Ozone sensitivity of four Pakchoi cultivars with different leaf colors: physiological and biochemical mechanisms

Zhang, Lanwei; Song, Xiu-Neng; Chen, Y. J.; Xu, Hong; Li, Y. G.; Zhang, Y. W.; Luan, Feishi

doi:10.1007/s11099-016-0661-4

Cited by 18 publications

(14 citation statements)

References 49 publications

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“…In this study, we clearly observed visible foliar damage on the adaxial surface of the leaves of plants held under O and OT at both 7 and 14 DAE. Visible symptoms are regarded as important indicators of O 3 sensitivity in plants [38]. The first appearance of visible symptoms occurred at 3 DAE under OT and at 5 DAE under O.…”

Section: Discussionmentioning

confidence: 99%

Effects of Elevated Temperature and Ozone in Brassica juncea L.: Growth, Physiology, and ROS Accumulation

Lee

Woo

Kwak

et al. 2020

Forests

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Global warming and ozone (O3) pose serious threats to crop yield and ecosystem health. Although neither of these factors will act individually in reality, most studies have focused on the responses of plants to air pollution or climate change. Interactive effects of these remain poorly studied. Therefore, this study was conducted to assess the effects of optimal (22/20 °C day/night) and elevated temperature (27/25 °C) and/or ambient (10 ± 10 nL L−1) and elevated O3 concentrations (100 ± 10 nL L−1) on the growth, physiology, and reactive oxygen species (ROS) accumulation of leaf mustard (Brassica juncea L.). The aim was to examine whether elevated temperature increase the O3 damage due to increasing stomatal conductance, and thus, O3 flux into the leaf. Significant reductions in photosynthetic rates occurred under O (elevated O3 with optimal temperatures) and OT (elevated O3 and temperature) conditions compared to C (controls). Stomatal conductance was significantly higher under T than in the C at 7 DAE. Under OT conditions, O3 flux significantly increased compared to that in O conditions at 7 days after exposure (DAE). Significant reductions in total fresh and dry weight were observed under OT conditions compared to those under O. Furthermore, significant reductions in levels of carotenoids and ascorbic acid were observed under OT conditions compared to O. Lipid peroxidation and accumulation of ROS such as hydroxyl radical, hydrogen peroxide, and superoxide radical were higher under O and OT conditions than in C conditions at 7 and 14 DAE. As a result of O3 stress, the results of the present study indicated that the plant injury index significantly increased under OT compared to O conditions. This result suggested that elevated temperature (+5 °C) may enhance O3 damage to B. juncea by increasing stomatal conductance and O3 flux into leaves.

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Section: Discussionmentioning

confidence: 99%

Effects of Elevated Temperature and Ozone in Brassica juncea L.: Growth, Physiology, and ROS Accumulation

Lee

Woo

Kwak

et al. 2020

Forests

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“…Ozone pollution may cause serious injury to leafy vegetables and result in great losses of productivity and quality [ 16 ]. In our previous study, E-O 3 caused visible injury and physiological alterations in Pak Choi but the molecular mechanism was not clear [ 19 ]. Previous reports showed that high O 3 concentration triggers O 3 -responsive genes [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is noteworthy that the experiment was designed to simulate an acute O 3 exposure according to realistic O 3 concentrations in relatively clear area and in a highly O 3 -polluted area or a projected future [ 22 , 23 ]. Based on the results of our previous physiological study with this species [ 19 ], we postulated that 2 days were sufficient to analyze the O 3 -responsive genes in Pak Choi. The current results not only characterized the essential key genes, but also unveiled potential metabolic pathways or TFs that are potentially involved in cellular regulation or adaptation against O 3 stress.…”

Section: Discussionmentioning

confidence: 99%

“…chinensis L. Makino) is one of the most consumed vegetables in East Asia with high healthy and commercial values [ 18 ]. In our previous study, we found that Pak Choi is sensitive to acute O 3 fumigation as reflected by decreased chlorophyll content, increased anthocyanin content, damaged cell membrane integrity, enhanced antioxidative enzyme activities, depressed photosynthetic rate and stomatal conductance, inhibited maximal quantum yield and effective quantum yield of PSII photochemistry [ 19 ]. However, the underlying molecular mechanisms for such physiological alterations are not clear.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of Pak Choi under acute ozone exposure revealed regulatory mechanism against ozone stress

