P27 
Comparison of ninhydrine methods of detecting amine compounds with other methods of detection in halitosis

Abstract

Objectives Availability of required substrate – amino acids is a factor determining the appearance of halitosis. The aim of the study was to determine whether a chemical test for low molecular weight amines in the saliva may be a new method of diagnosing halitosis. Methods Eighty‐four patients suffering from halitosis and 40 healthy volunteers aged 20–62 (avg. 39.7) participated in the study. Halitosis was diagnosed if the average level of VSC measured by a halimeter was >125 ppb and the organoleptic measurement using a 5‐point scale was >2. In all subjects low molecular weight amines were evaluated by the ninhydrine method. Patients with halitosis were randomized into a treatment groups. Zinc tablets, tablets and mouthwash containing chlorhexidine or lyophilized lactic acid‐forming bacteria were used. Test subjects were examined at day 0, 7, 14, 21 and after 3 months and controls on day 0, 21 and after 3 months. Statistical analysis was performed using Wilcoxon's test. Results Analysis showed, that the level of amines was highest in subjects with halitosis (0.39, s.d. 0.06) and correlated significantly with results of VSC measurement and organoleptic scores (P < 0.001). Reduction of amine levels after treatment was statistically significant (0.36, s.d. 0.06, P < 0.001). The amine levels in healthy controls was lower (0.29, s.d. 0.07) and remained at a similar, stable level. Conclusions The ninhydrin method of detecting salivary amines may be one of the methods of diagnosing halitosis, because salivary amine levels significantly correlated with VSC levels measured by halimeter and organoleptic scores. This method may also be used to evaluate treatment efficacy.

Oral Probiotik: Pendekatan Baru Terapi Halitosis

“…Secara umum, diagnosis halitosis dapat dilakukan dengan identifikasi kadar bahan volatil yang dihasilkan dan identifikasi mikroba penyebab halitosis. Penggunaan halimeter yang berfungsi mengukur kadar sulfida volatil 14 , tes BANA (N-benzoyl-DL-arginine-2naphthylamide) yang mengukur kadar sulfida sulkus gingiva 15 , kromatografi gas 16 , pengukuran dengan organoleptik 16 , electronic nose 16 , pemeriksaan kadar salivary βgalactosidase 14 , metoda ninhydrine (kadar amin saliva) 17 , inkubasi saliva 18 , cysteine challenge testing 19 merupakan beberapa cara identifikasi kadar bahan volatil penyebab halitosis.…”

“…Metode diagnosis halitosis dikelompokkan menjadi identifikasi bahan volatil dan identifikasi mikroba. [14][15][16][17][18][19][20] Identifikasi bahan volatil…”