P XIII.98 DNA adduct detection in the liver of the dragonnet Callionymus lyra and the flounder Platchthys flesus: A potential bioindicator of pollutant

Boillot, K.; Burgeot, Thierry; Arnould, J P; Pfohl‐Leszkowicz, Annie

doi:10.1016/s0027-5107(97)83018-7

Cited by 3 publications

(3 citation statements)

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“…results also are in accordance with previous works that showed, in suspended matter, concentrations of PCBs within the whole estuary and of PAHs at sites downstream Rouen were higher than the acceptable limit effect defined by the Ospar convention [34]. The potential mutagenicity of PAHs is evidenced further by the work of Boillot et al [35] who reported that flounder (Platichthys flesus) sampled in La Bouille had high amounts of DNA adducts in the liver. The biological responses of D. polymorpha living in the estuary are consistent with the potential (calculated) effects of their body burdens of contaminants.…”

Section: Discussionsupporting

confidence: 92%

A pollution‐monitoring pilot study involving contaminant and biomarker measurements in the Seine Estuary, France, using zebra mussels (Dreissena polymorpha)

Minier

Abarnou

Jaouen-Madoulet

et al. 2006

Enviro Toxic and Chemistry

100

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Abstract-Zebra mussel (Dreissena polymorpha) is an invasive species that has proliferated in European and North American rivers and lakes during the last century. In this study, D. polymorpha has been used to provide information on contamination levels and biological effects in the Seine Estuary (France). The bivalves accumulated polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) to a high degree with values reaching 800 ng/g dry weight for PCBs (sum of 20 congeners), and 1,000 ng/g dry weight of PAHs (sum of 14 compounds) in the whole body. These values are among the highest reported of PCBs and, to a lesser extent, of PAHs in other contaminated areas in the world. Toxic equivalent quantities of PCBs and PAHs detected in zebra mussels varied from 20 to 40 pg dioxin equivalents/g dry weight for PCBs and up to 120 ng benzo[a]pyrene equivalents/ g dry weight for PAHs, indicating a high potential risk for animals feeding on them. Biological impacts, such as altered condition index, decreased lysosomal stability, and high levels of multixenobiotic resistance (MXR) proteins also were detected in mussels living downstream of Rouen, the main city of the Seine Estuary. Taken together, these results indicate that the Seine Estuary is a heavily polluted area with the potential to cause deleterious health effects in some endogenous living organisms. This study also shows that chemical and biological measurements bring different but complementary results that can help diagnose environmental health.

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Section: Discussionsupporting

confidence: 92%

A pollution‐monitoring pilot study involving contaminant and biomarker measurements in the Seine Estuary, France, using zebra mussels (Dreissena polymorpha)

Minier

Abarnou

Jaouen-Madoulet

et al. 2006

Enviro Toxic and Chemistry

100

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“…The presence of a DNA adduct in a critical gene provides the potential for occurrence of a mutagenic event, resulting in subsequent alterations in gene expression and a loss of growth control (for a review see Poirier et al 2000). The detection of DNA adducts is widely used as biomarker of aquatic contamination (Burgeot et al 1996;Boillot et al 1997;Ericson et al 1998;Ericson and Larsson 2000;Harvey et al 1997;Kurelec et al 1989a,b;Lyons et al 1999aLyons et al ,b, 2000Stein et al 1994;Stephensen et al 2000;Varanasi et al 1989).…”

Section: Introductionmentioning

confidence: 99%

DNA adducts in fish following an oil spill exposure

Amat

Burgeot²,

Castegnaro

et al. 2006

Environ Chem Lett

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On 12 December 1999, one third of the load of the Erika tanker, amounting to about 10,000 t crude oil flowed into sea waters close to the French Atlantic Coast. This oil contained polycyclic aromatic compounds (PAC) that are known to be genotoxic. Genotoxic effects induce DNA adducts formation, which can thus be used as pollution biomarkers. Here, we assessed the genotoxic impact of the "Erika" oil spill by DNA adducts detection in the liver of immature fishes (Solea solea) from four locations of the French Brittany coasts. Two months after the spill, a high amount of DNA adducts was found in samples from all locations, amounting to 92-290 DNA adduct per 10 9 nucleotides. Then total DNA adduct levels decreased to reach about 50 adducts per 10 9 nucleotides nine months after the spill. In vitro experiments using human cell cultures and fish liver microsomes evidence the genotoxicity of the Erika fuel. They also prove the formation of reactive species able to create DNA adducts. Furthermore, in vitro and in vivo DNA adducts fingerprints are similar, thus confirming that DNA adducts are a result of the oil spill.

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“…DNA adducts are thus probably one of the most relevant indicators of genetic damage due to exposure to toxins. The detection of DNA adducts is widely used as biomarker of aquatic contamination (Dunn et al 1987;Kurelec et al 1989a;Varanasi et al 1989;Ray et al 1991;Stein et al 1994;French et al 1996;El Adlouni et al 1995;Harvey et al 1997;Pfau et al 1997;Burgeot et al 1996;Boillot et al 1997;Ericson et al 1998;Ericson and Lasson 2000;Lyons et al 1999Lyons et al , 2000Stephensen et al 2000). Recently, Rice et al (2000) used English Sole (Pleuronectes vetulus) to test the genotoxic impact of polluted sediment.…”

Section: Introductionmentioning

confidence: 99%

Genotoxic Impact of ‘Erika’ Petroleum Fuel on Liver of the Fish Solea Solea

Amat¹,

Burgeot²,

Castegnaro³

et al.

Environmental Chemistry

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On 12 December 1999, one third of the load of the tanker 'Erika' , amounting to about 10 000 t crude oil flowed into sea waters close to the French Atlantic Coast (Finistère region). The spilled oil was fuel No. 6, a heavy or residual fuel that is a complex mixture of polycyclic aromatic compounds (PAC) of high molecular weight. Some polycyclic aromatic compounds are genotzoxic substances that induce carcinogenic lesions in laboratory animals. DNA adducts, reflecting genotoxic effects, are used as biomarker of early pollution. In this study, the genotoxic impact of the 'Erika' oil was assessed by studying the presence of DNA adduct in the liver of immature fishes (Solea solea) from four locations of the French Brittany coasts, two, six and nine months after the disaster. Two months after the spill, a high amount of DNA adducts was found in samples from all locations (92 to 290 DNA adduct/10 9 nucleotides). DNA adducts were more persistent in the North than in the South of the affected French Brittany coasts. In September, no significant difference was observed between the locations. When incubated in presence of an 'Erika' fuel extract, DNA adduct patterns similar to those obtained from the liver of Solea solea liver are observed both, in cell culture (HePG2) and, in presence of fish liver microsomes.

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P XIII.98 DNA adduct detection in the liver of the dragonnet Callionymus lyra and the flounder Platchthys flesus: A potential bioindicator of pollutant

Cited by 3 publications

References 0 publications

A pollution‐monitoring pilot study involving contaminant and biomarker measurements in the Seine Estuary, France, using zebra mussels (Dreissena polymorpha)

A pollution‐monitoring pilot study involving contaminant and biomarker measurements in the Seine Estuary, France, using zebra mussels (Dreissena polymorpha)

DNA adducts in fish following an oil spill exposure

Genotoxic Impact of ‘Erika’ Petroleum Fuel on Liver of the Fish Solea Solea

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