Zhang

et al. 2017

BMC Plant Biol

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BackgroundGround-level ozone (O3) is one of the major air pollutants, which cause oxidative injury to plants. The physiological and biochemical mechanisms underlying the responses of plants to O3 stress have been well investigated. However, there are limited reports about the molecular basis of plant responses to O3. In this study, a comparative transcriptomic analysis of Pak Choi (Brassica campestris ssp. chinensis) exposed to different O3 concentrations was conducted for the first time.ResultsSeedlings of Pak Choi with five leaves were exposed to non-filtered air (NF, 31 ppb) or elevated O3 (E-O3, 252 ppb) for 2 days (8 h per day, from 9:00–17:00). Compared with plants in the NF, a total of 675 differentially expressed genes (DEGs) were identified in plants under E-O3, including 219 DEGs with decreased expressions and 456 DEGs with increased expressions. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that O3 stress invoked multiple cellular defense pathways to mitigate the impaired cellular integrity and metabolism, including ‘glutathione metabolism’, ‘phenylpropanoid biosynthesis’, ‘sulfur metabolism’, ‘glucosinolate biosynthesis’, ‘cutin, suberine and wax biosynthesis’ and others. Transcription factors potentially involved in this cellular regulation were also found, such as AP2-ERF, WRKY, JAZ, MYB etc. Based on the RNA-Seq data and previous studies, a working model was proposed integrating O3 caused reactive oxygen burst, oxidation-reduction regulation, jasmonic acid and downstream functional genes for the regulation of cellular homeostasis after acute O3 stress.ConclusionThe present results provide a valuable insight into the molecular responses of Pak Choi to acute O3 stress and the specific DEGs revealed in this study could be used for further functional identification of key allelic genes determining the O3 sensitivity of Pak Choi.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1202-4) contains supplementary material, which is available to authorized users.

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“…Chlorophyll content was determined using a modified version of the method reported by [28]. Pak-choi tissue powder (1 g) was homogenized in 10 mL acetone: ethanol (2:1), then the mixture was centrifuged at 12,000× g for 10 min at 4 • C. The total chlorophyll content was determined by measuring sample absorbance at 645 nm and 663 nm.…”

Section: Chlorophyllmentioning

confidence: 99%

Integrated Analysis of Transcriptomic and Metabolomic Data Reveals the Mechanism by Which LED Light Irradiation Extends the Postharvest Quality of Pak-choi (Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee)

Yan¹,

Zuo²,

Zhou

et al. 2020

Biomolecules

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Low-intensity (10 μmol m−2 s−1) white LED (light-emitting diode) light effectively delayed senescence and maintained the quality of postharvest pakchoi during storage at 20 °C. To investigate the mechanism of LED treatment in maintaining the quality of pakchoi, metabolite profiles reported previously were complemented by transcriptomic profiling to provide greater information. A total of 7761 differentially expressed genes (DEGs) were identified in response to the LED irradiation of pak-choi during postharvest storage. Several pathways were markedly induced by LED irradiation, with photosynthesis being the most notable. More specifically, porphyrin and chlorophyll metabolism and glucosinolate biosynthesis were significantly induced by LED irradiation, which is consistent with metabolomics reported previously. Additionally, chlorophyllide a, chlorophyll, as well as total glucosinolate content was positively induced by LED irradiation. Overall, LED irradiation delayed the senescence of postharvest pak-choi mainly by activating photosynthesis, inducting glucosinolate biosynthesis, and inhibiting the down-regulation of porphyrin and chlorophyll metabolism pathways. The present study provides new insights into the effect and the underlying mechanism of LED irradiation on delaying the senescence of pak-choi. LED irradiation represents a useful approach for extending the shelf life of pak-choi.

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Ozone sensitivity of four Pakchoi cultivars with different leaf colors: physiological and biochemical mechanisms

Cited by 18 publications

References 49 publications

Effects of Elevated Temperature and Ozone in Brassica juncea L.: Growth, Physiology, and ROS Accumulation

Effects of Elevated Temperature and Ozone in Brassica juncea L.: Growth, Physiology, and ROS Accumulation

Transcriptomic analysis of Pak Choi under acute ozone exposure revealed regulatory mechanism against ozone stress

Integrated Analysis of Transcriptomic and Metabolomic Data Reveals the Mechanism by Which LED Light Irradiation Extends the Postharvest Quality of Pak-choi (Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee)

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