P24 immunohistochemistry on lymphoid tissue: the histopathologist’s role in HIV diagnosis

Mahadeva, Ula; Walt, Jon van der; Moonim, Mufaddal; Lucas, Sebastian

doi:10.1111/j.1365-2559.2010.03491.x

Cited by 10 publications

(6 citation statements)

References 44 publications

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“…Hormis les colorations histochimiques, ces méthodes utilisent les techniques d'immunohistochimie et d'hybridation in situ. Ainsi, un certain nombre d'anticorps dirigés spécifiquement contre les antigènes d'agents pathogènes sont aisément utilisables sur des coupes déparaffinées [46,48,[69][70][71][72]. Toutefois, la liste des anticorps commercialisés est actuellement réduite, ceci se limitant aux agents pathogènes les plus fréquents.…”

Section: Améliorer L'apport Des Méthodes Complémentaires En Pathologiunclassified

Quelle place et quel avenir pour l’anatomo-cyto-pathologie des maladies infectieuses et tropicales en France ?

Hofman

2014

Annales de Pathologie

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The management of tissues and cellular samples by the pathologists in the infectious and tropical diseases pathology field in 2014 needs a strong knowledge of both morphological and molecular domains which includes the good control: (i) of the taxonomy of infectious and tropical diseases pathology leading to the pathogens identification and (ii) of the ancillary methods which can be used in fixed samples in order to detect or better identify these pathogens. There is a recent paradox in France concerning the frequency of infectious diseases to be diagnosed in pathology laboratories and the progressive loss of pathologist's expertise in this domain. Different reasons could explain this statement including the omnipresence of the tumour lesions to be managed in a pathology laboratory as well as the recent constraints associated with the different biomarkers that are mandatory to be detected by immunohistochemistry and/or by molecular biology. Even if the microbiologists play a pivotal role for identifying the different pathogens as well as for the assessment of their sensitivity to the anti-microbial drugs, a large number of infectious diseases can be diagnosed only on fixed tissue and/or cells by the pathologists. The purpose of this review is to describe the current and future issues of infectious and tropical diseases diagnoses in pathology laboratories, in particular in France.

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Section: Améliorer L'apport Des Méthodes Complémentaires En Pathologiunclassified

Quelle place et quel avenir pour l’anatomo-cyto-pathologie des maladies infectieuses et tropicales en France ?

Hofman

2014

Annales de Pathologie

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“…Recently, Epstein–Barr virus‐encoded ribonucleic acid (RNA) was demonstrated using in situ hybridization (EBER‐ISH) in some of the cases, but immunohistochemistry (IHC) for human herpes virus 8‐latency‐associated nuclear antigen [HHV‐8‐latency‐associated nuclear antigen (LANA)] was negative 2 . P24 has also been described in HIV infection in lymphoid and non‐lymphoid tissue 1 …”

Section: Introductionmentioning

confidence: 99%

“…2 P24 has also been described in HIV infection in lymphoid and non-lymphoid tissue. 1 The histopathology of HIV lymphadenitis has been considered to be non-specific. 3 However, other authors argue that the changes are sufficiently characteristic to permit a tentative diagnosis of HIV lymphadenopathy in the presence of relevant clinical and immunological manifestations, which should be subsequently confirmed by serological testing.…”

Section: Introductionmentioning

confidence: 99%

The incidence of human herpes virus‐8 expression in lymph node biopsies from human immunodeficiency virus‐positive patients

Beukes

Thiart

2012

Histopathology

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Beukes C A & Thiart J  (2012) Histopathology 61, 942–944 The incidence of human herpes virus‐8 expression in lymph node biopsies from human immunodeficiency virus‐positive patients Aims: Human immunodeficiency virus (HIV)‐related lymphadenopathy is characterized by a wide spectrum of histological changes. Three patterns have been described which correspond to clinical stages of HIV/acquired immune deficiency syndrome (AIDS). Castleman disease is a heterogeneous group of disorders. A recently described variant, multicentric Castleman disease (MCD), of which some cases are associated with human herpes virus‐8 (HHV‐8), has been reported in both HIV‐seropositive and ‐negative patients. Considerable morphological overlap occurs between one of the patterns of HIV lymphadenopathy and this variant. Methods and results: This retrospective histopathological study on 95 cases of HIV‐reactive lymphadenopathy assessed the incidence of the different patterns and HHV‐8 on immunohistochemistry (IHC). Of the 95 cases, 78 (82.1%) were HHV‐8‐negative, of which 46 (59.0%) were classified as pattern A, 20 (25.6%) as pattern B and 12 (15.4%) as pattern C. Nine (31.0%) of 29 cases with pattern B and 8 (40.0%) of 20 cases with pattern C were HHV‐8 positive. In total 15 cases of MCD were diagnosed in this series. Conclusion: This study draws attention to the overlap between HIV lymphadenopathy and MCD. We recommend that cases of HHV‐8‐associated MCD should be investigated for HIV infection.

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“…With immunohistochemistry, it is possible to identify HIV within tissue cells, using antibodies to p24 gag core protein. We have encouraged pathologists to use this technique on lymphoid tissue biopsies in order to identify persons previously undiagnosed with HIV 1,2 . Whilst there are some publications on the application of HIVp24 immunohistochemistry at autopsy, particularly to investigate brain pathology, there are no reported examples of its application in autopsy cytopathology.…”

mentioning

confidence: 99%

“…We have encouraged pathologists to use this technique on lymphoid tissue biopsies in order to identify persons previously undiagnosed with HIV. 1,2 Whilst there are some publications on the application of HIVp24 immunohistochemistry at autopsy, particularly to investigate brain pathology, there are no reported examples of its application in autopsy cytopathology. I present an example of florid splenic HIV infection using cytopathology, with histopathology confirmation.…”

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confidence: 99%

Splenic HIV infection diagnosed with p24 on autopsy cytopathology confirmed on histopathology

Lucas

2011

Cytopathology

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P24 immunohistochemistry on lymphoid tissue: the histopathologist’s role in HIV diagnosis

Cited by 10 publications

References 44 publications

Quelle place et quel avenir pour l’anatomo-cyto-pathologie des maladies infectieuses et tropicales en France ?

Quelle place et quel avenir pour l’anatomo-cyto-pathologie des maladies infectieuses et tropicales en France ?

The incidence of human herpes virus‐8 expression in lymph node biopsies from human immunodeficiency virus‐positive patients

Splenic HIV infection diagnosed with p24 on autopsy cytopathology confirmed on histopathology

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