2000
DOI: 10.1128/mcb.20.17.6300-6307.2000
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p12DOC-1 Is a Novel Cyclin-Dependent Kinase 2-Associated Protein

Abstract: Regulated cyclin-dependent kinase (CDK) levels and activities are critical for the proper progression of the cell division cycle. p12 DOC-1 is a growth suppressor isolated from normal keratinocytes. We report that p12 DOC-1 associates with CDK2. More specifically, p12 DOC-1 associates with the monomeric nonphosphorylated form of CDK2 (p33CDK2). Ectopic expression of p12 DOC-1 resulted in decreased cellular CDK2 and reduced CDK2-associated kinase activities and was accompanied by a shift in the cell cycle posit… Show more

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Cited by 76 publications
(80 citation statements)
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“…Importantly, after transient transfection of pcDNA3-CDK2-AP1 into CDK2-AP1 deficient cell lines (RKO and SW48), FACS analysis demonstrated a significant decrease in S phase and a significant increase in apoptosis. These results are consistent with previous reports of CDK2-AP1 interaction with DNA polymerase alpha/primase, CDK2, and pRB, genetic elements associated with cell cycle regulation and apoptosis (Tsuji et al, 1998;Shintani et al, 2000). The observations described above, specifically, CDK2-AP1 modulation of apoptosis and S phase in the setting of both microsatellite stable and unstable cell systems, suggests that CDK2-AP1 is a true mediator of cell cycle kinetics and apoptosis independent of microsatellite status.…”
Section: Discussionsupporting
confidence: 82%
“…Importantly, after transient transfection of pcDNA3-CDK2-AP1 into CDK2-AP1 deficient cell lines (RKO and SW48), FACS analysis demonstrated a significant decrease in S phase and a significant increase in apoptosis. These results are consistent with previous reports of CDK2-AP1 interaction with DNA polymerase alpha/primase, CDK2, and pRB, genetic elements associated with cell cycle regulation and apoptosis (Tsuji et al, 1998;Shintani et al, 2000). The observations described above, specifically, CDK2-AP1 modulation of apoptosis and S phase in the setting of both microsatellite stable and unstable cell systems, suggests that CDK2-AP1 is a true mediator of cell cycle kinetics and apoptosis independent of microsatellite status.…”
Section: Discussionsupporting
confidence: 82%
“…CDK2AP1 and Deleted in Oral Cancer -1 (DOC-1), this gene was identified by subtractive hybridization from a hamster oral cancer model [98]. CDK2AP1, a novel cell cycle regulator, has been shown to regulate cell cycle by interacting with CDK2 and preventing phosphorylation of pRB, thus rendering pRB active and able to regulate cell cycle [99]. CDK2AP1 has also been shown to interact with DNA polymerase-α/primase and reduce its activity [100].…”
Section: Genetic Alteration In Preneoplastic Lesionsmentioning
confidence: 99%
“…p12 CDK2-associating protein 1 (p12 CDK2-AP1 ), originally named deleted in oral cancer-1 (DOC-1), therefore acts as a growth suppressor by negatively regulating the activity of cyclin-dependent kinase 2 (CDK2) (1). This gene is highly conserved and located on chromosome 12q24.…”
Section: Introductionmentioning
confidence: 99%