P2‐172: Hydrocortisone Cytorestores Oxidative Stress‐induced Neuroblastoma

Rossato, Rafaella Carvalho; Pinto, Jéssica Cristina; Moraes, Carlos Dailton Guedes de Oliveira; Salles, Geisa Nogueira; Pacheco‐Soares, Cristina

doi:10.1016/j.jalz.2019.06.2579

Cited by 3 publications

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“…SH-SY5Y cells were subjected to 200 µM hydrocortisone (Teuto) as stressor agent, dissolved in DMEM/F12 (Gibco -Dulbecco's Modified Eagle Medium -Nutrient Mixture), supplemented with 10 % fetal bovine serum (Life Technologies) and 1 % antibiotic and antimycotic (penicillin and streptomycin -Thermo Fisher Scientific, Invitrogen) (Rossato et al, 2019).…”

Section: Oxidative Stress Inductionmentioning

confidence: 99%

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Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress

Rossato

Granato

Moraes

et al. 2021

RSD

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Alzheimer's disease (AD) is the most common, progressive and irreversible neurodegenerative disorder, characterized by memory loss, cognitive impairment and behavioral abnormalities. Although there is no cure, several study strategies seek to elucidate mechanisms of the disease. Recent studies address the benefits of taurine. Thus, the present study aims to analyze neuroprotective effects of taurine in human neuroblastoma (SH-SY5Y), using an in vitro experimental model of oxidative stress induced by hydrocortisone. This work showed for the first time that taurine can promote neuroprotection in SH-SY5Y under oxidative stress caused by hydrocortisone. Cell viability was evaluated using crystal violet and the evaluation of cell morphology was performed by scanning electron microscopy (SEM). The viability of SH-SY5Y pre-treated with taurine and stressed with hydrocortisone was preserved, compared to the stressed only group, which was also morphologically observed. Therefore, taurine can represent a considerable therapeutic candidate in the prevention of neurodegenerative diseases, such as AD.

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Section: Oxidative Stress Inductionmentioning

confidence: 99%

“…Hydrocortisone, a drug analogous to the hormone cortisol, can generate ROS, especially in high concentrations, damaging important biomolecules (Rossato et al, 2019). Neural cell's normal functioning can be compromised when these damages are not repaired.…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress

Rossato

Granato

Moraes

et al. 2021

RSD

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“…Hydrocortisone, a drug analogous to the hormone cortisol, can generate ROS, especially in high concentrations. Thus, it is worth mentioning the damage caused to biomolecules (ROSSATO et al, 2019). Neural cell's normal functioning can be compromised when these damages are not repaired.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Taurine’s Neuroprotective Effects on Sh-Sy5y Cells Under Oxidative Stress

Rossato

Granato

Pinto

et al. 2021

Preprint

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Alzheimer’s disease (AD) is a type of dementia that affects millions of people. Although there is no cure, several study strategies seek to elucidate the mechanisms of the disease. Recent studies address the benefits of taurine. Thus, the present study aims to analyze the neuroprotective effect of taurine on human neuroblastoma, using an in vitro experimental model of oxidative stress induced by hydrocortisone in the SH-SY5Y cell line as a characteristic model of AD. The violet crystal assay was used for cell viability and the evaluation of cell morphology was performed by scanning electron microscopy (SEM). After pretreatment with taurine, the SH-SY5Y cell showed an improvement in cell viability in the face of oxidative stress and improved cell morphology. Thus, the treatment presented a neuroprotective effect.GRAPHICAL ABSTRACT

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Efeitos Neuroprotetores Da Hidrocortisona Em Linhagem De Neuroblastoma De Murino (Neuro-2a) Sob Estresse Oxidativo

et al. 2020

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A Doença de Alzheimer é um tipo de demência que acomete milhões de pessoas, sendo responsável por 60% de todos os casos de doenças neurodegenerativas. Embora não tenha cura, diversas estratégias de estudo vêm sendo desenvolvidas a fim de elucidar os mecanismos da doença. Estudos recentes abordam os benefícios do cortisol em aspectos imunológicos, musculares, renais, respostas inflamatórias e até mesmo em patologias neurodegenerativas. A hidrocortisona é um medicamento sintético utilizado para simular o cortisol. Deste modo, o estudo explora os efeitos da hidrocortisona na senescência neural, objetivando analisar seu efeito neuroprotetor. Assim, desenvolveu-se um modelo experimental in vitro de estresse oxidativo, induzido por peróxido de hidrogênio na linhagem celular Neuro-2a (Neuroblastoma de Murino) a fim de simular aspectos característicos da Doença de Alzheimer. Foram então realizados experimentos de viabilidade e morfologia celular. A hidrocortisona em baixa concentração promoveu aumento na viabilidade celular, enquanto que o peróxido de hidrogênio diminuiu a viabilidade celular, ocorrendo o efeito de estresse oxidativo. No aspecto morfológico, a hidrocortisona preservou os prolongamentos das células, já o peróxido de hidrogênio fez com que os prolongamentos retraíssem, assim perdendo as sinapses. Com os resultados obtidos, pode-se concluir que a hidrocortisona preservou a célula neural do efeito do estresse oxidativo.

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P2‐172: Hydrocortisone Cytorestores Oxidative Stress‐induced Neuroblastoma

Cited by 3 publications

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Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress

Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress

Evaluation of Taurine’s Neuroprotective Effects on Sh-Sy5y Cells Under Oxidative Stress

Efeitos Neuroprotetores Da Hidrocortisona Em Linhagem De Neuroblastoma De Murino (Neuro-2a) Sob Estresse Oxidativo

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