P116 Neatstik® – a novel point of care test for the measurement of active neutrophil elastase in patients with respiratory disease

Abstract

Introduction and ObjectivesSputum levels of active neutrophil elastase (NE) are frequently elevated in respiratory diseases, such as bronchiectasis, chronic obstructive pulmonary disease (COPD), and cystic fibrosis (CF). Increased NE levels have been demonstrated to inversely correlate with pulmonary function, and risk of exacerbations. Active NE is also recognised as a biomarker of subclinical infection; however, no tools presently exist for its routine monitoring at home or in the clinic. Translation of the ProAxsis NE Activity Based Immunoassay (ABI) to a Point of Care (PoC) device, would facilitate routine monitoring of those patients at highest risk of upcoming exacerbations; enabling pre-emptive medical intervention, and mitigating the patient’s risk of developing serious complications.MethodsActive NE levels in sputum samples (n=10) were determined using the NE ABI (ProAxsis Ltd, Belfast), in accordance with manufacturer’s instructions; followed by assessment using the NEATstik PoC test (threshold 10 µg/ml in sputum). For measurement of NE using NEATstik, sputum was diluted x10, gently rotated for 1 min, an aliquot (70 µl) was then transferred onto the test sample port of the device and allowed to develop for 10 min, after which the signal intensity at the test-line was visually graded (0–10).ResultsNE ABI analysis of sputum revealed that 7 out of 10 samples under investigation contained active NE at levels above the NEATstik test threshold. All 7 samples were found to produce a strong positive test line on the PoC device. Moreover, no test line was visible for the remaining samples with active NE concentrations below 10 µg/ml.ConclusionAvailability of NEATstik, the first highly sensitive and specific PoC test for the rapid detection of active NE in complex clinical samples, should enable the proactive management of multiple chronic respiratory diseases. It has the potential to assist in the identification of patients at highest risk of imminent exacerbations, and thus allow closer monitoring by their clinical team and/or pre-emptive treatment to avoid/minimise the impact of such exacerbations. Additionally, for those presenting with an ongoing exacerbation, the test facilitates patient stratification, with those most likely to respond to antibiotic therapy identified and their response to treatment assessed.

Giant magnetoresistive biosensors for real-time quantitative detection of protease activity

“…The sensor can also be produced in a pre-assembled manner by freeze-drying or lyophilizing the complexes such that it is in a "sample-to-answer" format without the need of additional detection reagents used in traditional ELISA assays. Real-time quantification of protease activity using GMR SV sensor-based detection has significant advantages over current POC protease test kits that detect elevated protease activity in wound fluid 71 or sputum 72 .…”

Giant magnetoresistive biosensors for real-time quantitative detection of protease activity

“…The sensor can also be produced in a pre-assembled manner by freeze-drying or lyophilizing the complexes such that it is in a "sample-to-answer" format without the need of additional detection reagents used in traditional ELISA assays. Real-time quantification of protease activity using GMR SV sensor-based detection has significant advantages over current POC protease test kits that detect elevated protease activity in wound fluid 63 or sputum 64 .…”