2003
DOI: 10.4161/cc.2.6.502
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p21CDKN1A Does Not Interfere with Loading of PCNA at DNA Replication Sites, but Inhibits Subsequent Binding of DNA Polymerase D at the G1/S Phase Transition

Abstract: The ability of the cyclin-dependent kinase (CDK) inhibitor p21 CDKN1A to interact with PCNA recruited to DNA replication sites was investigated to elucidate the relevance of this interaction in cell cycle arrest. To this end, expression of p21 protein fused to green fluorescent protein (GFP) was induced in HeLa cells. G 1 phase cell cycle arrest induced by p21GFP occurred also at the G 1 /S transition, as shown by cyclin A immunostaining of GFP-positive cells. Confocal microscopy analysis and co-immunoprecipit… Show more

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Cited by 53 publications
(52 citation statements)
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“…Downregulation of CDC2, CCNB1, AURKA/AURKB, and TPX2 has been reported to induce cell cycle arrest in the G2 phase, [39][40][41] and downregulation of PCNA led to cell cycle arrest in the G1 phase. 20,38 Among these genes, CDC2, CCNB1, AURKA/AURKB, and TPX2 were also downregulated by the supernatant, but PCNA was not changed by the supernatant (Table 1). We observed a marked increase in the number of cells in the G2/M phase upon exposure to CuO-NPs and the supernatant compared with control cells (Figure 8).…”
Section: Contribution Of Cu Ionsmentioning
confidence: 96%
See 1 more Smart Citation
“…Downregulation of CDC2, CCNB1, AURKA/AURKB, and TPX2 has been reported to induce cell cycle arrest in the G2 phase, [39][40][41] and downregulation of PCNA led to cell cycle arrest in the G1 phase. 20,38 Among these genes, CDC2, CCNB1, AURKA/AURKB, and TPX2 were also downregulated by the supernatant, but PCNA was not changed by the supernatant (Table 1). We observed a marked increase in the number of cells in the G2/M phase upon exposure to CuO-NPs and the supernatant compared with control cells (Figure 8).…”
Section: Contribution Of Cu Ionsmentioning
confidence: 96%
“…[20][21][22] In contrast, CDKN1A inhibits PCNA and blocks the transition from the G1 to the S phase of the cell cycle. 20,38 The CDC2-CCNB1 complex is required for the transition from the G2 to the M phase. 39,40 Although CuO-NPs did not affect the expression of CDKN1A, they downregulated the expression of PCNA, CDC2, and CCNB1 ( Figure 4b, Table 1), which suggests that CuO-NPs induce cell cycle arrest in the G1 and G2 phases.…”
Section: Molecularmentioning
confidence: 99%
“…Cip through the same region, p21 Cip thus impairs PCNA association to Pol␦/ (22,24). We examined PCNA-p21 Cip complex formation in cells with altered p110␤ activity.…”
Section: The P110␤ Is Located In the Nucleus And Controls Pcna Bindinmentioning
confidence: 99%
“…It was suggested that the interaction between pol δ and p21 might be bridged by PCNA due to the trimetric structure of PCNA. 35 Their observation could also be explained if there is a direct interaction between p21 and the p50 subunits of pol δ.…”
Section: Discussionmentioning
confidence: 99%
“…The association between p21 and the pol δ complex has been observed by other investigators. 35 GFP-p21 could coimmunoprecipitate p125-pol δ from the detergent-soluble fraction of cell lysates. It was suggested that the interaction between pol δ and p21 might be bridged by PCNA due to the trimetric structure of PCNA.…”
Section: Discussionmentioning
confidence: 99%