P-168. Analysis of aneuploidy in unfertilized ICSI oocytes with two rounds of triple FISH

Martini, Elena; Flaherty, Sean P.; Swann, Nicholas J.; Payne, Dianna; Matthews, C.A.

doi:10.1093/humrep/12.suppl_2.199-a

Cited by 2 publications

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“…This was a significant improvement on karyotyping of oocytes in terms of I we report the findings related to a loss (hypohaploidy) or gain (hyperhaploidy) of the single chromatids. Some efficiency and an improvement on other FISH techniques in terms of the number of chromosomes studied oocytes presented an abnormal number of chromosomes for only one of the six chromosomes analyzed, (16). A similar approach recently was described by Liu et al (28) for studying six different chromosomes while others had a complex situation (i.e., loss of one chromosome or chromatid but gain of another, loss in blastomeres from human embryos.…”

Section: Fish Protocolmentioning

confidence: 95%

“…Two rounds of FISH were performed on each oocyte RESULTS (16). The first round used probes for chromosomes 1, 13, and 21.…”

Section: Fish Protocolmentioning

confidence: 99%

“…We recently developed a method for studying chromosomal anoma-drops using fine-drawn glass pipettes (inner diameter slightly larger than the oocyte). Each oocyte was trans-lies in metaphase II oocytes using the spreading technique of Coonen et al (15) and a novel FISH procedure ferred into a drop of HEPES-HTF containing 0.1% human serum albumin (HSA), then into a drop of involving two triple-probe hybridizations to enable up to six chromosomes to be studied simultaneously (16).…”

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confidence: 99%

“…detailed in Martini et al (16). Briefly, oocytes were transferred using a fine glass pipette to Super Starfrost Plus slides (Maenzel Glaeser, Germany).…”

mentioning

confidence: 99%

“…The chromosome 18 probe was Slides were examined using a DMRBE fluorescence microscope (Leica, Wetzlar, Germany) equipped with labeled with FITC-12-dUTP (Boehringer-Mannheim), the chromosome 7 probe was labeled with TRITC-4-a triple band pass filter block containing FITC, TRITC, and DAPI filters. The stringent scoring criteria used dUTP (Amersham, Buckinghamshire, UK), and aliquots of the chromosome 1 and X probes were labeled have been described by Martini et al (16). Paired signals reflect the unique nature of oocyte chromo-with FITC-12-dUTP and TRITC-4-dUTP.…”

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confidence: 99%

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Martini

Flaherty

Swann

et al. 2000

Journal of Assisted Reproduction and Genetics

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Section: Fish Protocolmentioning

confidence: 95%

“…Two rounds of FISH were performed on each oocyte RESULTS (16). The first round used probes for chromosomes 1, 13, and 21.…”

Section: Fish Protocolmentioning

confidence: 99%

mentioning

confidence: 99%

“…detailed in Martini et al (16). Briefly, oocytes were transferred using a fine glass pipette to Super Starfrost Plus slides (Maenzel Glaeser, Germany).…”

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confidence: 99%

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confidence: 99%

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Martini

Flaherty

Swann

et al. 2000

Journal of Assisted Reproduction and Genetics

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Profil des anomalies non létales du tube neural à l’échographie anténatale à Abidjan

Admin,

Anhum KONAN,

Idrissa GARBA

et al. 2020

J Afr Imag Méd

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Objectives: To describe the non-lethal neural tube defects discovered on prenatal ultrasound in Abidjan.Method: A six-year, multicentre, retrospective study was conducted in Abidjan, in the radiology departments of the Yopougon University Hospital and 2 private health centres. All obstetric ultrasound reports were reviewed and cases of non-lethal neural tube defects were identified. Sociodemographic data, types of malformations, their locations and associated signs were analysed.Results: We reviewed 6714 obstetrical ultrasound reports. The average age of the patients was 23.6 years with the 20 to 25 year age group predominating. Ultrasound examinations were performed in the 2nd quarter in 49% of cases and in the 3rd quarter in 41.3% of cases.The prevalence of neural tube defects was 1.1% or 110 cases per 10,000 pregnancies. The non-lethal forms seen in 44.8% of cases included, in decreasing order of frequency, hydrocephalus (43%), meningoceles (33%) and spina bifida (24%). A hydramnios was associated in 73.3% of cases.Conclusion: Non-lethal neural tube defects are dominated by hydrocephalus, encephaloceles and spina bifida. The ultrasound doctor must systematically look for them, in our context, in the event of the discovery of hydramnios or in the event of progressive maternal infection by rubella, syphilis or toxoplasmosis pathogens. Résumé Objectifs : décrire les anomalies non létales du tube neural, découvertes à l’échographie prénatale à Abidjan pour contribuer à l’amélioration de leur prise en charge diagnostique et thérapeutique. Méthode : étude rétrospective, multicentrique, d’une durée de six ans qui s’est déroulée à Abidjan, dans les services de radiologie du centre hospitalier universitaire de Yopougon et de 2 centres privés de santé. Tous les comptes rendus d’échographie obstétricale ont été relus et les cas de malformations non létales du tube neural ont été recensés. Les données sociodémographiques, les types de malformations, leurs sièges et les signes associés ont été analysés. Résultats : nous avons relu 6714 comptes rendus d’échographie obstétricale. L’âge moyen des patientes était de 23,6 ans avec prédominance de la tranche d’âge de 20 à 25 ans. Les échographies ont été réalisées au 2ème trimestre dans 49% des cas et au 3ème trimestre dans 41,3% des cas. La prévalence des anomalies du tube neural était de 1,1% soit 110 cas pour 10 000 grossesses. Les formes non létales vues dans 44,8% des cas, comportaient par ordre de fréquence décroissante, les hydrocéphalies (43%), les méningo-encéphalocèles (33%) et les spina bifida (24%). Un hydramnios était associé dans 73,3 % des cas. Conclusion : Les anomalies non létales du tube neural n’étaient pas rares dans notre contexte.Leur prévalence est demeurée constante, et elles étaient encoredominées par les hydrocéphalies. Le médecin échographiste devrait systématiquement les rechercher, devant un hydramnios ou en cas d’infection maternelle évolutive par les pathogènes de la rubéole, la syphilis ou la toxoplasmose.

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P-168. Analysis of aneuploidy in unfertilized ICSI oocytes with two rounds of triple FISH

Cited by 2 publications

References 0 publications

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Profil des anomalies non létales du tube neural à l’échographie anténatale à Abidjan

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