P003 The Effect of Centella Asiatica on Inactive Frequency and Movement Distance in Insomnia Zebrafish (Danio rerio) Larvae Model

Afif, Zamroni; Santoso, Mochammad Istiadjid Eddy; Khotimah, Husnul; Satriotomo, I; Iskandar, Dheka Sapti; Hakimah, A; Andriani, Nurvia; Agustina, Kartika

doi:10.1093/sleepadvances/zpac029.076

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“…data supporting research results Effect of Centella asiatica ethanol extract on zebrafish larvae (Danio rerio) insomnia model through inhibition of Orexin, ERK, Akt and p38, DOI: 10.6084/m9.figshare.24078519. 20 The project contains the following basic data:…”

Section: Data Availabilitymentioning

confidence: 99%

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Effect of Centella asiatica ethanol extract on zebrafish larvae (Danio rerio) insomnia model through inhibition of Orexin, ERK, Akt and p38

Afif,

Eddy Santoso,

Nurdiana

et al. 2024

F1000Res

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Background: Insomnia is difficulty initiating or maintaining sleep for at least three nights a week or more and lasting for at least 3 months. One of the molecules that play a role in the circadian rhythm of arousal system is hypocretin/orexin. Orexin activates the p38-MAPK signaling pathway and increases phosphorylated ERK1/2 levels. Centella asiatica (CA) has a role in the signal work of the MAPK/ERK, Akt, and p38 path in many various diseases. Methods: The research method used is true laboratory experimental. The research approach used was randomized control group post-test only. Zebrafish embryos aged 0-7 dpf were used in this study. The treatment group consisted of 5 groups: normal, insomnia, insomnia + 2.5 μg/mL CA, insomnia + 5 μg/mL CA, and insomnia + 10 μg/mL CA. The locomotor motion of zebrafish larvae was observed using Basler cameras on days five-, six- and seven-day post fertilization (dpf), then analyzed by using Western Blot method. Results: The results proved that exposure to CA extract was able to reduce the expression of orexin (91963 ± 9129) and p38 (117425 ± 6398) as an arousal trigger in the sleep-wake cycle, with the most optimal concentration of CA 5 μg/mL. Exposure to CA extract was also able to reduce the expression of ERK (94795 ± 30830) and Akt (60113.5 ± 27833.5) with an optimum concentration of CA 2.5 μg/mL. Conclusion: Exposure to CA extract was able to improve the sleep activity of zebrafish larvae insomnia model by extending the total inactivity time (cumulative duration) and shortening the duration of first sleep (latency to first) in light and dark phases through inhibition of orexin, ERK, p38, and Akt.

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Section: Data Availabilitymentioning

confidence: 99%

“…Figshare: The ARRIVE Essential 10: author checklist -Effect of Centella asiatica ethanol extract on zebrafish larvae (Danio rerio) insomnia model through inhibition of Orexin, ERK, Akt and p38, DOI: 10.6084/m9.figshare.24078519. 20 https://figshare.com/articles/dataset/preview_ARRIVE_10_checklist_author/24079407…”

Section: Data Availabilitymentioning

confidence: 99%