P2-063: Clinical and prognostic significance of serum carcinoembryonic antigen level in patients with IIIB and IV stage non-small cell lung cancer: A prospective study
Abstract:Methods: Blood, plasma and serum samples were spiked with known quantities of human DNA. To optimize the yield of DNA, we investigated the effects of changes in the blood collection and processing, storage and DNA extraction protocols. We compared two DNA purification methods, the QIAamp blood DNA kit (QIAGEN) (with and without proteinase K pre-digestion) and phenol/chloroform DNA extraction after heat denaturation (5 minutes at 95˚C). Extracted DNA was quantified using the PicoGreen assay and its quality was … Show more
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