Ozone treatment of conditioned wastewater selects antibiotic resistance genes, opportunistic bacteria, and induce strong population shifts

Alexander, Johannes; Knopp, Gregor; Dötsch, Andreas; Wieland, Arne; Schwartz, Thomas

doi:10.1016/j.scitotenv.2016.03.154

Cited by 227 publications

(131 citation statements)

References 59 publications

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“…Some opportunistic pathogens are highly adaptable to stress situation and are able to activate effective stress responses to survive adverse growth conditions (nutrient limitation, low temperatures, etc. ; Alexander et al, 2015, 2016). Hence, these microorganisms are able to persist in uncomfortable situation and will not die.…”

Section: Discussionmentioning

confidence: 99%

“…Using these curves, the measured Ct values of the mcr-1 gene, the 16S ribosomal RNA gene or the taxon-specific gene markers from water samples can be used for copy number calculations (Alexander et al, 2015, 2016). The abundances of antibiotic resistance genes (ARGs) and opportunistic bacteria were quantified in each water sample and normalized to 100 ng total extracted DNA (cell equivalents per 100 ng DNA).…”

Section: Methodsmentioning

confidence: 99%

“…To minimize the leaking of antibiotics or antibiotic-resistant bacteria into the environment, the use of antibiotics in human and veterinary medicine needs to be reduced. Indications of the importance of sewage water and wastewater treatment plants (WWTP) for the spread of antibiotic-resistant pathogens were already described (Rizzo et al, 2013; Alexander et al, 2015, 2016). …”

Section: Introductionmentioning

confidence: 99%

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Occurrence of the mcr-1 Colistin Resistance Gene and other Clinically Relevant Antibiotic Resistance Genes in Microbial Populations at Different Municipal Wastewater Treatment Plants in Germany

et al. 2017

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Seven wastewater treatment plants (WWTPs) with different population equivalents and catchment areas were screened for the prevalence of the colistin resistance gene mcr-1 mediating resistance against last resort antibiotic polymyxin E. The abundance of the plasmid-associated mcr-1 gene in total microbial populations during water treatment processes was quantitatively analyzed by qPCR analyses. The presence of the colistin resistance gene was documented for all of the influent wastewater samples of the seven WWTPs. In some cases the mcr-1 resistance gene was also detected in effluent samples of the WWTPs after conventional treatment reaching the aquatic environment. In addition to the occurrence of mcr-1 gene, CTX-M-32, blaTEM, CTX-M, tetM, CMY-2, and ermB genes coding for clinically relevant antibiotic resistances were quantified in higher abundances in all WWTPs effluents. In parallel, the abundances of Acinetobacter baumannii, Klebsiella pneumoniae, and Escherichia coli were quantified via qPCR using specific taxonomic gene markers which were detected in all influent and effluent wastewaters in significant densities. Hence, opportunistic pathogens and clinically relevant antibiotic resistance genes in wastewaters of the analyzed WWTPs bear a risk of dissemination to the aquatic environment. Since many of the antibiotic resistance gene are associated with mobile genetic elements horizontal gene transfer during wastewater treatment can't be excluded.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence of the mcr-1 Colistin Resistance Gene and other Clinically Relevant Antibiotic Resistance Genes in Microbial Populations at Different Municipal Wastewater Treatment Plants in Germany

et al. 2017

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“…However, other research has observed higher survival rates of resistant strains compared to sensitive bacteria, selection of ARGs, and shifts in bacterial population in the effluent after advanced treatments (Lüddeke et al, 2015;McKinney and Pruden, 2012;Alexander et al, 2016;Hu et al, 2016). Variations in reports on the efficiency of advanced approaches to wastewater treatment in controlling AR may be due to underestimates of the roles of variable operating conditions.…”

mentioning

confidence: 99%

Antimicrobial Resistance Genes and Wastewater Treatment

Mohammadali

Davies

2017

Antimicrobial Resistance in Wastewater Treatment Processes

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“…For E. coli and some ozone tolerant bacteria, intracellular genes were disrupted at specific ozone doses. However, the study concluded that ozone doses commonly used for elimination of micro-pollutants from wastewater do not always remove intracellular ARGs.Impact of ozone on ARBs and ARGs wasinvestigated in an ARG abundance study(Alexander et al, 2016). A concentration of 1 g ozone per 1 g DOC was utilized to treat clarified wastewater.…”

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confidence: 99%

Antimicrobial Resistance in the Environment

Waseem

Williams

Stedtfeld

et al. 2017

Water Environment Research

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This review summarizes selected publications of 2016 with emphasis on occurrence and treatment of antibiotic resistance genes and bacteria in the aquatic environment and wastewater and drinking water treatment plants. The review is conducted with emphasis on fate, modeling, risk assessment and data analysis methodologies for characterizing abundance. After providing a brief introduction, the review is divided into the following four sections: i) Occurrence of AMR in the Environment, ii) Treatment Technologies for AMR, iii) Modeling of Fate, Risk, and Environmental Impact of AMR, and iv) ARG Databases and Pipelines.

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Ozone treatment of conditioned wastewater selects antibiotic resistance genes, opportunistic bacteria, and induce strong population shifts

Cited by 227 publications

References 59 publications

Occurrence of the mcr-1 Colistin Resistance Gene and other Clinically Relevant Antibiotic Resistance Genes in Microbial Populations at Different Municipal Wastewater Treatment Plants in Germany

Occurrence of the mcr-1 Colistin Resistance Gene and other Clinically Relevant Antibiotic Resistance Genes in Microbial Populations at Different Municipal Wastewater Treatment Plants in Germany

Antimicrobial Resistance Genes and Wastewater Treatment

Antimicrobial Resistance in the Environment

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