P142 immunological detection of human parathyroid hormone 1–37 (hPTH 1–37), the physiologically circulating fragment of hPTH

Mägerlein, M; Hock, D.; Adermann, Knut; Neidlein, R.; Forssmann, W. G.; Strein, K.

doi:10.1016/0928-0987(94)90315-8

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Immunoenzymetric Assay (Iema)1

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1997

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“…1) were measured by means of IEMA (Fig. 2) to detect the N-terminal parathyroid hormone [18]. This assay detects hPTH-1-84 and fragments of this peptide if the Nterminus is intact (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Isolation and characterization of the bioactive circulating human parathyroid hormone, hPTH‐1–37

Hock¹,

Mägerlein²,

Heine³

et al. 1997

FEBS Letters

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The occurrence of hPTH-1-37 as the native bioactive circulating form of PTH-1 84 has now been obtained using a specific purification procedure for circulating parathyroid hormone, which involves a newly developed immunoenzymetric assay for N-terminally intact hPTH. In combination with two different methods of mass spectrometry, the molecular weight of the isolated immunoreactive peptide was shown to be 4401 Da, which corresponds to hPTH-1-37. Synthetic hPTH-1-37 material was tested in the chick bioassay and produced a clearcut increase in serum calcium concentration. We conclude that hPTH-1-37 is the native bioactive fragment of hPTH-1-84 in circulation.

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“…1) were measured by means of IEMA (Fig. 2) to detect the N-terminal parathyroid hormone [18]. This assay detects hPTH-1-84 and fragments of this peptide if the Nterminus is intact (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The IEMA procedure [18] was carried out in duplicate in microtiter plates (Maxisorb, Nunc, Wiesbaden, Germany). The wells were coated overnight with 0.5 μg/well of a monoclonal antibody.…”

Section: Immunoenzymetric Assay (Iema)mentioning

confidence: 99%