P-Bodies: Composition, Properties, and Functions

Luo, Yang; Na, Zhenkun; Slavoff, Sarah A.

doi:10.1021/acs.biochem.7b01162

Cited by 471 publications

(459 citation statements)

References 83 publications

(183 reference statements)

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“…Although future work in the laboratory will now focused on defining the composition and organisation of the Hfq foci, we propose that, at least conceptually, the Hfq foci resemble liquid-liquid phase separated ribonucleoprotein granules similar to eukaryotic P bodies, which are cytoplasmic ribonucleoprotein complexes comprising of complex networks protein-RNA interactions [34][35][36] . Since the addition of hexanediol, which has been previously shown to disrupt liquid-liquid phase separated structures in eukaryotic cells 28 , also disrupts the Hfq foci, we envisage that the Hfq foci could have an analogues function and properties to eukaryotic P bodies in the adaptive response to N starvation.…”

Section: Discussionmentioning

confidence: 99%

The assembly of Hfq into foci-like structures in response to long-term nitrogen starvation inEscherichia coli

McQuail

Switzer

Burchell

et al. 2020

Preprint

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Hfq is an RNA-binding protein that is common to diverse bacterial lineages and has, amongst many, a key role in RNA metabolism. We reveal that Hfq is required by Escherichia coli to adapt to nitrogen (N) starvation. By using single molecule tracking photoactivated localisation microscopy imaging of individual Hfq molecules in live E. coli cells, we have uncovered an unusual behaviour of Hfq: We demonstrate that Hfq forms a distinct and reversible focus-like structure specifically in long-term N starved E. coli cells. We show that foci formation by Hfq is a constituent process of the adaptive response to N starvation and provide evidence which implies that the Hfq foci, analogous to processing (P) bodies of stressed eukaryotic cells, contribute to the management of cellular resources to allow E. coli cells to optimally adapt to long-term N starvation stress.

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Section: Discussionmentioning

confidence: 99%

The assembly of Hfq into foci-like structures in response to long-term nitrogen starvation inEscherichia coli

McQuail

Switzer

Burchell

et al. 2020

Preprint

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“…We previously reported disappearance of processing bodies (P-bodies) during oocyte growth 24 . P-bodies are cytoplasmic foci forming as liquid-liquid phase separation from proteins involved in RNA metabolism, miRNA effector complexes and their targets (reviewed in 53 ). We initially hypothesized that P-body disappearance may reflect inhibition of the miRNA pathway, possibly caused by some defect in formation of the effector complex.…”

Section: Discussionmentioning

confidence: 99%

Low miRNA abundance disables microRNA pathway in mammalian oocytes

Kataruka

Modrák

Kinterová

et al. 2019

Preprint

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MicroRNAs (miRNAs) are ubiquitous small RNAs, which guide post-transcriptional gene repression in countless biological processes. However, the miRNA pathway in mouse oocytes appears inactive and is dispensable for development. We report that miRNAs do not accumulate like maternal mRNAs during oocyte growth. The most abundant miRNAs total tens of thousands of molecules in fully-grown oocytes, a number similar to that observed in much smaller fibroblasts. The lack of miRNA accumulation acts like miRNA knock-down, where miRNAs can engage their targets but are not abundant enough to produce significant silencing effect. Injection of 100,000 miRNAs was sufficient to restore reporter repression in oocytes, confirming that miRNA inactivity primarily stems from low miRNA abundance and not from an active repression of the miRNA pathway per se. Similar situation was observed in rat, hamster, porcine, and bovine oocytes arguing that miRNA inactivity is not a mousespecific adaptation but a common mammalian oocyte feature.

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“…Numerous condensates exist in all eukaryotic cells with unique composition, localization and function (Figure and Table ). Condensates that participate in house‐keeping functions such as ribosome biogenesis and RNA processing are constitutively present in most cell types (Figure , brown condensates) . Others assemble under certain stimuli such as proteotoxic, genotoxic or metabolic stress (Figure , red condensates) .…”

Section: Biomolecular Condensates: Assembly Function and Regulationmentioning

confidence: 99%

Biomolecular condensates in neurodegeneration and cancer

Spannl

Tereshchenko

Mastromarco

et al. 2019

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The intracellular environment is partitioned into functionally distinct compartments containing specific sets of molecules and reactions. Biomolecular condensates, also referred to as membrane‐less organelles, are diverse and abundant cellular compartments that lack membranous enclosures. Molecules assemble into condensates by phase separation; multivalent weak interactions drive molecules to separate from their surroundings and concentrate in discrete locations. Biomolecular condensates exist in all eukaryotes and in some prokaryotes, and participate in various essential house‐keeping, stress‐response and cell type‐specific processes. An increasing number of recent studies link abnormal condensate formation, composition and material properties to a number of disease states. In this review, we discuss current knowledge and models describing the regulation of condensates and how they become dysregulated in neurodegeneration and cancer. Further research on the regulation of biomolecular phase separation will help us to better understand their role in cell physiology and disease.

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P-Bodies: Composition, Properties, and Functions

Cited by 471 publications

References 83 publications

The assembly of Hfq into foci-like structures in response to long-term nitrogen starvation inEscherichia coli

The assembly of Hfq into foci-like structures in response to long-term nitrogen starvation inEscherichia coli

Low miRNA abundance disables microRNA pathway in mammalian oocytes

Biomolecular condensates in neurodegeneration and cancer

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