Ozone Selectively Inhibits Growth of Human Cancer Cells

Sweet, Frederick; Kao, Ming‐Shian; Lee, Song-Chiau D.; Hagar, Will L.; Sweet, Wileen E.

doi:10.1126/science.7403859

Cited by 84 publications

(58 citation statements)

References 12 publications

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“…We did not see significant change in cell death in control culture which is not treated with ozone. Similar kind of response was observed in human cancer cell treated with ozone where treatment of ozone caused 40-60% cell death [8].…”

Section: Discussionsupporting

confidence: 62%

“…In 1980 laboratory studies by main stream cancer researchers at Washington University discovered that the growth of human cancer cells from lung, breast, and uterine tumors was selectively inhibited in a dosedependent manner by ozone at 0.3 to 0.8 part per million of ozone in ambient air during 8 days of culture. Human lung diploid fibroblasts served as noncancerous control cells [8]. In 1990 preclinical French studies reported ozone enhanced the treatment of chemo resistant tumors and seemed to act adjunctively to chemotherapy in tumors derived from the colon and breast [9].…”

Section: Discussionmentioning

confidence: 99%

“…As far as role of ozone in cancer treatment is concerned, there are quite a few investigations done which suggests that ozone has beneficial effects in cancer. In 1980 laboratory studies by main stream cancer researchers at Washington University discovered ozone inhibited growth of lung, breast, and uterine cancer cells in a dose dependent manner while healthy tissues were not damaged by ozone [8]. Although these preliminary findings have long been argued justified funding for further research it should not be assumed that ozone would be similarly effective in vivo and could cure cancer.…”

Section: Introductionmentioning

confidence: 99%

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Ozone Induced Cell Death in HeLa cell Culture Mediated through Stimulation of TNF- Alpha

Khan¹

2015

MOJI

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Ozone has been in used as a therapeutic agent for the treatment of infective and inflammatory diseases, including cancer. The relationship of ozone with cancer and mechanism underlying the therapeutic effect of ozone on cancer remain unclear. This is first attempt to study the effect of ozone on cancer cells and how ozone induced cell death is mediated by activation of tumor necrosis factor (TNF)-α by using in vitro cell culture method. In the present study, HeLa cell cultures were subjected to ozone treatment at different dosages (10, 20, 30 & 40 µg/ml). HeLa cells exhibited an increasing cell death with increase in ozone doses studied. A substantial enhancement of cell death was observed after 18 hours of ozone treatment onwards. Treatment with 30 and 40 µg/ml of ozone showed an elevated sensitivity to growth inhibition compared to the treatments 10 and 20µg/ ml. Additionally cell detachment and cell floating were also faster and higher in ozone treatment with 30 and 40µg/ml. Tumor necrosis factor alpha (TNF-α), is a multifunctional cytokine playing a key role in apoptosis and cell survival as well as in inflammation and immunity. Hence we also studied the release of TNF-α in response to various dosages of ozone-treatments by using ELISA technique. We found that TNF-α, secretion was found to be enhanced with increase in cell death and at dose 40µg/ml ozone treatment, cell death was highest and TNF-α secretion was also highest. Our study suggests that ozone induced cell death in HeLa cell lines is mediated through the activation of TNF-α pathway and the role of ozone in inducing apoptosis cannot be ruled out.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ozone Induced Cell Death in HeLa cell Culture Mediated through Stimulation of TNF- Alpha

Khan¹

2015

MOJI

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“…Ozone, which can also produce oxygen free radicals, selectively inhibits growth of human cancer cells in comparison to normal cells [28]. Indeed, many of the anticancer drugs appear to generate the superoxide or hydroxyl radicals when activated appropriately .…”

Section: Discussionmentioning

confidence: 99%

The apparent production of superoxide and hydroxyl radicals by hematoporphyrin and light as seen by spin‐trapping

Buettner

Oberley

1980

FEBS Letters

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“…In 1980 Sweet et al presented the evidence of ozone inhibiting effect relative to tumor cells in vitro, having found a weak ability of these cells to compensate the oxygen "burst" caused by ozone in comparison with normal cells, and estimated 90% suppression of malignant cell growth [1].…”

mentioning

confidence: 99%

Effect of Ozone and Doxorubicin on the Viability and Morphology of Malignant Hepatic Cells

Alyasova¹,

Vedunova²,

Mishchenko³

et al. 2016

Sovrem Tehnol Med

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The aim of the investigation was to study the effect of isolated and combined action of ozone-oxygen mixture and doxorubicin on the viability and morphology of normal and malignant human hepatic cells.Material and Methods. The culture of normal (Chang liver) and malignant (SK-HEP-1) human liver cells were used in the study. Doxorubicin and ozone-oxygen gas mixture were introduced to the culture medium for cell growth. Viability was tested by reduction of MTT tetrazole salts. Morphological investigations were carried out 48 h after the replacement of the cultural medium using DMIL HC inverted microscope (Germany).Results. Doxorubicin possesses the highest cytotoxicity relative to both normal and malignant human hepatic cells. Ozone alone and in combination with doxorubicin also exerts a marked cytotoxic effect on the viability of the cells and results in irreversible consequences for the structure of the cell elements. The results obtained can be used for selection of ozone doses and ways of ozone introduction in various tumor localizations.

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Ozone Selectively Inhibits Growth of Human Cancer Cells

Cited by 84 publications

References 12 publications

Ozone Induced Cell Death in HeLa cell Culture Mediated through Stimulation of TNF- Alpha

Ozone Induced Cell Death in HeLa cell Culture Mediated through Stimulation of TNF- Alpha

The apparent production of superoxide and hydroxyl radicals by hematoporphyrin and light as seen by spin‐trapping

Effect of Ozone and Doxorubicin on the Viability and Morphology of Malignant Hepatic Cells

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