p166, a link between the trypanosome mitochondrial DNA and flagellum, mediates genome segregation

Zhao, Zhixing; Lindsay, Megan E.; Chowdhury, Arnab Roy; Robinson, Derrick R.; Englund, Paul T.

doi:10.1038/sj.emboj.7601956

Cited by 80 publications

(132 citation statements)

References 36 publications

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“…This suggests that the entire kDNA network is overreplicated in the induced RNAi cell line, indicating that TAC40 is required for segregation of kDNA networks but not for their replication. The kDNA segregation defect and the associated overreplication of the remaining kDNA are reminiscent of what has been observed in cells ablated for p166 and p197, two previously characterized TAC components (8,10).…”

Section: Resultsmentioning

confidence: 69%

“…It consists of a disk-shaped single-unit kDNA network that localizes to a distinct region within the mitochondrial matrix (6). The kDNA is physically connected with the cytosolic basal body, the organizing center of the eukaryotic flagellum, via a highorder transmembrane structure termed tripartite attachment complex (TAC) (7) of which only few components have been identified (8)(9)(10). Replication of the kDNA network occurs at a defined stage of the cell cycle shortly before the onset of the nuclear S phase.…”

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confidence: 99%

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Trypanosomal TAC40 constitutes a novel subclass of mitochondrial β-barrel proteins specialized in mitochondrial genome inheritance

Schnarwiler

Niemann

Doiron

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Mitochondria cannot form de novo but require mechanisms allowing their inheritance to daughter cells. In contrast to most other eukaryotes Trypanosoma brucei has a single mitochondrion whose single-unit genome is physically connected to the flagellum. Here we identify a β-barrel mitochondrial outer membrane protein, termed tripartite attachment complex 40 (TAC40), that localizes to this connection. TAC40 is essential for mitochondrial DNA inheritance and belongs to the mitochondrial porin protein family. However, it is not specifically related to any of the three subclasses of mitochondrial porins represented by the metabolite transporter voltage-dependent anion channel (VDAC), the protein translocator of the outer membrane 40 (TOM40), or the fungi-specific MDM10, a component of the endoplasmic reticulum-mitochondria encounter structure (ERMES). MDM10 and TAC40 mediate cellular architecture and participate in transmembrane complexes that are essential for mitochondrial DNA inheritance. In yeast MDM10, in the context of the ERMES, is postulated to connect the mitochondrial genomes to actin filaments, whereas in trypanosomes TAC40 mediates the linkage of the mitochondrial DNA to the basal body of the flagellum. However, TAC40 does not colocalize with trypanosomal orthologs of ERMES components and, unlike MDM10, it regulates neither mitochondrial morphology nor the assembly of the protein translocase. TAC40 therefore defines a novel subclass of mitochondrial porins that is distinct from VDAC, TOM40, and MDM10. However, whereas the architecture of the TAC40-containing complex in trypanosomes and the MDM10-containing ERMES in yeast is very different, both are organized around a β-barrel protein of the mitochondrial porin family that mediates a DNA-cytoskeleton linkage that is essential for mitochondrial DNA inheritance.organelle | parasitic protozoa

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Section: Resultsmentioning

confidence: 69%

mentioning

confidence: 99%

Trypanosomal TAC40 constitutes a novel subclass of mitochondrial β-barrel proteins specialized in mitochondrial genome inheritance

Schnarwiler

Niemann

Doiron

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Since the basal bodies are physically linked to the kinetoplast, the motor could lie in the kinetoplast; however, we and others have previously shown this to be unlikely, because basal bodies can segregate in the absence of kinetoplast segregation (Ploubidou et al, 1999;Robinson and Gull, 1991;Zhao et al, 2008). However, there might be implications for kinetoplast organisation from this morphogenetic rotation of the new basal body.…”

Section: Basal Body Migration and Cell Morphogenesismentioning

confidence: 98%

Basal body movements orchestrate membrane organelle division and cell morphogenesis inTrypanosoma brucei

Lacomble

Vaughan

Gadelha

et al. 2010

Journal of Cell Science

144

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SummaryThe defined shape and single-copy organelles of Trypanosoma brucei mean that it provides an excellent model in which to study how duplication and segregation of organelles is interfaced with morphogenesis of overall cell shape and form. The centriole or basal body of eukaryotic cells is often seen to be at the centre of such processes. We have used a combination of electron microscopy and electron tomography techniques to provide a detailed three-dimensional view of duplication of the basal body in trypanosomes. We show that the basal body duplication and maturation cycle exerts an influence on the intimately associated flagellar pocket membrane system that is the portal for secretion and uptake from this cell. At the start of the cell cycle, a probasal body is positioned anterior to the basal body of the existing flagellum. At the G1-S transition, the probasal body matures, elongates and invades the pre-existing flagellar pocket to form the new flagellar axoneme. The new basal body undergoes a spectacular anti-clockwise rotation around the old flagellum, while its short new axoneme is associated with the pre-existing flagellar pocket. This rotation and subsequent posterior movements results in division of the flagellar pocket and ultimately sets parameters for subsequent daughter cell morphogenesis.

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“…Due to this connection, the position of the kinetoplast defines the cellular region in which the basal body is located and, consequently, the origin of the flagellum. Recently, a protein designated p166 was identified and shown to be located in between the K-DNA disk and the flagellar body (Zhao et al 2008).…”

Section: The Kinetoplast-mitochondrion Complexmentioning

confidence: 99%

Structural organization of Trypanosoma cruzi

Souza

2009

Mem. Inst. Oswaldo Cruz

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p166, a link between the trypanosome mitochondrial DNA and flagellum, mediates genome segregation

Cited by 80 publications

References 36 publications

Trypanosomal TAC40 constitutes a novel subclass of mitochondrial β-barrel proteins specialized in mitochondrial genome inheritance

Trypanosomal TAC40 constitutes a novel subclass of mitochondrial β-barrel proteins specialized in mitochondrial genome inheritance

Basal body movements orchestrate membrane organelle division and cell morphogenesis inTrypanosoma brucei

Structural organization of Trypanosoma cruzi

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