P104 Anaeroplasma, a potential anti-inflammatory probiotic for the treatment of chronic intestinal inflammation

Beller, A. J.; Kruglov, AA; Goetze, V von; Hoffmann, Ute; Maier, Richard; Heiking, Kevin; Siegmund, Britta; Heinz, GA; Mashreghi, M-F; Radbruch, Andreas; Chang, H-D

doi:10.1136/annrheumdis-2018-ewrr2019.92

Cited by 22 publications

(22 citation statements)

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“…Taxonomic identification in this study was resolved only to the level of genus, as species identification of bacteria can be problematic. 114,115 Although some phyla 116,117 and genera 118,119 have been correlated with specific physiological responses, it is important to note that bacterial species function can vary within the same genus. [120][121][122][123][124] This makes it challenging to form conclusions about the roles of genera as a whole within the microbiota, as well as to reduce the function of the entire microbiota as a system-which contains trillions of microorganisms 32 -and characterize it by only a handful of community members.…”

Section: Discussionmentioning

confidence: 99%

A novel role for the pineal gland: Regulating seasonal shifts in the gut microbiota of Siberian hamsters

Shor

Brown

Freeman

2020

Journal of Pineal Research

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The gut microbiota plays a significant role in a variety of host behavioral and physiological processes. The mechanisms by which the gut microbiota and the host communicate are not fully resolved but include both humoral and direct neural signals. The composition of the microbiota is affected by internal (host) factors and external (environmental) factors. One such signal is photoperiod, which is represented endogenously by nocturnal pineal melatonin (MEL) secretion. Removal of the MEL signal via pinealectomy abolishes many seasonal responses to photoperiod. In Siberian hamsters (Phodopus sungorus), MEL drives robust seasonal shifts in physiology and behavior, such as immunity, stress, body mass, and aggression. While the profile of the gut microbiota also changes by season, it is unclear whether these changes are driven by pineal signals. We hypothesized that the pineal gland mediates seasonal alterations in the composition of the gut microbiota. To test this, we placed pinealectomized and intact hamsters into long or short photoperiods for 8 weeks, collected weekly fecal samples, and measured weekly food intake, testis volume, and body mass. We determined microbiota composition using 16S rRNA sequencing (Illumina MiSeq). We found significant effects of treatment and time on the abundances of numerous bacterial genera. We also found significant associations between individual OTU abundances and body mass, testis mass, and food intake, respectively. Finally, results indicate a relationship between overall community structure, and body and testis masses. These results firmly establish a role for the pineal gland in mediating seasonal alterations in the gut microbiota. Further, these results identify a novel neuroendocrine pathway by which a host regulates seasonal shifts in gut community composition, and indicates a relationship between seasonal changes in the gut microbiota and seasonal physiological adjustments.

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Section: Discussionmentioning

confidence: 99%

A novel role for the pineal gland: Regulating seasonal shifts in the gut microbiota of Siberian hamsters

Shor

Brown

Freeman

2020

Journal of Pineal Research

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“…The representative OTU sequences of Ruminococcaceae UCG 005 are similar to those of Sporobacter termitidis , a hydrogen‐consuming acetogen isolated from the termite gut (91%–92% sequence similarity, according to BLAST analysis; Grech‐Mora et al, ). Members of Anaeroplasma reportedly induce increases in levels of mucosal IgA (Beller et al, ). Erysipelotrichaceae UCG 004 belongs to the family Erysipelotrichaceae , which is reportedly associated with inflammation‐related gastrointestinal diseases (Chen, Liu, Ling, Tong, & Xiang, ).…”

Section: Discussionmentioning

confidence: 99%

Bacterial communities in the solid, liquid, dorsal, and ventral epithelium fractions of yak (Bos grunniens) rumen

