P16/Ki-67 Dual Staining in Positive Human Papillomavirus DNA Testing for Predictive Diagnosis of Abnormal Cervical Lesions in Northeastern Thai Women

Aromseree, Sirinart; Wongjumpa, Weerayut; Ekalaksananan, Tipaya; Temtanakitpaisan, Amornrat; Kleebkaow, Pilaiwan; Srisathaporn, Sawarot; Tongchai, Panwad; Pientong, Chamsai

doi:10.31557/apjcp.2022.23.10.3405

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…The earlier these lesions are detected and appropriately managed, the better the patient outcomes. The p16/Ki-67 dual-staining technique represents a significant step forward in cervical cancer screening, particularly in HPV-positive women [72]. Its superior sensitivity and NPV for detecting high-grade lesions offers the promise of earlier and more accurate identification of women at risk of developing cervical cancer.…”

Section: Study Outcomes On Dual Staining Efficacymentioning

confidence: 99%

Enhancing Cervical Cancer Screening: Review of p16/Ki-67 Dual Staining as a Promising Triage Strategy

Ouh,

Kim,

et al. 2024

Diagnostics

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Cervical cancer, primarily caused by high-risk human papillomavirus (HR-HPV) types 16 and 18, is a major global health concern. Persistent HR-HPV infection can progress from reversible precancerous lesions to invasive cervical cancer, which is driven by the oncogenic activity of human papillomavirus (HPV) genes, particularly E6 and E7. Traditional screening methods, including cytology and HPV testing, have limited sensitivity and specificity. This review explores the application of p16/Ki-67 dual-staining cytology for cervical cancer screening. This advanced immunocytochemical method allows for simultaneously detecting p16 and Ki-67 proteins within cervical epithelial cells, offering a more specific approach for triaging HPV-positive women. Dual staining and traditional methods are compared, demonstrating their high sensitivity and negative predictive value but low specificity. The increased sensitivity of dual staining results in higher detection rates of CIN2+ lesions, which is crucial for preventing cervical cancer progression. However, its low specificity may lead to increased false-positive results and unnecessary biopsies. The implications of integrating dual staining into contemporary screening strategies, particularly considering the evolving landscape of HPV vaccination and changes in HPV genotype prevalence, are also discussed. New guidelines and further research are necessary to elucidate the long-term effects of integrating dual staining into screening protocols.

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Section: Study Outcomes On Dual Staining Efficacymentioning

confidence: 99%

Enhancing Cervical Cancer Screening: Review of p16/Ki-67 Dual Staining as a Promising Triage Strategy

Ouh,

Kim,

et al. 2024

Diagnostics

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Application of p16/Ki-67 double-stained immunohistochemistry in diagnosis of cervical intraepithelial lesions

Artemjeva,

Pakharukova,

Lagereva

2023

Lab. sluzh.

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Combined HPV and CINtec PLUS testing for triaging cervical cancer screening in a Belgian cohort

Louise,

Stefanie,

Kurt

et al. 2023

Ann Cytol Pathol

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Background: Cytological screening with Human Papillomavirus (HPV) triage for equivocal results has been the routine screening procedure for cervical cancer for years worldwide. The dual-marker stain p16/Ki67 (CINtec PLUS) has been shown to offer high sensitivity and specificity in the triage of women at risk of developing HPV-related precancerous lesions. We evaluated the utility of CINtec PLUS in women with normal cytology and a positive HPV test, to see if this test can be used as a prognostic biomarker.  Methods: Women of 18 years or older were assembled between January 2018 and December 2022 at two different study sites. These were cytology negative for intra-epithelial Neoplasia (NILM) and a positive HPV test. The prognostic value of the CINtec PLUS test for NILM samples and the confounding effect of HPV subtype, age, university, and follow-up stage were evaluated.  Results: CINtec PLUS was positive in 63 out of 312 (20%) NILM/ HPV-positive cases. The Positive Predictive Value (PPV) of CINtec PLUS was 92% for high-grade dysplastic lesion (HSIL), 64% for NILM, and 31% for low-grade dysplasia. The negative predictive value was 83% for HSIL, 64% for NILM, and 83% for low-grade dysplasia.  Conclusion: Adding CINtec PLUS with NILM cytology and HPV-positive test can be an important prognostic tool to identify women at risk for a high-grade dysplastic cervical lesion. Importantly, the test can also be used in primary HPV screening programs. However, women with low-grade dysplasia remain at risk for over- and under-treatment.

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P16/Ki-67 Dual Staining in Positive Human Papillomavirus DNA Testing for Predictive Diagnosis of Abnormal Cervical Lesions in Northeastern Thai Women

Cited by 4 publications

References 24 publications

Enhancing Cervical Cancer Screening: Review of p16/Ki-67 Dual Staining as a Promising Triage Strategy

Enhancing Cervical Cancer Screening: Review of p16/Ki-67 Dual Staining as a Promising Triage Strategy

Application of p16/Ki-67 double-stained immunohistochemistry in diagnosis of cervical intraepithelial lesions

Combined HPV and CINtec PLUS testing for triaging cervical cancer screening in a Belgian cohort

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