P2.1.16 Optimization of physicochemical parameters of a multilayered polyelectrolyte film deposition with Love wave and AFM for bacteria based detection of heavy metals

Analytical Protocols for Separation and Electron Microscopy of Nanoparticles Interacting with Bacterial Cells

“…The current approaches tend to primarily focus on biological or physical aspects of such samples. A commonly used biological assay, for example, tests the antimicrobial effect of NPs by evaluating the bacterial viability after exposure to NPs. , Physical aspects of NP–bacteria interactions tend to be analyzed by high-resolution imaging techniques, such as electron, ,,,, atomic force, or Raman microscopies, which can provide direct and quantitative information about NPs and cells (such as proximity, penetration, aggregation) but typically not directly in solution environments where NPs and bacteria interact. Artifacts introduced during preparation for imaging, therefore, can obscure evidence of NP–bacteria interactions.…”