P1B-ATPases – an ancient family of transition metal pumps with diverse functions in plants

Williams, Lorraine E.; Mills, Rebecca F.

doi:10.1016/j.tplants.2005.08.008

Cited by 365 publications

(349 citation statements)

References 60 publications

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“…A discontiguous MegaBLAST search did not retrieve sequences homologous to the divalent cation-transporting P 1B -type ATPases in the moss Physcomitrella patens in the NCBI trace archives (January 2007). Similarly, unicellular algae only possess the Cu(I)-transporting P IB -type ATPases that are involved in copper delivery to the chloroplast or the ER, but lack the divalent cation-transporting HMAs [17,21].…”

Section: Long-distance Transportmentioning

confidence: 99%

“…These contain several cysteine dipeptides, and the C-termini of AtHMA2 and AtHMA4 additionally contain long stretches of histidine residues. These domains constitute putative metal-binding sites and might serve as heavy metal sensors or domains for interactions with regulatory proteins [17,77]. Interestingly, although the core domains of the HMA4 proteins are highly similar, the HMA4 cytosolic C-terminal domains of both hyperaccumulators A. halleri and T. caerulescens exhibit strong sequence differences compared to their A. thaliana homolog [44,56,57].…”

Section: Putative Regulatory Domains In Transition Metal Transportersmentioning

confidence: 99%

“…Finally, putative transporters of metal ion ligands, the AtFRD3 (Ferric Reductase Defective 3) of the large multidrug and toxin efflux (MATE) family and AtZIF1 (Zinc Induced Facilitator 1) of the major facilitator superfamily (MFS), have key roles in iron and Zn homeostasis, respectively [11][12][13]. These plant metal transporter families and their biological roles have been reviewed by other authors recently [2,[14][15][16][17][18][19][20][21] (review Iron Transport, this issue).…”

Section: Introductionmentioning

confidence: 99%

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Transition metal transport

2007

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Transition metal transporters are of central importance in the plant metal homeostasis network which maintains internal metal concentrations within physiological limits. An overview is given of the functions of known transition metal transporters in the context of the unique chemical properties of their substrates. The modifications of the metal homeostasis network associated with the adaptation to an extreme metalliferous environment are illustrated in two Brassicaceae metal hyperaccumulator model plants based on cross-species transcriptomics studies. In a comparison between higher plants and unicellular algae, hypotheses are generated for evolutionary changes in metal transporter complements associated with the transition to multicellularity.

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Section: Long-distance Transportmentioning

confidence: 99%

Section: Putative Regulatory Domains In Transition Metal Transportersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transition metal transport

2007

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“…A number of different transporters, such as Cation Diffusion Facilitator, Natural resistance-associated macrophage protein, ATPBinding Cassette, Zinc-and Iron-regulated-like Protein, and P-type ATPase, have been reported to be involved in the uptake, translocation, distribution, and homeostasis of nutrients (Hall and Williams, 2003;Krämer et al, 2007;Palmer and Guerinot, 2009). Among them, heavy metal-transporting P-type ATPase (HMA), the P 1B subfamily of the P-type ATPase superfamily, has been implicated in heavy metal transport (Williams and Mills, 2005;Grotz and Guerinot, 2006;Argüello et al, 2007;Burkhead et al, 2009). There are eight and nine members of P 1B -ATPase in Arabidopsis thaliana and rice (Oryza sativa), respectively (Williams and Mills, 2005).…”

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confidence: 99%

A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

et al. 2013

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ORCID ID: 0000-0003-3411-827X (J.F.M.).Heavy metal-transporting P-type ATPase (HMA) has been implicated in the transport of heavy metals in plants. Here, we report the function and role of an uncharacterized member of HMA, OsHMA5 in rice (Oryza sativa). Knockout of OsHMA5 resulted in a decreased copper (Cu) concentration in the shoots but an increased Cu concentration in the roots at the vegetative stage. At the reproductive stage, the concentration of Cu in the brown rice was significantly lower in the mutants than in the wild-type rice; however, there was no difference in the concentrations of iron, manganese, and zinc between two independent mutants and the wild type. The Cu concentration of xylem sap was lower in the mutants than in the wild-type rice. OsHMA5 was mainly expressed in the roots at the vegetative stage but also in nodes, peduncle, rachis, and husk at the reproductive stage. The expression was up-regulated by excess Cu but not by the deficiency of Cu and other metals, including zinc, iron, and manganese, at the vegetative stage. Analysis of the transgenic rice carrying the OsHMA5 promoter fused with green fluorescent protein revealed that it was localized at the root pericycle cells and xylem region of diffuse vascular bundles in node I, vascular tissues of peduncle, rachis, and husk. Furthermore, immunostaining with an antibody against OsHMA5 revealed that it was localized to the plasma membrane. Expression of OsHMA5 in a Cu transport-defective mutant yeast (Saccharomyces cerevisiae) strain restored the growth. Taken together, OsHMA5 is involved in loading Cu to the xylem of the roots and other organs.

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“…Some of the metal transporter genes are transcriptionally activated upon treatment with its stressor. AtHMA4, a Zn-transporting ATPase, is up-regulated when Zn levels increased (Williams and Mills, 2005), and IRT1, Fe transporter, is up-regulated under low Fe conditions (Vert et al, 2001). Whereas, MTP1/ZAT transcript levels were not altered by metals (van der Zaal et al, 1999), and transcript levels of AtMTP11, cation diffusion facilitator (CDF) family protein which transports manganese, were not increased in COL 0 wild-type Arabidopsis plant under toxic manganese stress (Sanders et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast

Bozdag

Kaya

Koç

et al. 2014

Gene

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Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

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P1B-ATPases – an ancient family of transition metal pumps with diverse functions in plants

Cited by 365 publications

References 60 publications

Transition metal transport

Transition metal transport

A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast

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