatory cytokines in gouty patients

Abstract

ObjectivesOzonated autohemotherapy (O3-AHT) has been used to effectively treat gout, but the underlying therapeutic mechanisms remain unknown. In this study, as an initial effort to understand the therapeutic mechanisms of O3-AHT, we aim to examine the effect of O3-AHT on serum inflammatory cytokine levels in gouty patients.Patients and methodsThree groups of patients and healthy subjects were recruited, including the gouty (n=10), hyperuricemia (n=10), and healthy control (n=11) groups. Cytometric bead array was applied to examine 12 cytokines before (T0), during (T1), and after (T2) therapies.ResultsThree cytokines, IL-8, IL-12, and MCP-1, were detectable in all participants. Before O3-AHT, the average serum levels of IL-8 and MCP-1 were higher in the gout group than in the hyperuricemia and healthy control groups, confirming the inflammation status in gouty patients. After the 5th course of O3-AHT (T1), IL-8 level was significantly increased compared to that at T0. IL-12 level was also raised at T1, although the difference did not reach statistical significance. After completing the therapy, both IL-8 and IL-12 levels decreased to levels lower than those at T0. MCP-1 level remained essentially unchanged during and after treatment.ConclusionOur results indicate that O3-AHT induces a significant change in serum cytokine levels, suggesting that modulating the inflammatory process is one of the therapeutic mechanisms underlying O3-AHT. In addition, the sensitive response of serum IL-8 and IL-12 levels to O3-AHT suggests that these cytokines may be developed as biomarkers to evaluate the therapeutic effect of O3-AHT in gouty patients.

Ozonated autohemotherapy combined with pulsed radiofrequency in the treatment of thoracic postherpetic neuralgia in older adults: a retrospective study

“… 16 The latter is related to an anti-inflammatory and anti-oxidant effect. 28 Li et al 29 demonstrated that O 3 -AHT alleviates inflammation by regulating the expression of crucial inflammation cytokines interleukin-8 and interleukin-12. Chang et al 30 delivered 3% and 5% ozone to the vessels of mice in the localized area, and then they discovered that this method can decrease the level of interleukin-1β, tumor necrosis factor-α, and interleukin-6.…”

Expert consensus of Chinese Association for the Study of Pain on the application of ozone therapy in pain medicine

“…Indications for ozone therapy are as follows: (1) Neuropathic pain: Herpes, postherpetic neuralgia and central pain[ 22 ], syringomyelia, diabetic peripheral neuropathy[ 23 ] and central and peripheral nerve injury pain[ 24 ]; (2) Vasogenic pain: diabetes and peripheral vascular disease[ 25 ], thrombotic ischemic pain[ 3 , 26 , 27 ], Raynaud’s disease, erythromelalgia and vasculitis[ 28 - 30 ]; (3) Metabolic immune diseases: Ankylosing spondylitis, rheumatoid arthritis[ 31 ], allergic diseases and gout[ 32 , 33 ]; (4) Infectious diseases: Necrotizing ulcers, hard to heal wounds[ 34 , 35 ] and burns; (5) Physiological pain: Dysmenorrhea; (6) Tumor pain: Tumor pain during adjuvant therapy, radiotherapy and chemotherapy side effects, tumor consumption treatment and cancerous neuralgia[ 36 ]; and (7) Degenerative spinal diseases and joint and skeletal muscle diseases: Discogenic low back pain, lumbar disc herniation, cervical spondylosis, knee osteoarthritis, hip osteoarthritis and pain caused by chronic muscle, tendon, ligament, fascia and joint capsule strain[ 37 ].…”

Ozone Improves Oxygenation and Offers Organ Protection after Autologous Blood Transfusion in a Simulated Carbon Dioxide Pneumoperitoneal Environment in a Rabbit Hemorrhagic Shock Model

“…It can increase the content of ATP and 2,3-DPG through the activation of phosphofructokinase [22], enhance the oxygen supply of blood to hypoxic tissues, and play an anti-inflammatory role by reducing the production of inflammatory factors such as TNF-α, IL-1β, and IL-6 [22]. O 3 also contributes to the treatment of organ ischemia-reperfusion injury [22], septic shock [23], acute cerebral infarction [24], gout [25], and other diseases. Therefore, we assumed that O 3 treatment might improve the oxygen-carrying capacity of RBCs, reduce oxidative stress after an autologous blood transfusion during laparoscopic surgery, reduce inflammation, and offer organ protection.…”