Ren

Yan

et al. 2019

MicrobiologyOpen

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Yak (Bos grunniens) is an important and dominant livestock species in the challenging environment of the Qinghai-Tibetan Plateau. Rumen microbiota of the solid, liquid, and epithelium fractions play key roles in nutrient metabolism and contribute to host adaptation in ruminants. However, there is a little knowledge of the microbiota in these rumen fractions of yak. Therefore, we collected samples of solid, liquid, dorsal, and ventral epithelium fractions from five female yaks, then amplified bacterial 16S rRNA gene V4 regions and sequenced them using an Illumina MiSeq platform. Principal coordinates analysis detected significant differences in bacterial communities between the liquid, solid, and epithelium fractions, and between dorsal and ventral epithelium fractions. Rikenellaceae RC9, the families Lachnospiraceae and Ruminococcaceae, and Fibrobacter spp. were the abundant and enriched bacteria in solid fraction, while the genera Prevotella and Prevotellaceae UCG 003 were higher in the liquid fraction. Campylobacter spp.,Comamonas spp., Desulfovibrio spp., and Solobacterium spp. were significantly higher in dorsal epithelium, while Howardella spp., Prevotellaceae UCG 001, Ruminococcaceae UCG 005, and Treponema 2 were enriched in the ventral epithelium. Comparison of predictive functional profiles among the solid, liquid, and dorsal, and ventral epithelium fractions also revealed significant differences. Microbiota in the ventral fraction of yak rumen also significantly differ from reported microbiota of cattle. In conclusion, our results improve our knowledge of the taxonomic composition and roles of yak rumen microbiota. K E Y W O R D SBos grunniens, Campylobacter spp., dorsal and ventral epithelium, ecology niches, Howardella spp, rumen, solid and liquid

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“…The enrichments of Ruminiclostridium 5 and Rikenellaceae RC9 gut group were considered to be related to the lipid metabolism [34,35]. Furthermore, genus Ruminiclostridium 9 was positive to blood IgM level and colitis histological scores [36] and Anaeroplasma was supposed to be a potential anti-inflammatory probiotic for the treatment of chronic intestinal inflammation [37]. The researchers illustrated that, although the impact of MHO7 on these genera was not entirely consistent as reported but it mediated intake metabolism and inflammatory responses.…”

Section: Changes Of Gut Microbiome In Mice After Oral Mho7mentioning

confidence: 87%

Toxicity, Pharmacokinetics, and Gut Microbiome of Oral Administration of Sesterterpene MHO7 Derived from a Marine Fungus

Tian

Deng

et al. 2019

Marine Drugs

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Sesterterpene MHO7 derived from mangrove fungus is a novel estrogen receptor degrader for the treatment of breast cancer. To explore its safety and pharmacokinetics in vivo, Log P/D values, stability in simulated gastric/intestinal (SGF/SIF), toxicity, and pharmacokinetics studies were carried mainly by liquid chromatography technique coupled with tandem mass spectrometry (LC–MS/MS) method in mice, and the effect of MHO7 on mice gut microbiota at different time points was revealed by 16S rRNA sequencing. Log P/D values ranged 0.93–2.48, and the compound in SGF and SIF is stable under the concentration of 5 mM·L−1. The maximum tolerance dose (MTD) of oral administration in mice was 2400 mg·kg−1. The main pharmacokinetics parameters were as following: Cmax of 1.38 μg·mL−1, Tmax of 8 h, a half-life (t1/2) of 6.97 h, an apparent volume of mean residual time (MRT) of 8.76 h, and an area under the curve (AUC) of 10.50 h·μg·mL−1. MHO7 displayed a wide tissue distribution in mice, with most of the compound in liver (3.01 ± 1.53 μg·g−1) at 1 h, then in fat (5.20 ± 3.47 μg·g−1) at 4 h, and followed by reproductive organs with the concentrations of 23.90 ± 11.33 μg·g−1,13.69 ± 10.29 μg·g−1, 1.46 ± 1.23 μg·g−1, and 0.36 ± 0.46 μg·g−1 at 8, 12, 20 and 30 h, respectively. The most influenced genera of gut microbiome belonged to phylum Firmicutes (21 of 28), among which 18 genera originated from the order Clostridiales, class Clostridia, and families of Ruminococcaceae (11 of 18) and Lachnospiraceae (4 of 18). These results provide that MHO7 is suitable for oral administration in the treatment of breast cancer with the target organs of reproductive organs and regulation on Ruminococcaceae and Lachnospiraceae.

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P104 Anaeroplasma, a potential anti-inflammatory probiotic for the treatment of chronic intestinal inflammation

Cited by 22 publications

References 0 publications

A novel role for the pineal gland: Regulating seasonal shifts in the gut microbiota of Siberian hamsters

A novel role for the pineal gland: Regulating seasonal shifts in the gut microbiota of Siberian hamsters

Bacterial communities in the solid, liquid, dorsal, and ventral epithelium fractions of yak (Bos grunniens) rumen

Toxicity, Pharmacokinetics, and Gut Microbiome of Oral Administration of Sesterterpene MHO7 Derived from a Marine Fungus

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