Ozone pretreatment of lignocellulosic materials for ethanol production: Improvement of enzymatic susceptibility of softwood

Abstract

In order to utilize lignocellulosic biomass as a feedstock for bioethanol, ozone pretreatment was conducted on Japanese cedar sawdust and three other lignocellulosic wastes. Successful lignin degradation was accomplished by ozone pretreatment of the Japanese cedar sawdust and over 90% of polysaccharides were converted to monomeric sugars by enzymatic saccharification. This ozone pretreatment was also effective with other lignocellulosics, such as Hinoki cypress sawdust, lumber and board wastes. Ethanol production by simultaneous saccharification and fermentation of the ozone pretreated Japanese cedar sawdust was also successful. It was shown that ozone pretreatment increases enzymatic susceptibility and enables the production of ethanol from lignocellulosic biomass.

Plasma-Assisted Pretreatment of Wheat Straw for Ethanol Production

“…Especially, hemicelluloses were highly conserved [4], which potentially can lead to a higher ethanol yield or alternatively, to C-5 derived packaging materials [25]. Sugimoto et al [24] achieved only 20% of the theoretically possible amount of ethanol, while we found an ethanol yield of 52%. A yield of 52% equals the theoretically expected yield from glucose taking the consumption of sugars for the metabolism of the yeast into account [26].…”

“…Sugimoto and coworkers [24] was the first to publish results on the subsequent ethanol production. However, the results presented here represent progress in a number of ways.…”

Ozone oxidation pretreatment for enzymatic saccharification of spent culture media after Lentinula edodes cultivation

“…On the other hand, the 80 % MC sample showed almost no changes in Klason lignin after ozone treatment. It is known that the MC of the sample affects the ozonolysis of lignocellulose [16,17,22]. The optimal MC of sample for ozone treatment is different by type and condition of sample [16,17].…”

“…It is known that the MC of the sample affects the ozonolysis of lignocellulose [16,17,22]. The optimal MC of sample for ozone treatment is different by type and condition of sample [16,17]. It is considered, therefore, that the optimal MC condition of sample for degrading the lignin by ozone treatment depends on the type of material.…”

“…Ozone has been applied as a pretreatment method for enzymatic saccharification of lignocellulosic biomass, such as herbaceous plants, wood, and SCM [15][16][17][18]. Sugimoto et al [17] treated Japanese cedar (Cryptomeria japonica) sawdust with ozone, resulting in an increase in sugar yield by enzymatic saccharification associated with an increase in lignin degradation by the ozone pretreatment. However, the effects of ozone pretreatment of SCM from L. edodes have not been intensively investigated.…”